Restriction map for
pFN21AE3965
(Linear) (Six-base) MAPSORT of: KIEE3965.seq Check: 5326 from: 1 to: 996
KIEE3965.orf
With 182 enzymes: SgfI *
March 22, 2012 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 336 713 996
Size: 212 124 377 283
Fragments arranged by size:
377 283 212 124
AfeI AGC'GCT
Cuts at: 0 524 996
Size: 524 472
AflII C'TTAA_G
Cuts at: 0 673 996
Size: 673 323
ApoI r'AATT_y
Cuts at: 0 135 557 996
Size: 135 422 439
Fragments arranged by size:
439 422 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 488 994 996
Size: 488 506 2
Fragments arranged by size:
506 488 2
BglII A'GATC_T
Cuts at: 0 502 996
Size: 502 494
Bme1580I G_kGCm'C
Cuts at: 0 916 996
Size: 916 80
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 306 858 996
Size: 144 162 552 138
Fragments arranged by size:
552 162 144 138
Bpu10I CC'TnA_GC
Cuts at: 0 534 996
Size: 534 462
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 996
Size: 638 358
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 996
Size: 139 857
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 942 996
Size: 942 54
Bsp1286I G_dGCh'C
Cuts at: 0 916 996
Size: 916 80
BspHI T'CATG_A
Cuts at: 0 666 996
Size: 666 330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 76 996
Size: 76 920
BsrDI GCAATG_nn'
Cuts at: 0 312 996
Size: 312 684
BsrFI r'CCGG_y
Cuts at: 0 85 996
Size: 85 911
BstXI CCAn_nnnn'nTGG
Cuts at: 0 90 996
Size: 90 906
BstYI r'GATC_y
Cuts at: 0 502 996
Size: 502 494
BtgI C'CryG_G
Cuts at: 0 575 996
Size: 575 421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 36 996
Size: 36 960
BtsI GCAGTG_nn'
Cuts at: 0 916 996
Size: 916 80
EarI CTCTTCn'nnn_
Cuts at: 0 137 732 797 996
Size: 137 595 65 199
Fragments arranged by size:
595 199 137 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 336 713 996
Size: 212 124 377 283
Fragments arranged by size:
377 283 212 124
HaeII r_GCGC'y
Cuts at: 0 526 996
Size: 526 470
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 204 236 996
Size: 204 32 760
Fragments arranged by size:
760 204 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 996
Size: 740 256
MslI CAynn'nnrTG
Cuts at: 0 55 879 996
Size: 55 824 117
Fragments arranged by size:
824 117 55
NcoI C'CATG_G
Cuts at: 0 575 996
Size: 575 421
NspI r_CATG'y
Cuts at: 0 293 343 878 996
Size: 293 50 535 118
Fragments arranged by size:
535 293 118 50
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 996
Size: 136 32 828
Fragments arranged by size:
828 136 32
PsiI TTA'TAA
Cuts at: 0 611 996
Size: 611 385
PstI C_TGCA'G
Cuts at: 0 335 625 996
Size: 335 290 371
Fragments arranged by size:
371 335 290
SfcI C'TryA_G
Cuts at: 0 331 621 996
Size: 331 290 375
Fragments arranged by size:
375 331 290
SmlI C'TyrA_G
Cuts at: 0 653 673 996
Size: 653 20 323
Fragments arranged by size:
653 323 20
SspI AAT'ATT
Cuts at: 0 629 640 996
Size: 629 11 356
Fragments arranged by size:
629 356 11
StyI C'CwwG_G
Cuts at: 0 575 996
Size: 575 421
Enzymes that do cut:
AcuI AfeI AflII ApoI BbsI BglII Bme1580I BmrI
Bpu10I BpuEI BsaI BsgI Bsp1286I BspHI BspMI BsrDI
BsrFI BstXI BstYI BtgI BtgZI BtsI EarI Eco57MI
HaeII Hin4I MmeI MslI NcoI NspI PpiI PsiI
PstI SfcI SmlI SspI StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BplI BpmI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI