Restriction map for
pFN21AE3942
(Linear) (Six-base) MAPSORT of: KIEE3942.seq Check: 9576 from: 1 to: 1299
KIEE3942.orf
With 182 enzymes: SgfI *
March 22, 2012 16:55 ..
AccI GT'mk_AC
Cuts at: 0 128 1238 1292 1299
Size: 128 1110 54 7
Fragments arranged by size:
1110 128 54 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1132 1299
Size: 1007 125 167
Fragments arranged by size:
1007 167 125
AflIII A'CryG_T
Cuts at: 0 226 1299
Size: 226 1073
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 472 504 1299
Size: 472 32 795
Fragments arranged by size:
795 472 32
BanI G'GyrC_C
Cuts at: 0 302 860 915 1299
Size: 302 558 55 384
Fragments arranged by size:
558 384 302 55
BanII G_rGCy'C
Cuts at: 0 850 927 1299
Size: 850 77 372
Fragments arranged by size:
850 372 77
BbeI G_GCGC'C
Cuts at: 0 864 1299
Size: 864 435
BbsI GAAGACnn'nnnn_
Cuts at: 0 151 799 1299
Size: 151 648 500
Fragments arranged by size:
648 500 151
BbvCI CC'TCA_GC
Cuts at: 0 642 1299
Size: 642 657
BclI T'GATC_A
Cuts at: 0 440 1299
Size: 440 859
BglI GCCn_nnn'nGGC
Cuts at: 0 470 1185 1268 1299
Size: 470 715 83 31
Fragments arranged by size:
715 470 83 31
Bme1580I G_kGCm'C
Cuts at: 0 305 324 918 1299
Size: 305 19 594 381
Fragments arranged by size:
594 381 305 19
BmgBI CAC'GTC
Cuts at: 0 48 1299
Size: 48 1251
BmrI ACTGGGnnnn_n'
Cuts at: 0 853 1299
Size: 853 446
BmtI G_CTAG'C
Cuts at: 0 703 1299
Size: 703 596
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1081 1299
Size: 1081 218
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 649 891 941 1067 1299
Size: 354 295 242 50 126 232
Fragments arranged by size:
354 295 242 232 126 50
Bpu10I CC'TnA_GC
Cuts at: 0 642 1299
Size: 642 657
BsaI GGTCTCn'nnnn_
Cuts at: 0 372 1299
Size: 372 927
BsaHI Gr'CG_yC
Cuts at: 0 353 861 1299
Size: 353 508 438
Fragments arranged by size:
508 438 353
BsaWI w'CCGG_w
Cuts at: 0 218 738 1299
Size: 218 520 561
Fragments arranged by size:
561 520 218
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 864 894 1299
Size: 25 839 30 405
Fragments arranged by size:
839 405 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1030 1033 1087 1090 1138 1299
Size: 1030 3 54 3 48 161
Fragments arranged by size:
1030 161 54 48 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 328 1194 1299
Size: 148 180 866 105
Fragments arranged by size:
866 180 148 105
BsiHKAI G_wGCw'C
Cuts at: 0 927 1299
Size: 927 372
Bsp1286I G_dGCh'C
Cuts at: 0 305 324 850 918 927 1299
Size: 305 19 526 68 9 372
Fragments arranged by size:
526 372 305 68 19 9
BspEI T'CCGG_A
Cuts at: 0 738 1299
Size: 738 561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 143 1299
Size: 143 1156
BsrBI CCG'CTC
Cuts at: 0 1128 1257 1299
Size: 1128 129 42
Fragments arranged by size:
1128 129 42
BsrDI GCAATG_nn'
Cuts at: 0 190 413 1299
Size: 190 223 886
Fragments arranged by size:
886 223 190
BsrFI r'CCGG_y
Cuts at: 0 1184 1299
Size: 1184 115
BstEII G'GTnAC_C
Cuts at: 0 934 1299
Size: 934 365
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 1299
Size: 426 873
Bsu36I CC'TnA_GG
Cuts at: 0 751 1299
Size: 751 548
BtgI C'CryG_G
Cuts at: 0 174 212 590 1299
Size: 174 38 378 709
Fragments arranged by size:
709 378 174 38
BtsI GCAGTG_nn'
Cuts at: 0 14 322 521 585 722 1299
Size: 14 308 199 64 137 577
Fragments arranged by size:
577 308 199 137 64 14
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1231 1266 1299
Size: 1231 35 33
Fragments arranged by size:
1231 35 33
EaeI y'GGCC_r
Cuts at: 0 375 587 593 659 790 898 1299
Size: 375 212 6 66 131 108 401
Fragments arranged by size:
401 375 212 131 108 66 6
EarI CTCTTCn'nnn_
Cuts at: 0 986 1007 1299
Size: 986 21 292
Fragments arranged by size:
986 292 21
EcoICRI GAG'CTC
Cuts at: 0 925 1299
Size: 925 374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 649 891 941 1007 1067 1132 1299
Size: 354 295 242 50 66 60 65 167
Fragments arranged by size:
354 295 242 167 66 65 60 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1201 1299
Size: 1201 98
EcoO109I rG'GnC_Cy
Cuts at: 0 962 1299
Size: 962 337
HaeII r_GCGC'y
Cuts at: 0 864 1299
Size: 864 435
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 510 542 803 835 864 896 1299
Size: 510 32 261 32 29 32 403
Fragments arranged by size:
510 403 261 32 32 32 29
HincII GTy'rAC
Cuts at: 0 456 1299
Size: 456 843
KasI G'GCGC_C
Cuts at: 0 860 1299
Size: 860 439
MfeI C'AATT_G
Cuts at: 0 1248 1299
Size: 1248 51
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 1299
Size: 44 1255
MscI TGG'CCA
Cuts at: 0 377 589 595 661 900 1299
Size: 377 212 6 66 239 399
Fragments arranged by size:
399 377 239 212 66 6
MslI CAynn'nnrTG
Cuts at: 0 418 1036 1299
Size: 418 618 263
Fragments arranged by size:
618 418 263
MspA1I CmG'CkG
Cuts at: 0 176 646 888 1299
Size: 176 470 242 411
Fragments arranged by size:
470 411 242 176
NaeI GCC'GGC
Cuts at: 0 1186 1299
Size: 1186 113
NarI GG'CG_CC
Cuts at: 0 861 1299
Size: 861 438
NcoI C'CATG_G
Cuts at: 0 590 1299
Size: 590 709
NgoMIV G'CCGG_C
Cuts at: 0 1184 1299
Size: 1184 115
NheI G'CTAG_C
Cuts at: 0 699 1299
Size: 699 600
NspI r_CATG'y
Cuts at: 0 230 1299
Size: 230 1069
PasI CC'CwG_GG
Cuts at: 0 930 1299
Size: 930 369
PciI A'CATG_T
Cuts at: 0 226 1299
Size: 226 1073
PpuMI rG'GwC_Cy
Cuts at: 0 962 1299
Size: 962 337
PshAI GACnn'nnGTC
Cuts at: 0 1045 1299
Size: 1045 254
PstI C_TGCA'G
Cuts at: 0 887 1280 1299
Size: 887 393 19
Fragments arranged by size:
887 393 19
PvuII CAG'CTG
Cuts at: 0 646 888 1299
Size: 646 242 411
Fragments arranged by size:
646 411 242
SacI G_AGCT'C
Cuts at: 0 927 1299
Size: 927 372
SacII CC_GC'GG
Cuts at: 0 177 1299
Size: 177 1122
SexAI A'CCwGG_T
Cuts at: 0 14 1299
Size: 14 1285
SfcI C'TryA_G
Cuts at: 0 883 1276 1299
Size: 883 393 23
Fragments arranged by size:
883 393 23
SfoI GGC'GCC
Cuts at: 0 862 1299
Size: 862 437
SpeI A'CTAG_T
Cuts at: 0 154 1299
Size: 154 1145
StuI AGG'CCT
Cuts at: 0 244 397 856 1207 1299
Size: 244 153 459 351 92
Fragments arranged by size:
459 351 244 153 92
StyI C'CwwG_G
Cuts at: 0 56 590 1099 1299
Size: 56 534 509 200
Fragments arranged by size:
534 509 200 56
TatI w'GTAC_w
Cuts at: 0 250 1299
Size: 250 1049
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1122 1154 1299
Size: 1122 32 145
Fragments arranged by size:
1122 145 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 426 582 1299
Size: 426 156 717
Fragments arranged by size:
717 426 156
Enzymes that do cut:
AccI AcuI AflIII AloI BanI BanII BbeI BbsI
BbvCI BclI BglI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstEII
BstXI Bsu36I BtgI BtsI CspCI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PciI PpuMI PshAI PstI PvuII
SacI SacII SexAI SfcI SfoI SpeI StuI StyI
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BcgI BciVI BfrBI
BglII BlpI BpuEI BsaAI BsaBI BseYI BsiEI BsiWI
BsmI BsmBI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstYI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MluI NdeI NotI NruI NsiI PacI
PflMI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI
SspI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI
ZraI