Restriction map for
pFN21AE3941
(Linear) (Six-base) MAPSORT of: KIEE3941.seq Check: 7969 from: 1 to: 1761
KIEE3941.orf
With 182 enzymes: SgfI *
March 22, 2012 16:55 ..
AccI GT'mk_AC
Cuts at: 0 1061 1761
Size: 1061 700
AfeI AGC'GCT
Cuts at: 0 1348 1761
Size: 1348 413
AflIII A'CryG_T
Cuts at: 0 1316 1761
Size: 1316 445
AleI CACnn'nnGTG
Cuts at: 0 832 1097 1516 1761
Size: 832 265 419 245
Fragments arranged by size:
832 419 265 245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 460 1761
Size: 460 1301
AlwNI CAG_nnn'CTG
Cuts at: 0 12 72 243 1054 1761
Size: 12 60 171 811 707
Fragments arranged by size:
811 707 171 60 12
ApaI G_GGCC'C
Cuts at: 0 590 1761
Size: 590 1171
ApaLI G'TGCA_C
Cuts at: 0 1320 1761
Size: 1320 441
AvaI C'yCGr_G
Cuts at: 0 512 590 1024 1441 1633 1761
Size: 512 78 434 417 192 128
Fragments arranged by size:
512 434 417 192 128 78
BanI G'GyrC_C
Cuts at: 0 346 492 675 748 1741 1761
Size: 346 146 183 73 993 20
Fragments arranged by size:
993 346 183 146 73 20
BanII G_rGCy'C
Cuts at: 0 373 425 433 590 1414 1663 1761
Size: 373 52 8 157 824 249 98
Fragments arranged by size:
824 373 249 157 98 52 8
BbeI G_GCGC'C
Cuts at: 0 752 1761
Size: 752 1009
BciVI GTATCCnnnnn_n'
Cuts at: 0 979 1761
Size: 979 782
BglI GCCn_nnn'nGGC
Cuts at: 0 77 124 133 466 616 1761
Size: 77 47 9 333 150 1145
Fragments arranged by size:
1145 333 150 77 47 9
Bme1580I G_kGCm'C
Cuts at: 0 497 590 637 680 1324 1433 1744 1761
Size: 497 93 47 43 644 109 311 17
Fragments arranged by size:
644 497 311 109 93 47 43 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 939 1761
Size: 939 822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 934 1057 1761
Size: 598 336 123 704
Fragments arranged by size:
704 598 336 123
Bpu10I CC'TnA_GC
Cuts at: 0 899 1761
Size: 899 862
BsaBI GATnn'nnATC
Cuts at: 0 859 1761
Size: 859 902
BsaHI Gr'CG_yC
Cuts at: 0 410 749 1505 1535 1580 1711 1761
Size: 410 339 756 30 45 131 50
Fragments arranged by size:
756 410 339 131 50 45 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 829 859 1761
Size: 400 30 399 30 902
Fragments arranged by size:
902 400 399 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 811 820 853 1603 1761
Size: 811 9 33 750 158
Fragments arranged by size:
811 750 158 33 9
BseYI C'CCAG_C
Cuts at: 0 48 75 679 1210 1276 1696 1761
Size: 48 27 604 531 66 420 65
Fragments arranged by size:
604 531 420 66 65 48 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1121 1761
Size: 1121 640
BsiEI CG_ry'CG
Cuts at: 0 311 455 548 1721 1761
Size: 311 144 93 1173 40
Fragments arranged by size:
1173 311 144 93 40
BsiHKAI G_wGCw'C
Cuts at: 0 373 1324 1761
Size: 373 951 437
Fragments arranged by size:
951 437 373
BsmBI CGTCTCn'nnnn_
Cuts at: 0 574 1761
Size: 574 1187
Bsp1286I G_dGCh'C
Cuts at: 0 373 425 433 497 590 637 680 1324
Size: 373 52 8 64 93 47 43 644
Cuts at: 1324 1414 1433 1663 1744 1761
Size: 90 19 230 81 17
Fragments arranged by size:
644 373 230 93 90 81 64 52
47 43 19 17 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 969 1761
Size: 969 792
BsrBI CCG'CTC
Cuts at: 0 735 923 1193 1494 1761
Size: 735 188 270 301 267
Fragments arranged by size:
735 301 270 267 188
BsrFI r'CCGG_y
Cuts at: 0 310 412 450 543 547 1582 1713 1761
Size: 310 102 38 93 4 1035 131 48
Fragments arranged by size:
1035 310 131 102 93 48 38 4
BsrGI T'GTAC_A
Cuts at: 0 302 857 1761
Size: 302 555 904
Fragments arranged by size:
904 555 302
BssSI C'ACGA_G
Cuts at: 0 292 949 1761
Size: 292 657 812
Fragments arranged by size:
812 657 292
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 1761
Size: 72 1689
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1518 1761
Size: 1518 243
BstYI r'GATC_y
Cuts at: 0 23 239 323 1761
Size: 23 216 84 1438
Fragments arranged by size:
1438 216 84 23
BtgI C'CryG_G
Cuts at: 0 464 530 611 830 1310 1457 1721 1761
Size: 464 66 81 219 480 147 264 40
Fragments arranged by size:
480 464 264 219 147 81 66 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 104 1761
Size: 104 1657
BtsI GCAGTG_nn'
Cuts at: 0 378 890 1761
Size: 378 512 871
Fragments arranged by size:
871 512 378
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1131 1166 1761
Size: 1131 35 595
Fragments arranged by size:
1131 595 35
DraIII CAC_nnn'GTG
Cuts at: 0 336 1761
Size: 336 1425
DrdI GACnn_nn'nnGTC
Cuts at: 0 1234 1761
Size: 1234 527
EaeI y'GGCC_r
Cuts at: 0 143 278 308 452 545 608 686 849
Size: 143 135 30 144 93 63 78 163
Cuts at: 849 1074 1139 1166 1625 1653 1718 1761
Size: 225 65 27 459 28 65 43
Fragments arranged by size:
459 225 163 144 143 135 93 78
65 65 63 43 30 28 27
EagI C'GGCC_G
Cuts at: 0 308 452 545 1718 1761
Size: 308 144 93 1173 43
Fragments arranged by size:
1173 308 144 93 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 928 1404 1761
Size: 928 476 357
Fragments arranged by size:
928 476 357
EcoICRI GAG'CTC
Cuts at: 0 371 1761
Size: 371 1390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 934 1057 1761
Size: 598 336 123 704
Fragments arranged by size:
704 598 336 123
EcoO109I rG'GnC_Cy
Cuts at: 0 586 587 1020 1236 1703 1754 1761
Size: 586 1 433 216 467 51 7
Fragments arranged by size:
586 467 433 216 51 7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 213 1761
Size: 213 1548
FseI GG_CCGG'CC
Cuts at: 0 551 1761
Size: 551 1210
HaeII r_GCGC'y
Cuts at: 0 72 197 573 752 1350 1761
Size: 72 125 376 179 598 411
Fragments arranged by size:
598 411 376 179 125 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 1761
Size: 400 32 1329
Fragments arranged by size:
1329 400 32
HincII GTy'rAC
Cuts at: 0 1548 1761
Size: 1548 213
KasI G'GCGC_C
Cuts at: 0 748 1761
Size: 748 1013
MscI TGG'CCA
Cuts at: 0 688 1141 1168 1655 1761
Size: 688 453 27 487 106
Fragments arranged by size:
688 487 453 106 27
MslI CAynn'nnrTG
Cuts at: 0 832 1097 1405 1516 1761
Size: 832 265 308 111 245
Fragments arranged by size:
832 308 265 245 111
MspA1I CmG'CkG
Cuts at: 0 179 397 444 466 504 576 613 912
Size: 179 218 47 22 38 72 37 299
Cuts at: 912 992 1080 1175 1224 1312 1459 1641 1723
Size: 80 88 95 49 88 147 182 82
Cuts at: 1723 1761
Size: 38
Fragments arranged by size:
299 218 182 179 147 95 88 88
82 80 72 49 47 38 38 37
22
NaeI GCC'GGC
Cuts at: 0 312 414 452 545 549 1584 1715 1761
Size: 312 102 38 93 4 1035 131 46
Fragments arranged by size:
1035 312 131 102 93 46 38 4
NarI GG'CG_CC
Cuts at: 0 749 1761
Size: 749 1012
NcoI C'CATG_G
Cuts at: 0 530 830 1761
Size: 530 300 931
Fragments arranged by size:
931 530 300
NgoMIV G'CCGG_C
Cuts at: 0 310 412 450 543 547 1582 1713 1761
Size: 310 102 38 93 4 1035 131 48
Fragments arranged by size:
1035 310 131 102 93 48 38 4
NotI GC'GGCC_GC
Cuts at: 0 1718 1761
Size: 1718 43
NspI r_CATG'y
Cuts at: 0 1320 1761
Size: 1320 441
PasI CC'CwG_GG
Cuts at: 0 726 1761
Size: 726 1035
PciI A'CATG_T
Cuts at: 0 1316 1761
Size: 1316 445
PflMI CCAn_nnn'nTGG
Cuts at: 0 1623 1761
Size: 1623 138
PfoI T'CCnGG_A
Cuts at: 0 1616 1761
Size: 1616 145
PpuMI rG'GwC_Cy
Cuts at: 0 1020 1236 1754 1761
Size: 1020 216 518 7
Fragments arranged by size:
1020 518 216 7
PspOMI G'GGCC_C
Cuts at: 0 586 1761
Size: 586 1175
PstI C_TGCA'G
Cuts at: 0 323 449 1761
Size: 323 126 1312
Fragments arranged by size:
1312 323 126
PvuII CAG'CTG
Cuts at: 0 444 1080 1175 1761
Size: 444 636 95 586
Fragments arranged by size:
636 586 444 95
SacI G_AGCT'C
Cuts at: 0 373 1761
Size: 373 1388
SacII CC_GC'GG
Cuts at: 0 467 614 1313 1460 1724 1761
Size: 467 147 699 147 264 37
Fragments arranged by size:
699 467 264 147 147 37
SanDI GG'GwC_CC
Cuts at: 0 1020 1761
Size: 1020 741
SfcI C'TryA_G
Cuts at: 0 319 445 1761
Size: 319 126 1316
Fragments arranged by size:
1316 319 126
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 124 1761
Size: 124 1637
SfoI GGC'GCC
Cuts at: 0 750 1761
Size: 750 1011
SgrAI Cr'CCGG_yG
Cuts at: 0 1582 1713 1761
Size: 1582 131 48
Fragments arranged by size:
1582 131 48
SmaI CCC'GGG
Cuts at: 0 592 1026 1761
Size: 592 434 735
Fragments arranged by size:
735 592 434
StuI AGG'CCT
Cuts at: 0 118 557 820 868 1761
Size: 118 439 263 48 893
Fragments arranged by size:
893 439 263 118 48
StyI C'CwwG_G
Cuts at: 0 530 830 1761
Size: 530 300 931
Fragments arranged by size:
931 530 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1599 1761
Size: 1599 162
TatI w'GTAC_w
Cuts at: 0 302 857 1256 1761
Size: 302 555 399 505
Fragments arranged by size:
555 505 399 302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 573 1761
Size: 573 1188
XmaI C'CCGG_G
Cuts at: 0 590 1024 1761
Size: 590 434 737
Fragments arranged by size:
737 590 434
Enzymes that do cut:
AccI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BciVI BglI Bme1580I BplI
BpmI Bpu10I BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoO109I
FalI FseI HaeII Hin4I HincII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SfcI SfiI SfoI SgrAI SmaI StuI
StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI
BmrI BmtI BpuEI BsaI BsaAI BsaWI BsiWI BsmI
BspEI BspHI BsrDI BssHII BstBI BstEII BstZ17I Bsu36I
ClaI DraI EarI EcoNI EcoRI EcoRV FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI
SexAI SgfI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmnI ZraI