(Linear) (Six-base) MAPSORT of: KIEE3944.seq  Check: 8813  from: 1  to: 777



KIEE3944.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:55  ..

AclI AA'CG_TT

Cuts at:      0     113     777 
   Size:        113     664
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240     777 
   Size:        240     537
 
AflII C'TTAA_G

Cuts at:      0     667     777 
   Size:        667     110
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      76     108     777 
   Size:         76      32     669

  Fragments arranged by size: 

                 669      76      32
 
ApoI r'AATT_y

Cuts at:      0     343     777 
   Size:        343     434
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     641     777 
   Size:        641     136
 
BclI T'GATC_A

Cuts at:      0      72     777 
   Size:         72     705
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     777 
   Size:        340     437
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     253     777 
   Size:        253     524
 
BsaBI GATnn'nnATC

Cuts at:      0      71     777 
   Size:         71     706
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     241     568     777 
   Size:        241     327     209

  Fragments arranged by size: 

                 327     241     209
 
BsiEI CG_ry'CG

Cuts at:      0     450     777 
   Size:        450     327
 
BspHI T'CATG_A

Cuts at:      0     683     777 
   Size:        683      94
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     770     777 
   Size:        770       7
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      99     134     777 
   Size:         99      35     643

  Fragments arranged by size: 

                 643      99      35
 
DraI TTT'AAA

Cuts at:      0     588     695     777 
   Size:        588     107      82

  Fragments arranged by size: 

                 588     107      82
 
EarI CTCTTCn'nnn_

Cuts at:      0      15     259     777 
   Size:         15     244     518

  Fragments arranged by size: 

                 518     244      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     240     777 
   Size:        240     537
 
EcoRI G'AATT_C

Cuts at:      0     343     777 
   Size:        343     434
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     720     777 
   Size:        720      57
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     452     478     484     510     777 
   Size:        452      26       6      26     267

  Fragments arranged by size: 

                 452     267      26      26       6
 
MfeI C'AATT_G

Cuts at:      0     743     777 
   Size:        743      34
 
MslI CAynn'nnrTG

Cuts at:      0     100     755     777 
   Size:        100     655      22

  Fragments arranged by size: 

                 655     100      22
 
NspI r_CATG'y

Cuts at:      0     265     777 
   Size:        265     512
 
PvuI CG_AT'CG

Cuts at:      0     450     777 
   Size:        450     327
 
SmlI C'TyrA_G

Cuts at:      0     355     667     777 
   Size:        355     312     110

  Fragments arranged by size: 

                 355     312     110
 
StyI C'CwwG_G

Cuts at:      0     656     777 
   Size:        656     121
 
SwaI ATTT'AAAT

Cuts at:      0     588     777 
   Size:        588     189
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     423     777 
   Size:        423     354
 
TatI w'GTAC_w

Cuts at:      0     624     777 
   Size:        624     153
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     534     777 
   Size:        534     243
 
XmnI GAAnn'nnTTC

Cuts at:      0     308     347     777 
   Size:        308      39     430

  Fragments arranged by size: 

                 430     308      39
 

 Enzymes that do cut:

     AclI     AcuI    AflII     AloI     ApoI     BbsI     BclI    BpuEI
     BsaI    BsaBI    BseRI    BsiEI    BspHI    BtgZI    CspCI     DraI
     EarI  Eco57MI    EcoRI     FalI    Hin4I     MfeI     MslI     NspI
     PvuI     SmlI     StyI     SwaI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI