Restriction map for
pFN21AE3959
(Linear) (Six-base) MAPSORT of: KIEE3959.seq Check: 6479 from: 1 to: 1521
KIEE3959.orf
With 182 enzymes: SgfI *
March 22, 2012 16:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 43 406 574 1025 1521
Size: 43 363 168 451 496
Fragments arranged by size:
496 451 363 168 43
AflIII A'CryG_T
Cuts at: 0 800 1521
Size: 800 721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 736 1521
Size: 736 785
AlwNI CAG_nnn'CTG
Cuts at: 0 58 190 688 1521
Size: 58 132 498 833
Fragments arranged by size:
833 498 132 58
ApaI G_GGCC'C
Cuts at: 0 48 168 1521
Size: 48 120 1353
Fragments arranged by size:
1353 120 48
AvaI C'yCGr_G
Cuts at: 0 661 1521
Size: 661 860
BanII G_rGCy'C
Cuts at: 0 48 110 168 452 682 1521
Size: 48 62 58 284 230 839
Fragments arranged by size:
839 284 230 62 58 48
BbvCI CC'TCA_GC
Cuts at: 0 59 1521
Size: 59 1462
BciVI GTATCCnnnnn_n'
Cuts at: 0 1039 1521
Size: 1039 482
BglI GCCn_nnn'nGGC
Cuts at: 0 34 481 664 986 1293 1521
Size: 34 447 183 322 307 228
Fragments arranged by size:
447 322 307 228 183 34
Bme1580I G_kGCm'C
Cuts at: 0 48 168 1521
Size: 48 120 1353
Fragments arranged by size:
1353 120 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 245 1112 1521
Size: 245 867 409
Fragments arranged by size:
867 409 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 167 596 1521
Size: 113 54 429 925
Fragments arranged by size:
925 429 113 54
Bpu10I CC'TnA_GC
Cuts at: 0 59 1521
Size: 59 1462
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 64 94 572 602 1521
Size: 64 30 478 30 919
Fragments arranged by size:
919 478 64 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 221 251 254 593 659 1150 1153 1521
Size: 221 30 3 339 66 491 3 368
Fragments arranged by size:
491 368 339 221 66 30 3 3
BseYI C'CCAG_C
Cuts at: 0 161 225 596 644 1274 1521
Size: 161 64 371 48 630 247
Fragments arranged by size:
630 371 247 161 64 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1110 1521
Size: 928 182 411
Fragments arranged by size:
928 411 182
BsiHKAI G_wGCw'C
Cuts at: 0 682 1521
Size: 682 839
BsmI GAATG_Cn'
Cuts at: 0 989 1521
Size: 989 532
Bsp1286I G_dGCh'C
Cuts at: 0 48 110 168 452 682 1521
Size: 48 62 58 284 230 839
Fragments arranged by size:
839 284 230 62 58 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 43 293 406 574 1025 1190 1521
Size: 43 250 113 168 451 165 331
Fragments arranged by size:
451 331 250 168 165 113 43
BsrDI GCAATG_nn'
Cuts at: 0 1187 1521
Size: 1187 334
BssSI C'ACGA_G
Cuts at: 0 31 877 1521
Size: 31 846 644
Fragments arranged by size:
846 644 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 819 1521
Size: 819 702
BstEII G'GTnAC_C
Cuts at: 0 1095 1521
Size: 1095 426
Bsu36I CC'TnA_GG
Cuts at: 0 214 231 1268 1521
Size: 214 17 1037 253
Fragments arranged by size:
1037 253 214 17
BtgI C'CryG_G
Cuts at: 0 564 783 827 1521
Size: 564 219 44 694
Fragments arranged by size:
694 564 219 44
BtsI GCAGTG_nn'
Cuts at: 0 328 720 1521
Size: 328 392 801
Fragments arranged by size:
801 392 328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 912 947 1521
Size: 912 35 574
Fragments arranged by size:
912 574 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 97 1521
Size: 97 1424
EaeI y'GGCC_r
Cuts at: 0 377 1409 1521
Size: 377 1032 112
Fragments arranged by size:
1032 377 112
EarI CTCTTCn'nnn_
Cuts at: 0 1139 1521
Size: 1139 382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 484 616 1521
Size: 484 132 905
Fragments arranged by size:
905 484 132
EcoICRI GAG'CTC
Cuts at: 0 680 1521
Size: 680 841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 167 596 1521
Size: 113 54 429 925
Fragments arranged by size:
925 429 113 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1295 1521
Size: 1295 226
EcoO109I rG'GnC_Cy
Cuts at: 0 44 165 407 424 860 1521
Size: 44 121 242 17 436 661
Fragments arranged by size:
661 436 242 121 44 17
FspI TGC'GCA
Cuts at: 0 440 1521
Size: 440 1081
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 1521
Size: 64 32 1425
Fragments arranged by size:
1425 64 32
MfeI C'AATT_G
Cuts at: 0 999 1521
Size: 999 522
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1521
Size: 349 1172
MscI TGG'CCA
Cuts at: 0 379 1411 1521
Size: 379 1032 110
Fragments arranged by size:
1032 379 110
MslI CAynn'nnrTG
Cuts at: 0 940 1521
Size: 940 581
MspA1I CmG'CkG
Cuts at: 0 275 644 1521
Size: 275 369 877
Fragments arranged by size:
877 369 275
NcoI C'CATG_G
Cuts at: 0 783 827 1521
Size: 783 44 694
Fragments arranged by size:
783 694 44
NdeI CA'TA_TG
Cuts at: 0 793 854 1521
Size: 793 61 667
Fragments arranged by size:
793 667 61
NspI r_CATG'y
Cuts at: 0 151 804 1247 1368 1521
Size: 151 653 443 121 153
Fragments arranged by size:
653 443 153 151 121
PciI A'CATG_T
Cuts at: 0 800 1521
Size: 800 721
PflMI CCAn_nnn'nTGG
Cuts at: 0 1303 1521
Size: 1303 218
PspOMI G'GGCC_C
Cuts at: 0 44 164 1521
Size: 44 120 1357
Fragments arranged by size:
1357 120 44
PstI C_TGCA'G
Cuts at: 0 15 304 417 815 1201 1521
Size: 15 289 113 398 386 320
Fragments arranged by size:
398 386 320 289 113 15
SacI G_AGCT'C
Cuts at: 0 682 1521
Size: 682 839
SbfI CC_TGCA'GG
Cuts at: 0 1201 1521
Size: 1201 320
SfcI C'TryA_G
Cuts at: 0 11 176 300 413 673 811 1197 1521
Size: 11 165 124 113 260 138 386 324
Fragments arranged by size:
386 324 260 165 138 124 113 11
SspI AAT'ATT
Cuts at: 0 1504 1521
Size: 1504 17
StuI AGG'CCT
Cuts at: 0 212 219 1195 1521
Size: 212 7 976 326
Fragments arranged by size:
976 326 212 7
StyI C'CwwG_G
Cuts at: 0 783 827 1521
Size: 783 44 694
Fragments arranged by size:
783 694 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1119 1521
Size: 1119 402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 913 945 1521
Size: 913 32 576
Fragments arranged by size:
913 576 32
Enzymes that do cut:
AarI AflIII AlfI AlwNI ApaI AvaI BanII BbvCI
BciVI BglI Bme1580I BmrI BpmI Bpu10I BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI
BstAPI BstEII Bsu36I BtgI BtsI CspCI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I
MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI
PciI PflMI PspOMI PstI SacI SbfI SfcI SspI
StuI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstBI BstXI BstYI
BstZ17I BtgZI ClaI DraI DraIII EagI EcoRI EcoRV
FalI FseI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI