(Linear) (Six-base) MAPSORT of: KIEE3939.seq  Check: 398  from: 1  to: 1554



KIEE3939.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:54  ..

AccI GT'mk_AC

Cuts at:      0    1434    1554 
   Size:       1434     120
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1078    1554 
   Size:       1078     476
 
AflII C'TTAA_G

Cuts at:      0      75    1554 
   Size:         75    1479
 
AflIII A'CryG_T

Cuts at:      0      26    1554 
   Size:         26    1528
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     272    1382    1554 
   Size:        272    1110     172

  Fragments arranged by size: 

                1110     272     172
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     970    1002    1554 
   Size:        970      32     552

  Fragments arranged by size: 

                 970     552      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     712    1554 
   Size:        712     842
 
ApoI r'AATT_y

Cuts at:      0    1135    1406    1554 
   Size:       1135     271     148

  Fragments arranged by size: 

                1135     271     148
 
AseI AT'TA_AT

Cuts at:      0     188    1554 
   Size:        188    1366
 
BamHI G'GATC_C

Cuts at:      0     418    1554 
   Size:        418    1136
 
BanII G_rGCy'C

Cuts at:      0    1026    1554 
   Size:       1026     528
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     229    1300    1508    1554 
   Size:        229    1071     208      46

  Fragments arranged by size: 

                1071     229     208      46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     836    1554 
   Size:        836     718
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     681    1554 
   Size:        681     873
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     603     644    1554 
   Size:        603      41     910

  Fragments arranged by size: 

                 910     603      41
 
BsaAI yAC'GTr

Cuts at:      0      29    1554 
   Size:         29    1525
 
BsaBI GATnn'nnATC

Cuts at:      0     713    1554 
   Size:        713     841
 
BsaHI Gr'CG_yC

Cuts at:      0     920    1554 
   Size:        920     634
 
BsaWI w'CCGG_w

Cuts at:      0     899    1554 
   Size:        899     655
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     395     425    1554 
   Size:        395      30    1129

  Fragments arranged by size: 

                1129     395      30
 
BseYI C'CCAG_C

Cuts at:      0     301    1554 
   Size:        301    1253
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746    1554 
   Size:        746     808
 
BsiHKAI G_wGCw'C

Cuts at:      0      34    1554 
   Size:         34    1520
 
BsmI GAATG_Cn'

Cuts at:      0     523    1554 
   Size:        523    1031
 
Bsp1286I G_dGCh'C

Cuts at:      0      34    1026    1554 
   Size:         34     992     528

  Fragments arranged by size: 

                 992     528      34
 
BspEI T'CCGG_A

Cuts at:      0     899    1554 
   Size:        899     655
 
BspHI T'CATG_A

Cuts at:      0     141    1554 
   Size:        141    1413
 
BsrDI GCAATG_nn'

Cuts at:      0      69     650    1554 
   Size:         69     581     904

  Fragments arranged by size: 

                 904     581      69
 
BssSI C'ACGA_G

Cuts at:      0    1331    1554 
   Size:       1331     223
 
BstYI r'GATC_y

Cuts at:      0     309     418     708    1554 
   Size:        309     109     290     846

  Fragments arranged by size: 

                 846     309     290     109
 
BtgI C'CryG_G

Cuts at:      0    1117    1554 
   Size:       1117     437
 
BtsI GCAGTG_nn'

Cuts at:      0     866    1554 
   Size:        866     688
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     662     697    1554 
   Size:        662      35     857

  Fragments arranged by size: 

                 857     662      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     681    1078    1554 
   Size:        681     397     476

  Fragments arranged by size: 

                 681     476     397
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48    1554 
   Size:         16      32    1506

  Fragments arranged by size: 

                1506      32      16
 
HindIII A'AGCT_T

Cuts at:      0     579    1554 
   Size:        579     975
 
MfeI C'AATT_G

Cuts at:      0     615    1554 
   Size:        615     939
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      21    1554 
   Size:         21    1533
 
MslI CAynn'nnrTG

Cuts at:      0     217    1130    1554 
   Size:        217     913     424

  Fragments arranged by size: 

                 913     424     217
 
MspA1I CmG'CkG

Cuts at:      0     760    1309    1461    1554 
   Size:        760     549     152      93

  Fragments arranged by size: 

                 760     549     152      93
 
NcoI C'CATG_G

Cuts at:      0    1117    1554 
   Size:       1117     437
 
PacI TTA_AT'TAA

Cuts at:      0     188    1554 
   Size:        188    1366
 
PmlI CAC'GTG

Cuts at:      0      29    1554 
   Size:         29    1525
 
PvuII CAG'CTG

Cuts at:      0     760    1309    1461    1554 
   Size:        760     549     152      93

  Fragments arranged by size: 

                 760     549     152      93
 
SfcI C'TryA_G

Cuts at:      0      35     164     909    1354    1554 
   Size:         35     129     745     445     200

  Fragments arranged by size: 

                 745     445     200     129      35
 
SmlI C'TyrA_G

Cuts at:      0      75     582     659    1554 
   Size:         75     507      77     895

  Fragments arranged by size: 

                 895     507      77      75
 
SspI AAT'ATT

Cuts at:      0     893    1554 
   Size:        893     661
 
StyI C'CwwG_G

Cuts at:      0     626    1117    1554 
   Size:        626     491     437

  Fragments arranged by size: 

                 626     491     437
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1400    1554 
   Size:       1400     154
 
Tth111I GACn'n_nGTC

Cuts at:      0    1246    1554 
   Size:       1246     308
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     430     529    1554 
   Size:        430      99    1025

  Fragments arranged by size: 

                1025     430      99
 
XmnI GAAnn'nnTTC

Cuts at:      0    1505    1554 
   Size:       1505      49
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AlfI     AloI    AlwNI     ApoI
     AseI    BamHI    BanII     BbsI     BmrI     BpmI    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BsrDI    BssSI    BstYI     BtgI     BtsI
    CspCI  Eco57MI    Hin4I  HindIII     MfeI     MmeI     MslI   MspA1I
     NcoI     PacI     PmlI    PvuII     SfcI     SmlI     SspI     StyI
    TaqII  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BseRI
    BsiEI    BsiWI    BsmBI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     TatI     TstI     XbaI     XhoI     XmaI     ZraI