Restriction map for
pFN21AE3967
(Linear) (Six-base) MAPSORT of: KIEE3967.seq Check: 5880 from: 1 to: 1212
KIEE3967.orf
With 182 enzymes: SgfI *
March 22, 2012 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 596 1212
Size: 596 616
AatII G_ACGT'C
Cuts at: 0 872 1212
Size: 872 340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 1126 1212
Size: 279 847 86
Fragments arranged by size:
847 279 86
AflIII A'CryG_T
Cuts at: 0 686 1212
Size: 686 526
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 163 195 1212
Size: 163 32 1017
Fragments arranged by size:
1017 163 32
AlwNI CAG_nnn'CTG
Cuts at: 0 956 1212
Size: 956 256
ApaI G_GGCC'C
Cuts at: 0 963 1212
Size: 963 249
AvaI C'yCGr_G
Cuts at: 0 43 1212
Size: 43 1169
AvrII C'CTAG_G
Cuts at: 0 1087 1212
Size: 1087 125
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 770 803 1212
Size: 770 33 409
Fragments arranged by size:
770 409 33
BanI G'GyrC_C
Cuts at: 0 47 1212
Size: 47 1165
BanII G_rGCy'C
Cuts at: 0 431 963 1212
Size: 431 532 249
Fragments arranged by size:
532 431 249
BbeI G_GCGC'C
Cuts at: 0 51 1212
Size: 51 1161
BbsI GAAGACnn'nnnn_
Cuts at: 0 262 1212
Size: 262 950
BbvCI CC'TCA_GC
Cuts at: 0 820 1212
Size: 820 392
BglI GCCn_nnn'nGGC
Cuts at: 0 931 1212
Size: 931 281
BglII A'GATC_T
Cuts at: 0 407 471 889 1212
Size: 407 64 418 323
Fragments arranged by size:
418 407 323 64
Bme1580I G_kGCm'C
Cuts at: 0 336 356 963 1212
Size: 336 20 607 249
Fragments arranged by size:
607 336 249 20
BmgBI CAC'GTC
Cuts at: 0 1068 1212
Size: 1068 144
BmrI ACTGGGnnnn_n'
Cuts at: 0 482 1212
Size: 482 730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 668 890 1212
Size: 108 560 222 322
Fragments arranged by size:
560 322 222 108
Bpu10I CC'TnA_GC
Cuts at: 0 820 1212
Size: 820 392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 116 1212
Size: 22 94 1096
Fragments arranged by size:
1096 94 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 134 513 1212
Size: 134 379 699
Fragments arranged by size:
699 379 134
BsaBI GATnn'nnATC
Cuts at: 0 406 1212
Size: 406 806
BsaHI Gr'CG_yC
Cuts at: 0 48 869 1212
Size: 48 821 343
Fragments arranged by size:
821 343 48
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 163 193 1212
Size: 163 30 1019
Fragments arranged by size:
1019 163 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 937 1212
Size: 187 750 275
Fragments arranged by size:
750 275 187
BseYI C'CCAG_C
Cuts at: 0 316 1212
Size: 316 896
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 999 1212
Size: 439 560 213
Fragments arranged by size:
560 439 213
BsiHKAI G_wGCw'C
Cuts at: 0 640 1212
Size: 640 572
BsmBI CGTCTCn'nnnn_
Cuts at: 0 220 1212
Size: 220 992
Bsp1286I G_dGCh'C
Cuts at: 0 336 356 431 640 963 1212
Size: 336 20 75 209 323 249
Fragments arranged by size:
336 323 249 209 75 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 596 1212
Size: 596 616
BsrDI GCAATG_nn'
Cuts at: 0 980 1040 1212
Size: 980 60 172
Fragments arranged by size:
980 172 60
BsrFI r'CCGG_y
Cuts at: 0 808 1212
Size: 808 404
BstAPI GCAn_nnn'nTGC
Cuts at: 0 635 1212
Size: 635 577
BstBI TT'CG_AA
Cuts at: 0 1131 1212
Size: 1131 81
BstYI r'GATC_y
Cuts at: 0 407 471 889 953 1212
Size: 407 64 418 64 259
Fragments arranged by size:
418 407 259 64 64
Bsu36I CC'TnA_GG
Cuts at: 0 949 1212
Size: 949 263
BtgI C'CryG_G
Cuts at: 0 301 854 926 1212
Size: 301 553 72 286
Fragments arranged by size:
553 301 286 72
BtsI GCAGTG_nn'
Cuts at: 0 138 598 726 1162 1212
Size: 138 460 128 436 50
Fragments arranged by size:
460 436 138 128 50
DraIII CAC_nnn'GTG
Cuts at: 0 490 1033 1212
Size: 490 543 179
Fragments arranged by size:
543 490 179
DrdI GACnn_nn'nnGTC
Cuts at: 0 868 1212
Size: 868 344
EaeI y'GGCC_r
Cuts at: 0 29 163 450 1062 1212
Size: 29 134 287 612 150
Fragments arranged by size:
612 287 150 134 29
EarI CTCTTCn'nnn_
Cuts at: 0 536 1212
Size: 536 676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 279 668 890 1126 1212
Size: 108 171 389 222 236 86
Fragments arranged by size:
389 236 222 171 108 86
EcoO109I rG'GnC_Cy
Cuts at: 0 543 680 798 960 1092 1212
Size: 543 137 118 162 132 120
Fragments arranged by size:
543 162 137 132 120 118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 678 1212
Size: 678 534
HaeII r_GCGC'y
Cuts at: 0 51 1212
Size: 51 1161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 128 160 1212
Size: 55 32 41 32 1052
Fragments arranged by size:
1052 55 41 32 32
HincII GTy'rAC
Cuts at: 0 846 1212
Size: 846 366
KasI G'GCGC_C
Cuts at: 0 47 1212
Size: 47 1165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 1212
Size: 149 1063
MscI TGG'CCA
Cuts at: 0 1064 1212
Size: 1064 148
MslI CAynn'nnrTG
Cuts at: 0 458 1212
Size: 458 754
MspA1I CmG'CkG
Cuts at: 0 21 77 646 1212
Size: 21 56 569 566
Fragments arranged by size:
569 566 56 21
NarI GG'CG_CC
Cuts at: 0 48 1212
Size: 48 1164
NcoI C'CATG_G
Cuts at: 0 854 1212
Size: 854 358
NspI r_CATG'y
Cuts at: 0 633 1212
Size: 633 579
PflMI CCAn_nnn'nTGG
Cuts at: 0 1033 1212
Size: 1033 179
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 1212
Size: 163 32 1017
Fragments arranged by size:
1017 163 32
PpuMI rG'GwC_Cy
Cuts at: 0 543 680 798 1092 1212
Size: 543 137 118 294 120
Fragments arranged by size:
543 294 137 120 118
PshAI GACnn'nnGTC
Cuts at: 0 269 1212
Size: 269 943
PspOMI G'GGCC_C
Cuts at: 0 959 1212
Size: 959 253
PstI C_TGCA'G
Cuts at: 0 593 628 645 1212
Size: 593 35 17 567
Fragments arranged by size:
593 567 35 17
PvuII CAG'CTG
Cuts at: 0 77 646 1212
Size: 77 569 566
Fragments arranged by size:
569 566 77
SanDI GG'GwC_CC
Cuts at: 0 798 1212
Size: 798 414
SfcI C'TryA_G
Cuts at: 0 589 624 641 1212
Size: 589 35 17 571
Fragments arranged by size:
589 571 35 17
SfoI GGC'GCC
Cuts at: 0 49 1212
Size: 49 1163
SgrAI Cr'CCGG_yG
Cuts at: 0 808 1212
Size: 808 404
SmaI CCC'GGG
Cuts at: 0 45 1212
Size: 45 1167
SmlI C'TyrA_G
Cuts at: 0 37 131 1212
Size: 37 94 1081
Fragments arranged by size:
1081 94 37
SphI G_CATG'C
Cuts at: 0 633 1212
Size: 633 579
SrfI GCCC'GGGC
Cuts at: 0 45 1212
Size: 45 1167
StyI C'CwwG_G
Cuts at: 0 854 1087 1212
Size: 854 233 125
Fragments arranged by size:
854 233 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 330 501 648 853 1212
Size: 330 171 147 205 359
Fragments arranged by size:
359 330 205 171 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1008 1040 1154 1186 1212
Size: 1008 32 114 32 26
Fragments arranged by size:
1008 114 32 32 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 113 1212
Size: 113 1099
XmaI C'CCGG_G
Cuts at: 0 43 1212
Size: 43 1169
XmnI GAAnn'nnTTC
Cuts at: 0 1189 1212
Size: 1189 23
ZraI GAC'GTC
Cuts at: 0 870 1212
Size: 870 342
Enzymes that do cut:
AarI AatII AcuI AflIII AloI AlwNI ApaI AvaI
AvrII BaeI BanI BanII BbeI BbsI BbvCI BglI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrDI BsrFI BstAPI BstBI BstYI Bsu36I
BtgI BtsI DraIII DrdI EaeI EarI Eco57MI EcoO109I
FalI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NarI NcoI NspI PflMI PpiI PpuMI PshAI
PspOMI PstI PvuII SanDI SfcI SfoI SgrAI SmaI
SmlI SphI SrfI StyI TaqII TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI BamHI BcgI
BciVI BclI BfrBI BlpI BmtI BplI BsaAI BsaWI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SgfI SnaBI SpeI SspI StuI SwaI TatI Tth111I
XbaI XhoI