Restriction map for
pFN21AE3977
(Linear) (Six-base) MAPSORT of: KIEE3977.seq Check: 8781 from: 1 to: 774
KIEE3977.orf
With 182 enzymes: SgfI *
March 22, 2012 17:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 475 774
Size: 475 299
AleI CACnn'nnGTG
Cuts at: 0 50 550 774
Size: 50 500 224
Fragments arranged by size:
500 224 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 68 774
Size: 68 706
ApaLI G'TGCA_C
Cuts at: 0 247 774
Size: 247 527
ApoI r'AATT_y
Cuts at: 0 526 774
Size: 526 248
AvaI C'yCGr_G
Cuts at: 0 556 774
Size: 556 218
BanI G'GyrC_C
Cuts at: 0 237 716 774
Size: 237 479 58
Fragments arranged by size:
479 237 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 461 774
Size: 461 313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 300 334 774
Size: 300 34 440
Fragments arranged by size:
440 300 34
BglI GCCn_nnn'nGGC
Cuts at: 0 223 627 774
Size: 223 404 147
Fragments arranged by size:
404 223 147
BglII A'GATC_T
Cuts at: 0 170 774
Size: 170 604
BlpI GC'TnA_GC
Cuts at: 0 704 774
Size: 704 70
Bme1580I G_kGCm'C
Cuts at: 0 20 251 557 774
Size: 20 231 306 217
Fragments arranged by size:
306 231 217 20
BmgBI CAC'GTC
Cuts at: 0 552 774
Size: 552 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 774
Size: 253 521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 774
Size: 139 635
BsaBI GATnn'nnATC
Cuts at: 0 514 774
Size: 514 260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 774
Size: 430 30 314
Fragments arranged by size:
430 314 30
BseYI C'CCAG_C
Cuts at: 0 664 774
Size: 664 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 774
Size: 344 430
BsiHKAI G_wGCw'C
Cuts at: 0 251 768 774
Size: 251 517 6
Fragments arranged by size:
517 251 6
BsmI GAATG_Cn'
Cuts at: 0 80 774
Size: 80 694
Bsp1286I G_dGCh'C
Cuts at: 0 20 251 557 768 774
Size: 20 231 306 211 6
Fragments arranged by size:
306 231 211 20 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 475 774
Size: 475 299
BsrBI CCG'CTC
Cuts at: 0 123 561 774
Size: 123 438 213
Fragments arranged by size:
438 213 123
BsrFI r'CCGG_y
Cuts at: 0 98 262 774
Size: 98 164 512
Fragments arranged by size:
512 164 98
BssSI C'ACGA_G
Cuts at: 0 46 774
Size: 46 728
BstYI r'GATC_y
Cuts at: 0 170 774
Size: 170 604
BtgI C'CryG_G
Cuts at: 0 252 278 774
Size: 252 26 496
Fragments arranged by size:
496 252 26
BtsI GCAGTG_nn'
Cuts at: 0 155 774
Size: 155 619
EaeI y'GGCC_r
Cuts at: 0 2 124 215 289 671 774
Size: 2 122 91 74 382 103
Fragments arranged by size:
382 122 103 91 74 2
EarI CTCTTCn'nnn_
Cuts at: 0 181 424 430 607 774
Size: 181 243 6 177 167
Fragments arranged by size:
243 181 177 167 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 774
Size: 253 521
EcoO109I rG'GnC_Cy
Cuts at: 0 492 564 774
Size: 492 72 210
Fragments arranged by size:
492 210 72
FspI TGC'GCA
Cuts at: 0 88 774
Size: 88 686
HaeII r_GCGC'y
Cuts at: 0 211 774
Size: 211 563
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 774
Size: 430 32 312
Fragments arranged by size:
430 312 32
MscI TGG'CCA
Cuts at: 0 4 217 291 774
Size: 4 213 74 483
Fragments arranged by size:
483 213 74 4
MslI CAynn'nnrTG
Cuts at: 0 50 550 774
Size: 50 500 224
Fragments arranged by size:
500 224 50
MspA1I CmG'CkG
Cuts at: 0 471 711 774
Size: 471 240 63
Fragments arranged by size:
471 240 63
NaeI GCC'GGC
Cuts at: 0 100 774
Size: 100 674
NdeI CA'TA_TG
Cuts at: 0 380 774
Size: 380 394
NgoMIV G'CCGG_C
Cuts at: 0 98 774
Size: 98 676
NspI r_CATG'y
Cuts at: 0 543 774
Size: 543 231
PfoI T'CCnGG_A
Cuts at: 0 181 774
Size: 181 593
PpuMI rG'GwC_Cy
Cuts at: 0 564 774
Size: 564 210
PstI C_TGCA'G
Cuts at: 0 305 774
Size: 305 469
PvuII CAG'CTG
Cuts at: 0 471 711 774
Size: 471 240 63
Fragments arranged by size:
471 240 63
SexAI A'CCwGG_T
Cuts at: 0 725 774
Size: 725 49
SfcI C'TryA_G
Cuts at: 0 301 774
Size: 301 473
SgrAI Cr'CCGG_yG
Cuts at: 0 98 774
Size: 98 676
SmlI C'TyrA_G
Cuts at: 0 118 774
Size: 118 656
StuI AGG'CCT
Cuts at: 0 586 774
Size: 586 188
TatI w'GTAC_w
Cuts at: 0 536 774
Size: 536 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 177 209 774
Size: 177 32 565
Fragments arranged by size:
565 177 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 691 733 774
Size: 691 42 41
Fragments arranged by size:
691 42 41
Enzymes that do cut:
AarI AleI AlfI ApaLI ApoI AvaI BanI BbsI
BcgI BglI BglII BlpI Bme1580I BmgBI BpmI BpuEI
BsaBI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BssSI BstYI BtgI BtsI EaeI EarI
Eco57MI EcoO109I FspI HaeII Hin4I MscI MslI MspA1I
NaeI NdeI NgoMIV NspI PfoI PpuMI PstI PvuII
SexAI SfcI SgrAI SmlI StuI TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbvCI BciVI BclI
BfrBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI
BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NcoI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI TaqII Tth111I XbaI
XhoI XmaI XmnI ZraI