Restriction map for
pFN21AE3969
(Linear) (Six-base) MAPSORT of: KIEE3969.seq Check: 9870 from: 1 to: 1254
KIEE3969.orf
With 182 enzymes: SgfI *
March 22, 2012 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 78 167 1254
Size: 78 89 1087
Fragments arranged by size:
1087 89 78
AatII G_ACGT'C
Cuts at: 0 65 1254
Size: 65 1189
AflIII A'CryG_T
Cuts at: 0 329 1254
Size: 329 925
AleI CACnn'nnGTG
Cuts at: 0 950 1254
Size: 950 304
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 844 876 1254
Size: 844 32 378
Fragments arranged by size:
844 378 32
AlwNI CAG_nnn'CTG
Cuts at: 0 225 296 710 888 909 1254
Size: 225 71 414 178 21 345
Fragments arranged by size:
414 345 225 178 71 21
ApaLI G'TGCA_C
Cuts at: 0 93 1254
Size: 93 1161
AvaI C'yCGr_G
Cuts at: 0 200 671 843 1235 1254
Size: 200 471 172 392 19
Fragments arranged by size:
471 392 200 172 19
BanI G'GyrC_C
Cuts at: 0 563 1254
Size: 563 691
BbvCI CC'TCA_GC
Cuts at: 0 244 289 703 1254
Size: 244 45 414 551
Fragments arranged by size:
551 414 244 45
BfrBI ATG'CAT
Cuts at: 0 816 1254
Size: 816 438
BglII A'GATC_T
Cuts at: 0 648 1254
Size: 648 606
BlpI GC'TnA_GC
Cuts at: 0 1055 1254
Size: 1055 199
Bme1580I G_kGCm'C
Cuts at: 0 97 566 1254
Size: 97 469 688
Fragments arranged by size:
688 469 97
BmrI ACTGGGnnnn_n'
Cuts at: 0 411 1254
Size: 411 843
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 366 1254
Size: 366 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 463 631 1063 1141 1230 1254
Size: 454 9 168 432 78 89 24
Fragments arranged by size:
454 432 168 89 78 24 9
Bpu10I CC'TnA_GC
Cuts at: 0 226 244 289 370 703 1063 1254
Size: 226 18 45 81 333 360 191
Fragments arranged by size:
360 333 226 191 81 45 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 496 708 904 1132 1254
Size: 409 87 212 196 228 122
Fragments arranged by size:
409 228 212 196 122 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 872 1254
Size: 872 382
BsaAI yAC'GTr
Cuts at: 0 252 1254
Size: 252 1002
BsaHI Gr'CG_yC
Cuts at: 0 62 110 1254
Size: 62 48 1144
Fragments arranged by size:
1144 62 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 766 796 1254
Size: 766 30 458
Fragments arranged by size:
766 458 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 1254
Size: 685 569
BseYI C'CCAG_C
Cuts at: 0 187 321 447 1254
Size: 187 134 126 807
Fragments arranged by size:
807 187 134 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 238 358 724 1080 1254
Size: 139 99 120 366 356 174
Fragments arranged by size:
366 356 174 139 120 99
BsiHKAI G_wGCw'C
Cuts at: 0 97 1061 1254
Size: 97 964 193
Fragments arranged by size:
964 193 97
Bsp1286I G_dGCh'C
Cuts at: 0 97 566 1061 1254
Size: 97 469 495 193
Fragments arranged by size:
495 469 193 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 167 785 1254
Size: 78 89 618 469
Fragments arranged by size:
618 469 89 78
BsrFI r'CCGG_y
Cuts at: 0 571 1075 1254
Size: 571 504 179
Fragments arranged by size:
571 504 179
BssHII G'CGCG_C
Cuts at: 0 78 357 1254
Size: 78 279 897
Fragments arranged by size:
897 279 78
BssSI C'ACGA_G
Cuts at: 0 1199 1254
Size: 1199 55
BstAPI GCAn_nnn'nTGC
Cuts at: 0 993 1254
Size: 993 261
BstYI r'GATC_y
Cuts at: 0 221 648 1254
Size: 221 427 606
Fragments arranged by size:
606 427 221
BtgI C'CryG_G
Cuts at: 0 824 1254
Size: 824 430
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 474 1254
Size: 474 780
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 33 1254
Size: 33 1221
EaeI y'GGCC_r
Cuts at: 0 38 266 569 821 1254
Size: 38 228 303 252 433
Fragments arranged by size:
433 303 252 228 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 199 223 607 1254
Size: 199 24 384 647
Fragments arranged by size:
647 384 199 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 463 631 1063 1141 1230 1254
Size: 454 9 168 432 78 89 24
Fragments arranged by size:
454 432 168 89 78 24 9
EcoO109I rG'GnC_Cy
Cuts at: 0 429 1038 1254
Size: 429 609 216
Fragments arranged by size:
609 429 216
FspI TGC'GCA
Cuts at: 0 766 997 1024 1254
Size: 766 231 27 230
Fragments arranged by size:
766 231 230 27
FspAI rTGC'GCAy
Cuts at: 0 1024 1254
Size: 1024 230
HaeII r_GCGC'y
Cuts at: 0 510 1254
Size: 510 744
MluI A'CGCG_T
Cuts at: 0 329 1254
Size: 329 925
MscI TGG'CCA
Cuts at: 0 40 268 823 1254
Size: 40 228 555 431
Fragments arranged by size:
555 431 228 40
MslI CAynn'nnrTG
Cuts at: 0 950 1254
Size: 950 304
MspA1I CmG'CkG
Cuts at: 0 293 533 1137 1254
Size: 293 240 604 117
Fragments arranged by size:
604 293 240 117
NaeI GCC'GGC
Cuts at: 0 573 1254
Size: 573 681
NcoI C'CATG_G
Cuts at: 0 824 1254
Size: 824 430
NgoMIV G'CCGG_C
Cuts at: 0 571 1254
Size: 571 683
NsiI A_TGCA'T
Cuts at: 0 818 1254
Size: 818 436
PasI CC'CwG_GG
Cuts at: 0 903 1254
Size: 903 351
PflMI CCAn_nnn'nTGG
Cuts at: 0 264 888 909 1254
Size: 264 624 21 345
Fragments arranged by size:
624 345 264 21
PfoI T'CCnGG_A
Cuts at: 0 26 65 1254
Size: 26 39 1189
Fragments arranged by size:
1189 39 26
PshAI GACnn'nnGTC
Cuts at: 0 884 1254
Size: 884 370
PstI C_TGCA'G
Cuts at: 0 197 506 1254
Size: 197 309 748
Fragments arranged by size:
748 309 197
PvuII CAG'CTG
Cuts at: 0 533 1137 1254
Size: 533 604 117
Fragments arranged by size:
604 533 117
SbfI CC_TGCA'GG
Cuts at: 0 197 1254
Size: 197 1057
SfcI C'TryA_G
Cuts at: 0 193 502 625 1254
Size: 193 309 123 629
Fragments arranged by size:
629 309 193 123
SmaI CCC'GGG
Cuts at: 0 202 1254
Size: 202 1052
SmlI C'TyrA_G
Cuts at: 0 424 511 687 919 1147 1254
Size: 424 87 176 232 228 107
Fragments arranged by size:
424 232 228 176 107 87
StyI C'CwwG_G
Cuts at: 0 824 1254
Size: 824 430
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1004 1036 1254
Size: 1004 32 218
Fragments arranged by size:
1004 218 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 910 1116 1254
Size: 910 206 138
Fragments arranged by size:
910 206 138
XmaI C'CCGG_G
Cuts at: 0 200 1254
Size: 200 1054
ZraI GAC'GTC
Cuts at: 0 63 1254
Size: 63 1191
Enzymes that do cut:
AarI AatII AflIII AleI AloI AlwNI ApaLI AvaI
BanI BbvCI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI
BstAPI BstYI BtgI BtgZI CspCI EaeI EciI Eco57MI
EcoO109I FspI FspAI HaeII MluI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI PasI PflMI PfoI PshAI
PstI PvuII SbfI SfcI SmaI SmlI StyI TstI
XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbsI BcgI BciVI BclI BglI
BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BstBI BstEII BstXI
BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI EagI
EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MmeI NarI
NdeI NheI NotI NruI NspI PacI PciI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI
XmnI