Restriction map for
pFN21AE3943
(Linear) (Six-base) MAPSORT of: KIEE3943.seq Check: 5143 from: 1 to: 1122
KIEE3943.orf
With 182 enzymes: SgfI *
March 22, 2012 16:55 ..
AatII G_ACGT'C
Cuts at: 0 368 1122
Size: 368 754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 924 1122
Size: 924 198
AlwNI CAG_nnn'CTG
Cuts at: 0 92 101 1122
Size: 92 9 1021
Fragments arranged by size:
1021 92 9
ApaLI G'TGCA_C
Cuts at: 0 379 1122
Size: 379 743
ApoI r'AATT_y
Cuts at: 0 229 1122
Size: 229 893
AvaI C'yCGr_G
Cuts at: 0 300 484 550 916 1122
Size: 300 184 66 366 206
Fragments arranged by size:
366 300 206 184 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 498 531 1122
Size: 498 33 591
Fragments arranged by size:
591 498 33
BanI G'GyrC_C
Cuts at: 0 636 1122
Size: 636 486
BanII G_rGCy'C
Cuts at: 0 491 779 1122
Size: 491 288 343
Fragments arranged by size:
491 343 288
BbeI G_GCGC'C
Cuts at: 0 640 1122
Size: 640 482
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 917 951 1122
Size: 917 34 171
Fragments arranged by size:
917 171 34
BglI GCCn_nnn'nGGC
Cuts at: 0 937 1122
Size: 937 185
Bme1580I G_kGCm'C
Cuts at: 0 300 383 685 1122
Size: 300 83 302 437
Fragments arranged by size:
437 302 300 83
BmrI ACTGGGnnnn_n'
Cuts at: 0 865 1122
Size: 865 257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 343 391 580 787 793 979 1122
Size: 32 311 48 189 207 6 186 143
Fragments arranged by size:
311 207 189 186 143 48 32 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1122
Size: 1060 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 62 1122
Size: 62 1060
BsaHI Gr'CG_yC
Cuts at: 0 365 637 1034 1122
Size: 365 272 397 88
Fragments arranged by size:
397 365 272 88
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 976 1122
Size: 43 933 146
Fragments arranged by size:
933 146 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 1122
Size: 577 545
BsiEI CG_ry'CG
Cuts at: 0 941 1122
Size: 941 181
BsiHKAI G_wGCw'C
Cuts at: 0 383 491 551 1122
Size: 383 108 60 571
Fragments arranged by size:
571 383 108 60
Bsp1286I G_dGCh'C
Cuts at: 0 300 383 491 551 685 779 1122
Size: 300 83 108 60 134 94 343
Fragments arranged by size:
343 300 134 108 94 83 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 127 431 1122
Size: 127 304 691
Fragments arranged by size:
691 304 127
BsrBI CCG'CTC
Cuts at: 0 345 1122
Size: 345 777
BssHII G'CGCG_C
Cuts at: 0 472 579 911 924 973 1043 1122
Size: 472 107 332 13 49 70 79
Fragments arranged by size:
472 332 107 79 70 49 13
BssSI C'ACGA_G
Cuts at: 0 1114 1122
Size: 1114 8
BtgI C'CryG_G
Cuts at: 0 330 827 1122
Size: 330 497 295
Fragments arranged by size:
497 330 295
BtsI GCAGTG_nn'
Cuts at: 0 764 1047 1122
Size: 764 283 75
Fragments arranged by size:
764 283 75
DraIII CAC_nnn'GTG
Cuts at: 0 1026 1122
Size: 1026 96
EaeI y'GGCC_r
Cuts at: 0 2 263 398 938 1122
Size: 2 261 135 540 184
Fragments arranged by size:
540 261 184 135 2
EagI C'GGCC_G
Cuts at: 0 938 1122
Size: 938 184
EarI CTCTTCn'nnn_
Cuts at: 0 352 1122
Size: 352 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 183 1122
Size: 40 143 939
Fragments arranged by size:
939 143 40
EcoICRI GAG'CTC
Cuts at: 0 489 1122
Size: 489 633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 343 391 580 787 793 979 1122
Size: 32 311 48 189 207 6 186 143
Fragments arranged by size:
311 207 189 186 143 48 32 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 991 1122
Size: 991 131
EcoO109I rG'GnC_Cy
Cuts at: 0 98 535 1122
Size: 98 437 587
Fragments arranged by size:
587 437 98
FspI TGC'GCA
Cuts at: 0 1063 1122
Size: 1063 59
HaeII r_GCGC'y
Cuts at: 0 128 432 640 652 1122
Size: 128 304 208 12 470
Fragments arranged by size:
470 304 208 128 12
KasI G'GCGC_C
Cuts at: 0 636 1122
Size: 636 486
MspA1I CmG'CkG
Cuts at: 0 112 332 479 708 790 1122
Size: 112 220 147 229 82 332
Fragments arranged by size:
332 229 220 147 112 82
NarI GG'CG_CC
Cuts at: 0 637 1122
Size: 637 485
NspI r_CATG'y
Cuts at: 0 177 1122
Size: 177 945
PasI CC'CwG_GG
Cuts at: 0 748 1122
Size: 748 374
PfoI T'CCnGG_A
Cuts at: 0 368 1122
Size: 368 754
PpuMI rG'GwC_Cy
Cuts at: 0 535 1122
Size: 535 587
PshAI GACnn'nnGTC
Cuts at: 0 805 1122
Size: 805 317
PspXI vC'TCGA_Gb
Cuts at: 0 550 916 1122
Size: 550 366 206
Fragments arranged by size:
550 366 206
PstI C_TGCA'G
Cuts at: 0 97 124 467 815 1122
Size: 97 27 343 348 307
Fragments arranged by size:
348 343 307 97 27
PvuII CAG'CTG
Cuts at: 0 479 1122
Size: 479 643
SacI G_AGCT'C
Cuts at: 0 491 1122
Size: 491 631
SacII CC_GC'GG
Cuts at: 0 333 1122
Size: 333 789
SanDI GG'GwC_CC
Cuts at: 0 535 1122
Size: 535 587
SapI GCTCTTCn'nnn_
Cuts at: 0 352 1122
Size: 352 770
SfcI C'TryA_G
Cuts at: 0 93 120 463 811 1122
Size: 93 27 343 348 311
Fragments arranged by size:
348 343 311 93 27
SfoI GGC'GCC
Cuts at: 0 638 1122
Size: 638 484
SmaI CCC'GGG
Cuts at: 0 302 1122
Size: 302 820
SmlI C'TyrA_G
Cuts at: 0 550 916 1075 1122
Size: 550 366 159 47
Fragments arranged by size:
550 366 159 47
SphI G_CATG'C
Cuts at: 0 177 1122
Size: 177 945
StuI AGG'CCT
Cuts at: 0 314 565 1107 1122
Size: 314 251 542 15
Fragments arranged by size:
542 314 251 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 659 1122
Size: 659 463
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 64 1122
Size: 32 32 1058
Fragments arranged by size:
1058 32 32
XhoI C'TCGA_G
Cuts at: 0 550 916 1122
Size: 550 366 206
Fragments arranged by size:
550 366 206
XmaI C'CCGG_G
Cuts at: 0 300 1122
Size: 300 822
ZraI GAC'GTC
Cuts at: 0 366 1122
Size: 366 756
Enzymes that do cut:
AatII AlfI AlwNI ApaLI ApoI AvaI BaeI BanI
BanII BbeI BcgI BglI Bme1580I BmrI BpmI BpuEI
BsaI BsaHI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BssHII BssSI BtgI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
KasI MspA1I NarI NspI PasI PfoI PpuMI PshAI
PspXI PstI PvuII SacI SacII SanDI SapI SfcI
SfoI SmaI SmlI SphI StuI TaqII TstI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI AscI AseI AsiSI
AvrII BamHI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI
BsaXI BseYI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV
FalI FseI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PsiI PspOMI PsrI PvuI RsrII
SalI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI StyI SwaI TatI Tth111I XbaI
XcmI XmnI