(Linear) (Six-base) MAPSORT of: KIEE3970.seq  Check: 8134  from: 1  to: 459



KIEE3970.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     155     459 
   Size:        155     304
 
ApoI r'AATT_y

Cuts at:      0     244     330     459 
   Size:        244      86     129

  Fragments arranged by size: 

                 244     129      86
 
BanII G_rGCy'C

Cuts at:      0      80     221     459 
   Size:         80     141     238

  Fragments arranged by size: 

                 238     141      80
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     119     459 
   Size:        119     340
 
BlpI GC'TnA_GC

Cuts at:      0     215     319     459 
   Size:        215     104     140

  Fragments arranged by size: 

                 215     140     104
 
Bme1580I G_kGCm'C

Cuts at:      0     229     459 
   Size:        229     230
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     451     459 
   Size:         94     357       8

  Fragments arranged by size: 

                 357      94       8
 
Bpu10I CC'TnA_GC

Cuts at:      0      44     459 
   Size:         44     415
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     459 
   Size:        205     254
 
BsaHI Gr'CG_yC

Cuts at:      0       4     459 
   Size:          4     455
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     265     268     271     397     459 
   Size:        265       3       3     126      62

  Fragments arranged by size: 

                 265     126      62       3       3
 
BsiHKAI G_wGCw'C

Cuts at:      0      80     221     389     459 
   Size:         80     141     168      70

  Fragments arranged by size: 

                 168     141      80      70
 
BsmI GAATG_Cn'

Cuts at:      0     305     459 
   Size:        305     154
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      46     459 
   Size:         46     413
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     221     229     389     459 
   Size:         80     141       8     160      70

  Fragments arranged by size: 

                 160     141      80      70       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     155     459 
   Size:        155     304
 
BsrGI T'GTAC_A

Cuts at:      0     452     459 
   Size:        452       7
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     171     459 
   Size:        171     288
 
BtgI C'CryG_G

Cuts at:      0     441     459 
   Size:        441      18
 
EcoICRI GAG'CTC

Cuts at:      0      78     219     459 
   Size:         78     141     240

  Fragments arranged by size: 

                 240     141      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     451     459 
   Size:         94     357       8

  Fragments arranged by size: 

                 357      94       8
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      17     459 
   Size:         17     442
 
MslI CAynn'nnrTG

Cuts at:      0     271     459 
   Size:        271     188
 
MspA1I CmG'CkG

Cuts at:      0     160     302     459 
   Size:        160     142     157

  Fragments arranged by size: 

                 160     157     142
 
NcoI C'CATG_G

Cuts at:      0     441     459 
   Size:        441      18
 
NspI r_CATG'y

Cuts at:      0      69     459 
   Size:         69     390
 
PvuII CAG'CTG

Cuts at:      0     160     302     459 
   Size:        160     142     157

  Fragments arranged by size: 

                 160     157     142
 
SacI G_AGCT'C

Cuts at:      0      80     221     459 
   Size:         80     141     238

  Fragments arranged by size: 

                 238     141      80
 
SmlI C'TyrA_G

Cuts at:      0     220     459 
   Size:        220     239
 
StyI C'CwwG_G

Cuts at:      0     441     459 
   Size:        441      18
 
TatI w'GTAC_w

Cuts at:      0     452     459 
   Size:        452       7
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      69     459 
   Size:         69     390
 

 Enzymes that do cut:

     AarI     ApoI    BanII     BbsI     BlpI Bme1580I     BpmI   Bpu10I
    BpuEI    BsaHI    BseRI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI
    BsrGI    BstXI     BtgI  EcoICRI  Eco57MI     MmeI     MslI   MspA1I
     NcoI     NspI    PvuII     SacI     SmlI     StyI     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI