Restriction map for
pFN21AE3962
(Linear) (Six-base) MAPSORT of: KIEE3962.seq Check: 71 from: 1 to: 1533
KIEE3962.orf
With 182 enzymes: SgfI *
March 22, 2012 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 41 291 444 473 567 1533
Size: 41 250 153 29 94 966
Fragments arranged by size:
966 250 153 94 41 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1533
Size: 1022 511
AfeI AGC'GCT
Cuts at: 0 1399 1533
Size: 1399 134
AflIII A'CryG_T
Cuts at: 0 1262 1533
Size: 1262 271
AleI CACnn'nnGTG
Cuts at: 0 95 415 494 1007 1363 1533
Size: 95 320 79 513 356 170
Fragments arranged by size:
513 356 320 170 95 79
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 512 1533
Size: 512 1021
AlwNI CAG_nnn'CTG
Cuts at: 0 16 1060 1192 1220 1533
Size: 16 1044 132 28 313
Fragments arranged by size:
1044 313 132 28 16
ApaLI G'TGCA_C
Cuts at: 0 1426 1533
Size: 1426 107
AvaI C'yCGr_G
Cuts at: 0 374 381 483 1096 1275 1447 1533
Size: 374 7 102 613 179 172 86
Fragments arranged by size:
613 374 179 172 102 86 7
BamHI G'GATC_C
Cuts at: 0 946 1533
Size: 946 587
BanI G'GyrC_C
Cuts at: 0 222 385 488 696 799 1009 1168 1533
Size: 222 163 103 208 103 210 159 365
Fragments arranged by size:
365 222 210 208 163 159 103 103
BanII G_rGCy'C
Cuts at: 0 381 767 1417 1533
Size: 381 386 650 116
Fragments arranged by size:
650 386 381 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 973 1473 1533
Size: 973 500 60
Fragments arranged by size:
973 500 60
BbvCI CC'TCA_GC
Cuts at: 0 569 836 1533
Size: 569 267 697
Fragments arranged by size:
697 569 267
BciVI GTATCCnnnnn_n'
Cuts at: 0 873 1533
Size: 873 660
BlpI GC'TnA_GC
Cuts at: 0 1193 1533
Size: 1193 340
Bme1580I G_kGCm'C
Cuts at: 0 138 227 388 422 491 1014 1430 1533
Size: 138 89 161 34 69 523 416 103
Fragments arranged by size:
523 416 161 138 103 89 69 34
BmgBI CAC'GTC
Cuts at: 0 102 478 1533
Size: 102 376 1055
Fragments arranged by size:
1055 376 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 1103 1533
Size: 1103 430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1075 1522 1533
Size: 1075 447 11
Fragments arranged by size:
1075 447 11
Bpu10I CC'TnA_GC
Cuts at: 0 569 836 1533
Size: 569 267 697
Fragments arranged by size:
697 569 267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1533
Size: 79 1454
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 643 907 1533
Size: 55 588 264 626
Fragments arranged by size:
626 588 264 55
BsaAI yAC'GTr
Cuts at: 0 1205 1533
Size: 1205 328
BsaWI w'CCGG_w
Cuts at: 0 216 1533
Size: 216 1317
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 775 1533
Size: 775 758
BseYI C'CCAG_C
Cuts at: 0 826 1119 1533
Size: 826 293 414
Fragments arranged by size:
826 414 293
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 485 755 1233 1533
Size: 458 27 270 478 300
Fragments arranged by size:
478 458 300 270 27
BsiEI CG_ry'CG
Cuts at: 0 158 314 1533
Size: 158 156 1219
Fragments arranged by size:
1219 158 156
BsiHKAI G_wGCw'C
Cuts at: 0 308 1417 1430 1533
Size: 308 1109 13 103
Fragments arranged by size:
1109 308 103 13
BsmI GAATG_Cn'
Cuts at: 0 113 355 1533
Size: 113 242 1178
Fragments arranged by size:
1178 242 113
Bsp1286I G_dGCh'C
Cuts at: 0 138 227 308 381 388 422 491 767
Size: 138 89 81 73 7 34 69 276
Cuts at: 767 1014 1417 1430 1533
Size: 247 403 13 103
Fragments arranged by size:
403 276 247 138 103 89 81 73
69 34 13 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 291 444 473 567 1343 1533
Size: 41 250 153 29 94 776 190
Fragments arranged by size:
776 250 190 153 94 41 29
BsrFI r'CCGG_y
Cuts at: 0 157 1360 1533
Size: 157 1203 173
Fragments arranged by size:
1203 173 157
BsrGI T'GTAC_A
Cuts at: 0 260 1533
Size: 260 1273
BssHII G'CGCG_C
Cuts at: 0 1368 1452 1454 1493 1533
Size: 1368 84 2 39 40
Fragments arranged by size:
1368 84 40 39 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 1533
Size: 237 1296
BstYI r'GATC_y
Cuts at: 0 203 946 1533
Size: 203 743 587
Fragments arranged by size:
743 587 203
Bsu36I CC'TnA_GG
Cuts at: 0 1164 1533
Size: 1164 369
BtgI C'CryG_G
Cuts at: 0 599 1154 1533
Size: 599 555 379
Fragments arranged by size:
599 555 379
BtsI GCAGTG_nn'
Cuts at: 0 734 1533
Size: 734 799
EaeI y'GGCC_r
Cuts at: 0 155 192 311 1533
Size: 155 37 119 1222
Fragments arranged by size:
1222 155 119 37
EagI C'GGCC_G
Cuts at: 0 155 311 1533
Size: 155 156 1222
Fragments arranged by size:
1222 156 155
EarI CTCTTCn'nnn_
Cuts at: 0 997 1405 1533
Size: 997 408 128
Fragments arranged by size:
997 408 128
EcoICRI GAG'CTC
Cuts at: 0 1415 1533
Size: 1415 118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 1075 1522 1533
Size: 1022 53 447 11
Fragments arranged by size:
1022 447 53 11
EcoO109I rG'GnC_Cy
Cuts at: 0 574 1128 1533
Size: 574 554 405
Fragments arranged by size:
574 554 405
FspI TGC'GCA
Cuts at: 0 112 440 1435 1533
Size: 112 328 995 98
Fragments arranged by size:
995 328 112 98
FspAI rTGC'GCAy
Cuts at: 0 112 1435 1533
Size: 112 1323 98
Fragments arranged by size:
1323 112 98
HaeII r_GCGC'y
Cuts at: 0 522 550 890 1401 1509 1533
Size: 522 28 340 511 108 24
Fragments arranged by size:
522 511 340 108 28 24
HindIII A'AGCT_T
Cuts at: 0 122 1533
Size: 122 1411
MslI CAynn'nnrTG
Cuts at: 0 95 415 494 1007 1363 1533
Size: 95 320 79 513 356 170
Fragments arranged by size:
513 356 320 170 95 79
MspA1I CmG'CkG
Cuts at: 0 143 246 266 444 540 601 835 1119
Size: 143 103 20 178 96 61 234 284
Cuts at: 1119 1533
Size: 414
Fragments arranged by size:
414 284 234 178 143 103 96 61
20
NaeI GCC'GGC
Cuts at: 0 159 1533
Size: 159 1374
NgoMIV G'CCGG_C
Cuts at: 0 157 1533
Size: 157 1376
NspI r_CATG'y
Cuts at: 0 1266 1434 1533
Size: 1266 168 99
Fragments arranged by size:
1266 168 99
PasI CC'CwG_GG
Cuts at: 0 973 1015 1533
Size: 973 42 518
Fragments arranged by size:
973 518 42
PciI A'CATG_T
Cuts at: 0 1262 1533
Size: 1262 271
PflMI CCAn_nnn'nTGG
Cuts at: 0 468 1533
Size: 468 1065
PfoI T'CCnGG_A
Cuts at: 0 1240 1533
Size: 1240 293
PpuMI rG'GwC_Cy
Cuts at: 0 1128 1533
Size: 1128 405
PshAI GACnn'nnGTC
Cuts at: 0 919 1533
Size: 919 614
PspXI vC'TCGA_Gb
Cuts at: 0 374 1533
Size: 374 1159
PstI C_TGCA'G
Cuts at: 0 12 38 302 1533
Size: 12 26 264 1231
Fragments arranged by size:
1231 264 26 12
PvuII CAG'CTG
Cuts at: 0 246 444 540 835 1119 1533
Size: 246 198 96 295 284 414
Fragments arranged by size:
414 295 284 246 198 96
SacI G_AGCT'C
Cuts at: 0 1417 1533
Size: 1417 116
SacII CC_GC'GG
Cuts at: 0 602 1533
Size: 602 931
SapI GCTCTTCn'nnn_
Cuts at: 0 1405 1533
Size: 1405 128
SbfI CC_TGCA'GG
Cuts at: 0 302 1533
Size: 302 1231
ScaI AGT'ACT
Cuts at: 0 1113 1533
Size: 1113 420
SfcI C'TryA_G
Cuts at: 0 8 34 298 792 1533
Size: 8 26 264 494 741
Fragments arranged by size:
741 494 264 26 8
SgrAI Cr'CCGG_yG
Cuts at: 0 1360 1533
Size: 1360 173
SmaI CCC'GGG
Cuts at: 0 383 485 1533
Size: 383 102 1048
Fragments arranged by size:
1048 383 102
SmlI C'TyrA_G
Cuts at: 0 58 374 1533
Size: 58 316 1159
Fragments arranged by size:
1159 316 58
SnaBI TAC'GTA
Cuts at: 0 1205 1533
Size: 1205 328
StyI C'CwwG_G
Cuts at: 0 563 1533
Size: 563 970
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 629 667 789 997 1533
Size: 629 38 122 208 536
Fragments arranged by size:
629 536 208 122 38
TatI w'GTAC_w
Cuts at: 0 260 1111 1533
Size: 260 851 422
Fragments arranged by size:
851 422 260
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 155 187 1533
Size: 155 32 1346
Fragments arranged by size:
1346 155 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1116 1533
Size: 1116 417
XhoI C'TCGA_G
Cuts at: 0 374 1533
Size: 374 1159
XmaI C'CCGG_G
Cuts at: 0 381 483 1533
Size: 381 102 1050
Fragments arranged by size:
1050 381 102
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BsrGI BssHII BstAPI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FspI
FspAI HaeII HindIII MslI MspA1I NaeI NgoMIV NspI
PasI PciI PflMI PfoI PpuMI PshAI PspXI PstI
PvuII SacI SacII SapI SbfI ScaI SfcI SgrAI
SmaI SmlI SnaBI StyI TaqII TatI TstI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
ApaI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BclI BfrBI BglI BglII BmtI BplI BsaBI
BsaHI BsaXI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoNI EcoRI EcoRV FalI
FseI Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PsiI PspOMI PsrI
PvuI RsrII SalI SanDI SexAI SfiI SfoI SgfI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XmnI ZraI