Restriction map for
pFN21AE3945
(Linear) (Six-base) MAPSORT of: KIEE3945.seq Check: 1876 from: 1 to: 1818
KIEE3945.orf
With 182 enzymes: SgfI *
March 22, 2012 16:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 106 1818
Size: 106 1712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 361 1139 1514 1532 1709 1748 1818
Size: 40 321 778 375 18 177 39 70
Fragments arranged by size:
778 375 321 177 70 40 39 18
AflIII A'CryG_T
Cuts at: 0 1786 1818
Size: 1786 32
AlwNI CAG_nnn'CTG
Cuts at: 0 534 1818
Size: 534 1284
ApoI r'AATT_y
Cuts at: 0 91 466 1445 1579 1675 1818
Size: 91 375 979 134 96 143
Fragments arranged by size:
979 375 143 134 96 91
AvaI C'yCGr_G
Cuts at: 0 441 1818
Size: 441 1377
BamHI G'GATC_C
Cuts at: 0 237 1818
Size: 237 1581
BanII G_rGCy'C
Cuts at: 0 1600 1818
Size: 1600 218
BbsI GAAGACnn'nnnn_
Cuts at: 0 121 820 1818
Size: 121 699 998
Fragments arranged by size:
998 699 121
BciVI GTATCCnnnnn_n'
Cuts at: 0 673 1818
Size: 673 1145
BglII A'GATC_T
Cuts at: 0 72 1428 1818
Size: 72 1356 390
Fragments arranged by size:
1356 390 72
BlpI GC'TnA_GC
Cuts at: 0 989 1818
Size: 989 829
BmrI ACTGGGnnnn_n'
Cuts at: 0 827 1818
Size: 827 991
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1684 1818
Size: 727 957 134
Fragments arranged by size:
957 727 134
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1457 1487 1818
Size: 1457 30 331
Fragments arranged by size:
1457 331 30
BseYI C'CCAG_C
Cuts at: 0 8 1025 1735 1818
Size: 8 1017 710 83
Fragments arranged by size:
1017 710 83 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 965 1818
Size: 965 853
BsiHKAI G_wGCw'C
Cuts at: 0 1600 1818
Size: 1600 218
BsmI GAATG_Cn'
Cuts at: 0 973 1818
Size: 973 845
Bsp1286I G_dGCh'C
Cuts at: 0 1600 1818
Size: 1600 218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 106 1818
Size: 106 1712
BsrGI T'GTAC_A
Cuts at: 0 486 1381 1818
Size: 486 895 437
Fragments arranged by size:
895 486 437
BstEII G'GTnAC_C
Cuts at: 0 868 1818
Size: 868 950
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1608 1818
Size: 1608 210
BstYI r'GATC_y
Cuts at: 0 72 237 341 1428 1624 1818
Size: 72 165 104 1087 196 194
Fragments arranged by size:
1087 196 194 165 104 72
BtgI C'CryG_G
Cuts at: 0 956 1818
Size: 956 862
BtsI GCAGTG_nn'
Cuts at: 0 602 947 1818
Size: 602 345 871
Fragments arranged by size:
871 602 345
ClaI AT'CG_AT
Cuts at: 0 98 1818
Size: 98 1720
DraI TTT'AAA
Cuts at: 0 1793 1818
Size: 1793 25
EaeI y'GGCC_r
Cuts at: 0 326 1314 1818
Size: 326 988 504
Fragments arranged by size:
988 504 326
EarI CTCTTCn'nnn_
Cuts at: 0 497 1174 1804 1818
Size: 497 677 630 14
Fragments arranged by size:
677 630 497 14
EcoICRI GAG'CTC
Cuts at: 0 1598 1818
Size: 1598 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 361 727 1139 1514 1532 1684 1709
Size: 40 321 366 412 375 18 152 25
Cuts at: 1709 1748 1818
Size: 39 70
Fragments arranged by size:
412 375 366 321 152 70 40 39
25 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 1818
Size: 55 1763
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 149 877 1537 1818
Size: 149 728 660 281
Fragments arranged by size:
728 660 281 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1335 1367 1457 1489 1818
Size: 1335 32 90 32 329
Fragments arranged by size:
1335 329 90 32 32
HindIII A'AGCT_T
Cuts at: 0 1538 1818
Size: 1538 280
MfeI C'AATT_G
Cuts at: 0 1637 1818
Size: 1637 181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1116 1818
Size: 1116 702
MscI TGG'CCA
Cuts at: 0 328 1316 1818
Size: 328 988 502
Fragments arranged by size:
988 502 328
MslI CAynn'nnrTG
Cuts at: 0 1128 1818
Size: 1128 690
MspA1I CmG'CkG
Cuts at: 0 12 1818
Size: 12 1806
NdeI CA'TA_TG
Cuts at: 0 1702 1818
Size: 1702 116
PfoI T'CCnGG_A
Cuts at: 0 77 1818
Size: 77 1741
PshAI GACnn'nnGTC
Cuts at: 0 125 1818
Size: 125 1693
SacI G_AGCT'C
Cuts at: 0 1600 1818
Size: 1600 218
ScaI AGT'ACT
Cuts at: 0 181 1818
Size: 181 1637
SfcI C'TryA_G
Cuts at: 0 1090 1818
Size: 1090 728
SspI AAT'ATT
Cuts at: 0 691 1818
Size: 691 1127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 597 1162 1591 1698 1818
Size: 597 565 429 107 120
Fragments arranged by size:
597 565 429 120 107
TatI w'GTAC_w
Cuts at: 0 179 272 486 1381 1818
Size: 179 93 214 895 437
Fragments arranged by size:
895 437 214 179 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1046 1078 1818
Size: 1046 32 740
Fragments arranged by size:
1046 740 32
XmnI GAAnn'nnTTC
Cuts at: 0 91 1008 1818
Size: 91 917 810
Fragments arranged by size:
917 810 91
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApoI AvaI BamHI BanII
BbsI BciVI BglII BlpI BmrI BpmI BsaXI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrGI BstEII BstXI
BstYI BtgI BtsI ClaI DraI EaeI EarI EcoICRI
Eco57MI EcoNI FalI Hin4I HindIII MfeI MmeI MscI
MslI MspA1I NdeI PfoI PshAI SacI ScaI SfcI
SspI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BclI BfrBI
BglI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI CspCI DraIII DrdI EagI
EciI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI