Restriction map for
pFN21AE3953
(Linear) (Six-base) MAPSORT of: KIEE3953.seq Check: 2352 from: 1 to: 810
KIEE3953.orf
With 182 enzymes: SgfI *
March 22, 2012 16:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 810
Size: 472 338
AlwNI CAG_nnn'CTG
Cuts at: 0 101 219 655 810
Size: 101 118 436 155
Fragments arranged by size:
436 155 118 101
ApoI r'AATT_y
Cuts at: 0 556 810
Size: 556 254
BanII G_rGCy'C
Cuts at: 0 262 698 810
Size: 262 436 112
Fragments arranged by size:
436 262 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 637 810
Size: 637 173
BbvCI CC'TCA_GC
Cuts at: 0 656 810
Size: 656 154
BciVI GTATCCnnnnn_n'
Cuts at: 0 539 810
Size: 539 271
BclI T'GATC_A
Cuts at: 0 291 561 810
Size: 291 270 249
Fragments arranged by size:
291 270 249
BglII A'GATC_T
Cuts at: 0 282 810
Size: 282 528
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 325 717 810
Size: 325 392 93
Fragments arranged by size:
392 325 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 415 810
Size: 76 339 395
Fragments arranged by size:
395 339 76
Bpu10I CC'TnA_GC
Cuts at: 0 656 810
Size: 656 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 226 427 810
Size: 96 130 201 383
Fragments arranged by size:
383 201 130 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 368 810
Size: 368 442
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 238 268 810
Size: 238 30 542
Fragments arranged by size:
542 238 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 478 604 810
Size: 46 432 126 206
Fragments arranged by size:
432 206 126 46
BsiHKAI G_wGCw'C
Cuts at: 0 374 698 810
Size: 374 324 112
Fragments arranged by size:
374 324 112
Bsp1286I G_dGCh'C
Cuts at: 0 262 374 698 810
Size: 262 112 324 112
Fragments arranged by size:
324 262 112 112
BspHI T'CATG_A
Cuts at: 0 764 810
Size: 764 46
BsrBI CCG'CTC
Cuts at: 0 360 810
Size: 360 450
BstEII G'GTnAC_C
Cuts at: 0 36 379 810
Size: 36 343 431
Fragments arranged by size:
431 343 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 231 810
Size: 54 177 579
Fragments arranged by size:
579 177 54
BstYI r'GATC_y
Cuts at: 0 282 810
Size: 282 528
BtgI C'CryG_G
Cuts at: 0 375 810
Size: 375 435
BtsI GCAGTG_nn'
Cuts at: 0 143 810
Size: 143 667
EaeI y'GGCC_r
Cuts at: 0 90 221 785 810
Size: 90 131 564 25
Fragments arranged by size:
564 131 90 25
EarI CTCTTCn'nnn_
Cuts at: 0 271 322 455 494 810
Size: 271 51 133 39 316
Fragments arranged by size:
316 271 133 51 39
EcoICRI GAG'CTC
Cuts at: 0 696 810
Size: 696 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 415 472 810
Size: 76 339 57 338
Fragments arranged by size:
339 338 76 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 810
Size: 397 413
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 683 715 810
Size: 98 32 553 32 95
Fragments arranged by size:
553 98 95 32 32
HincII GTy'rAC
Cuts at: 0 65 731 810
Size: 65 666 79
Fragments arranged by size:
666 79 65
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 810
Size: 697 113
MscI TGG'CCA
Cuts at: 0 92 223 787 810
Size: 92 131 564 23
Fragments arranged by size:
564 131 92 23
MslI CAynn'nnrTG
Cuts at: 0 118 810
Size: 118 692
MspA1I CmG'CkG
Cuts at: 0 164 810
Size: 164 646
PflMI CCAn_nnn'nTGG
Cuts at: 0 268 810
Size: 268 542
PfoI T'CCnGG_A
Cuts at: 0 511 810
Size: 511 299
PstI C_TGCA'G
Cuts at: 0 749 810
Size: 749 61
PvuII CAG'CTG
Cuts at: 0 164 810
Size: 164 646
SacI G_AGCT'C
Cuts at: 0 698 810
Size: 698 112
SfcI C'TryA_G
Cuts at: 0 202 745 810
Size: 202 543 65
Fragments arranged by size:
543 202 65
SmlI C'TyrA_G
Cuts at: 0 75 241 442 810
Size: 75 166 201 368
Fragments arranged by size:
368 201 166 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 771 810
Size: 771 39
XbaI T'CTAG_A
Cuts at: 0 81 810
Size: 81 729
Enzymes that do cut:
AcuI AlwNI ApoI BanII BbsI BbvCI BciVI BclI
BglII BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BsiHKAI Bsp1286I BspHI BsrBI BstEII BstXI BstYI BtgI
BtsI EaeI EarI EcoICRI Eco57MI EcoNI Hin4I HincII
MmeI MscI MslI MspA1I PflMI PfoI PstI PvuII
SacI SfcI SmlI TaqII XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI