(Linear) (Six-base) MAPSORT of: KIEE3961.seq  Check: 1882  from: 1  to: 624



KIEE3961.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     159     624 
   Size:        159     465
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     416     624 
   Size:        350      66     208

  Fragments arranged by size: 

                 350     208      66
 
AlwNI CAG_nnn'CTG

Cuts at:      0     295     343     624 
   Size:        295      48     281

  Fragments arranged by size: 

                 295     281      48
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     216     485     624 
   Size:        216     269     139

  Fragments arranged by size: 

                 269     216     139
 
BseYI C'CCAG_C

Cuts at:      0     259     624 
   Size:        259     365
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     474     624 
   Size:        379      95     150

  Fragments arranged by size: 

                 379     150      95
 
BsmI GAATG_Cn'

Cuts at:      0     490     624 
   Size:        490     134
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     159     624 
   Size:        159     465
 
BsrFI r'CCGG_y

Cuts at:      0     488     624 
   Size:        488     136
 
BsrGI T'GTAC_A

Cuts at:      0     513     531     624 
   Size:        513      18      93

  Fragments arranged by size: 

                 513      93      18
 
BstYI r'GATC_y

Cuts at:      0     611     624 
   Size:        611      13
 
BtsI GCAGTG_nn'

Cuts at:      0     213     624 
   Size:        213     411
 
EarI CTCTTCn'nnn_

Cuts at:      0     392     624 
   Size:        392     232
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     416     624 
   Size:        350      66     208

  Fragments arranged by size: 

                 350     208      66
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     127     487     624 
   Size:        127     360     137

  Fragments arranged by size: 

                 360     137     127
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     226     258     307     339     624 
   Size:        226      32      49      32     285

  Fragments arranged by size: 

                 285     226      49      32      32
 
MfeI C'AATT_G

Cuts at:      0     310     537     624 
   Size:        310     227      87

  Fragments arranged by size: 

                 310     227      87
 
NspI r_CATG'y

Cuts at:      0      87     624 
   Size:         87     537
 
SapI GCTCTTCn'nnn_

Cuts at:      0     392     624 
   Size:        392     232
 
SfcI C'TryA_G

Cuts at:      0     296     624 
   Size:        296     328
 
SmlI C'TyrA_G

Cuts at:      0       5     624 
   Size:          5     619
 
TatI w'GTAC_w

Cuts at:      0     319     513     531     624 
   Size:        319     194      18      93

  Fragments arranged by size: 

                 319     194      93      18
 
XmnI GAAnn'nnTTC

Cuts at:      0     110     624 
   Size:        110     514
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     BmrI    BseYI     BsgI     BsmI    BspMI
    BsrFI    BsrGI    BstYI     BtsI     EarI  Eco57MI     FalI    Hin4I
     MfeI     NspI     SapI     SfcI     SmlI     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI