Restriction map for
pFN21AE3950
(Linear) (Six-base) MAPSORT of: KIEE3950.seq Check: 4112 from: 1 to: 2349
KIEE3950.orf
With 182 enzymes: SgfI *
March 22, 2012 16:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 140 1900 2349
Size: 140 1760 449
Fragments arranged by size:
1760 449 140
AatII G_ACGT'C
Cuts at: 0 598 683 2349
Size: 598 85 1666
Fragments arranged by size:
1666 598 85
Acc65I G'GTAC_C
Cuts at: 0 563 2349
Size: 563 1786
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 2214 2349
Size: 34 2180 135
Fragments arranged by size:
2180 135 34
AflIII A'CryG_T
Cuts at: 0 904 2349
Size: 904 1445
AgeI A'CCGG_T
Cuts at: 0 1973 2349
Size: 1973 376
AleI CACnn'nnGTG
Cuts at: 0 1231 2349
Size: 1231 1118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 227 556 2349
Size: 227 329 1793
Fragments arranged by size:
1793 329 227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1342 1374 2349
Size: 1342 32 975
Fragments arranged by size:
1342 975 32
AlwNI CAG_nnn'CTG
Cuts at: 0 132 2349
Size: 132 2217
ApaI G_GGCC'C
Cuts at: 0 800 2221 2349
Size: 800 1421 128
Fragments arranged by size:
1421 800 128
ApaLI G'TGCA_C
Cuts at: 0 1948 2349
Size: 1948 401
AvaI C'yCGr_G
Cuts at: 0 155 348 425 544 722 849 910 1748
Size: 155 193 77 119 178 127 61 838
Cuts at: 1748 2349
Size: 601
Fragments arranged by size:
838 601 193 178 155 127 119 77
61
BanI G'GyrC_C
Cuts at: 0 178 390 430 563 805 817 1429 1533
Size: 178 212 40 133 242 12 612 104
Cuts at: 1533 1660 2349
Size: 127 689
Fragments arranged by size:
689 612 242 212 178 133 127 104
40 12
BanII G_rGCy'C
Cuts at: 0 466 800 850 2221 2349
Size: 466 334 50 1371 128
Fragments arranged by size:
1371 466 334 128 50
BbeI G_GCGC'C
Cuts at: 0 182 821 1433 2349
Size: 182 639 612 916
Fragments arranged by size:
916 639 612 182
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 1714 2241 2349
Size: 449 1265 527 108
Fragments arranged by size:
1265 527 449 108
BbvCI CC'TCA_GC
Cuts at: 0 2263 2349
Size: 2263 86
BclI T'GATC_A
Cuts at: 0 1340 1388 2349
Size: 1340 48 961
Fragments arranged by size:
1340 961 48
BglI GCCn_nnn'nGGC
Cuts at: 0 266 521 854 2349
Size: 266 255 333 1495
Fragments arranged by size:
1495 333 266 255
BglII A'GATC_T
Cuts at: 0 1373 2349
Size: 1373 976
BlpI GC'TnA_GC
Cuts at: 0 304 2349
Size: 304 2045
Bme1580I G_kGCm'C
Cuts at: 0 395 478 800 1927 1952 2221 2349
Size: 395 83 322 1127 25 269 128
Fragments arranged by size:
1127 395 322 269 128 83 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 884 1965 2349
Size: 884 1081 384
Fragments arranged by size:
1081 884 384
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 807 2349
Size: 807 1542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 1000 1354 1456 1579 1620 1666 2349
Size: 220 780 354 102 123 41 46 683
Fragments arranged by size:
780 683 354 220 123 102 46 41
Bpu10I CC'TnA_GC
Cuts at: 0 2263 2349
Size: 2263 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 691 2349
Size: 340 351 1658
Fragments arranged by size:
1658 351 340
BsaI GGTCTCn'nnnn_
Cuts at: 0 281 2349
Size: 281 2068
BsaAI yAC'GTr
Cuts at: 0 473 905 1968 2238 2349
Size: 473 432 1063 270 111
Fragments arranged by size:
1063 473 432 270 111
BsaHI Gr'CG_yC
Cuts at: 0 179 595 680 818 1430 2349
Size: 179 416 85 138 612 919
Fragments arranged by size:
919 612 416 179 138 85
BsaWI w'CCGG_w
Cuts at: 0 1312 1973 2349
Size: 1312 661 376
Fragments arranged by size:
1312 661 376
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1342 1372 1690 1720 2092 2122 2349
Size: 1342 30 318 30 372 30 227
Fragments arranged by size:
1342 372 318 227 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 399 432 997 2349
Size: 399 33 565 1352
Fragments arranged by size:
1352 565 399 33
BseYI C'CCAG_C
Cuts at: 0 137 823 1017 1920 2040 2349
Size: 137 686 194 903 120 309
Fragments arranged by size:
903 686 309 194 137 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 257 269 577 772 1272 1554 1948
Size: 127 130 12 308 195 500 282 394
Cuts at: 1948 2278 2302 2349
Size: 330 24 47
Fragments arranged by size:
500 394 330 308 282 195 130 127
47 24 12
BsiEI CG_ry'CG
Cuts at: 0 636 1911 2349
Size: 636 1275 438
Fragments arranged by size:
1275 636 438
BsiHKAI G_wGCw'C
Cuts at: 0 206 466 1952 2349
Size: 206 260 1486 397
Fragments arranged by size:
1486 397 260 206
Bsp1286I G_dGCh'C
Cuts at: 0 206 395 466 478 800 850 1927 1952
Size: 206 189 71 12 322 50 1077 25
Cuts at: 1952 2221 2349
Size: 269 128
Fragments arranged by size:
1077 322 269 206 189 128 71 50
25 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 140 1900 2274 2349
Size: 140 1760 374 75
Fragments arranged by size:
1760 374 140 75
BsrBI CCG'CTC
Cuts at: 0 144 2349
Size: 144 2205
BsrFI r'CCGG_y
Cuts at: 0 536 989 1973 2349
Size: 536 453 984 376
Fragments arranged by size:
984 536 453 376
BsrGI T'GTAC_A
Cuts at: 0 311 2349
Size: 311 2038
BssSI C'ACGA_G
Cuts at: 0 1003 2349
Size: 1003 1346
BstAPI GCAn_nnn'nTGC
Cuts at: 0 132 941 2349
Size: 132 809 1408
Fragments arranged by size:
1408 809 132
BstEII G'GTnAC_C
Cuts at: 0 2286 2349
Size: 2286 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2073 2349
Size: 2073 276
BstYI r'GATC_y
Cuts at: 0 1133 1373 2349
Size: 1133 240 976
Fragments arranged by size:
1133 976 240
BtgI C'CryG_G
Cuts at: 0 386 510 576 605 1628 2153 2349
Size: 386 124 66 29 1023 525 196
Fragments arranged by size:
1023 525 386 196 124 66 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 84 727 2349
Size: 84 643 1622
Fragments arranged by size:
1622 643 84
BtsI GCAGTG_nn'
Cuts at: 0 487 679 1284 1423 2100 2349
Size: 487 192 605 139 677 249
Fragments arranged by size:
677 605 487 249 192 139
DraIII CAC_nnn'GTG
Cuts at: 0 1008 2349
Size: 1008 1341
EaeI y'GGCC_r
Cuts at: 0 614 2009 2329 2349
Size: 614 1395 320 20
Fragments arranged by size:
1395 614 320 20
EarI CTCTTCn'nnn_
Cuts at: 0 2 1773 2349
Size: 2 1771 576
Fragments arranged by size:
1771 576 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 299 2349
Size: 299 2050
EcoICRI GAG'CTC
Cuts at: 0 464 2349
Size: 464 1885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 220 1000 1354 1456 1579 1620 1666
Size: 34 186 780 354 102 123 41 46
Cuts at: 1666 2214 2349
Size: 548 135
Fragments arranged by size:
780 548 354 186 135 123 102 46
41 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 982 2349
Size: 982 1367
EcoO109I rG'GnC_Cy
Cuts at: 0 338 727 2217 2218 2349
Size: 338 389 1490 1 131
Fragments arranged by size:
1490 389 338 131 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 324 2349
Size: 324 2025
HaeII r_GCGC'y
Cuts at: 0 182 821 1433 2349
Size: 182 639 612 916
Fragments arranged by size:
916 639 612 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1828 1860 2349
Size: 1828 32 489
Fragments arranged by size:
1828 489 32
HindIII A'AGCT_T
Cuts at: 0 1757 2349
Size: 1757 592
KasI G'GCGC_C
Cuts at: 0 178 817 1429 2349
Size: 178 639 612 920
Fragments arranged by size:
920 639 612 178
KpnI G_GTAC'C
Cuts at: 0 567 2349
Size: 567 1782
MscI TGG'CCA
Cuts at: 0 616 2011 2349
Size: 616 1395 338
Fragments arranged by size:
1395 616 338
MslI CAynn'nnrTG
Cuts at: 0 103 1231 1519 2071 2349
Size: 103 1128 288 552 278
Fragments arranged by size:
1128 552 288 278 103
MspA1I CmG'CkG
Cuts at: 0 190 253 329 512 561 578 607 627
Size: 190 63 76 183 49 17 29 20
Cuts at: 627 827 1017 1485 1506 1557 1930 2090 2349
Size: 200 190 468 21 51 373 160 259
Fragments arranged by size:
468 373 259 200 190 190 183 160
76 63 51 49 29 21 20 17
NaeI GCC'GGC
Cuts at: 0 991 2349
Size: 991 1358
NarI GG'CG_CC
Cuts at: 0 179 818 1430 2349
Size: 179 639 612 919
Fragments arranged by size:
919 639 612 179
NcoI C'CATG_G
Cuts at: 0 386 2349
Size: 386 1963
NgoMIV G'CCGG_C
Cuts at: 0 989 2349
Size: 989 1360
NspI r_CATG'y
Cuts at: 0 939 2349
Size: 939 1410
PasI CC'CwG_GG
Cuts at: 0 813 2349
Size: 813 1536
PflMI CCAn_nnn'nTGG
Cuts at: 0 1506 2349
Size: 1506 843
PfoI T'CCnGG_A
Cuts at: 0 1136 1643 1676 2349
Size: 1136 507 33 673
Fragments arranged by size:
1136 673 507 33
PmlI CAC'GTG
Cuts at: 0 473 905 1968 2238 2349
Size: 473 432 1063 270 111
Fragments arranged by size:
1063 473 432 270 111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1342 1374 2349
Size: 1342 32 975
Fragments arranged by size:
1342 975 32
PpuMI rG'GwC_Cy
Cuts at: 0 338 727 2349
Size: 338 389 1622
Fragments arranged by size:
1622 389 338
PspOMI G'GGCC_C
Cuts at: 0 796 2217 2349
Size: 796 1421 132
Fragments arranged by size:
1421 796 132
PspXI vC'TCGA_Gb
Cuts at: 0 544 2349
Size: 544 1805
PstI C_TGCA'G
Cuts at: 0 173 603 1493 1556 1808 1897 2349
Size: 173 430 890 63 252 89 452
Fragments arranged by size:
890 452 430 252 173 89 63
PvuII CAG'CTG
Cuts at: 0 253 627 1506 1557 1930 2090 2349
Size: 253 374 879 51 373 160 259
Fragments arranged by size:
879 374 373 259 253 160 51
SacI G_AGCT'C
Cuts at: 0 466 2349
Size: 466 1883
SacII CC_GC'GG
Cuts at: 0 513 579 608 2349
Size: 513 66 29 1741
Fragments arranged by size:
1741 513 66 29
SanDI GG'GwC_CC
Cuts at: 0 727 2349
Size: 727 1622
SapI GCTCTTCn'nnn_
Cuts at: 0 2 2349
Size: 2 2347
ScaI AGT'ACT
Cuts at: 0 1327 2349
Size: 1327 1022
SfcI C'TryA_G
Cuts at: 0 169 599 930 1489 1552 1804 1893 2349
Size: 169 430 331 559 63 252 89 456
Fragments arranged by size:
559 456 430 331 252 169 89 63
SfoI GGC'GCC
Cuts at: 0 180 819 1431 2349
Size: 180 639 612 918
Fragments arranged by size:
918 639 612 180
SmaI CCC'GGG
Cuts at: 0 157 724 851 2349
Size: 157 567 127 1498
Fragments arranged by size:
1498 567 157 127
SmlI C'TyrA_G
Cuts at: 0 355 544 706 2349
Size: 355 189 162 1643
Fragments arranged by size:
1643 355 189 162
StuI AGG'CCT
Cuts at: 0 1060 2059 2349
Size: 1060 999 290
Fragments arranged by size:
1060 999 290
StyI C'CwwG_G
Cuts at: 0 386 1229 1940 2349
Size: 386 843 711 409
Fragments arranged by size:
843 711 409 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1633 2003 2349
Size: 1633 370 346
Fragments arranged by size:
1633 370 346
TatI w'GTAC_w
Cuts at: 0 311 938 1325 2349
Size: 311 627 387 1024
Fragments arranged by size:
1024 627 387 311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1690 1722 2092 2115 2124 2147 2294 2326
Size: 1690 32 370 23 9 23 147 32
Cuts at: 2326 2349
Size: 23
Fragments arranged by size:
1690 370 147 32 32 23 23 23
9
Tth111I GACn'n_nGTC
Cuts at: 0 2245 2349
Size: 2245 104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 744 1317 1503 2019 2349
Size: 744 573 186 516 330
Fragments arranged by size:
744 573 516 330 186
XhoI C'TCGA_G
Cuts at: 0 544 2349
Size: 544 1805
XmaI C'CCGG_G
Cuts at: 0 155 722 849 2349
Size: 155 567 127 1500
Fragments arranged by size:
1500 567 155 127
ZraI GAC'GTC
Cuts at: 0 596 681 2349
Size: 596 85 1668
Fragments arranged by size:
1668 596 85
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI
BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI
KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SacII SanDI SapI ScaI
SfcI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AhdI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BmgBI
BmtI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EcoRI EcoRV FseI FspI FspAI HincII HpaI
MfeI MluI MmeI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PshAI PsiI PsrI PvuI RsrII
SalI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XmnI