(Linear) (Six-base) MAPSORT of: KIEE3937.seq  Check: 3533  from: 1  to: 597



KIEE3937.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:54  ..

AccI GT'mk_AC

Cuts at:      0     248     597 
   Size:        248     349
 
AflII C'TTAA_G

Cuts at:      0     292     597 
   Size:        292     305
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     424     458     597 
   Size:        424      34     139

  Fragments arranged by size: 

                 424     139      34
 
Bme1580I G_kGCm'C

Cuts at:      0     218     597 
   Size:        218     379
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     357     473     597 
   Size:        357     116     124

  Fragments arranged by size: 

                 357     124     116
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     538     597 
   Size:        432     106      59

  Fragments arranged by size: 

                 432     106      59
 
BsiHKAI G_wGCw'C

Cuts at:      0     442     597 
   Size:        442     155
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     442     597 
   Size:        218     224     155

  Fragments arranged by size: 

                 224     218     155
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     369     597 
   Size:        369     228
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     417     597 
   Size:        417     180
 
BtgI C'CryG_G

Cuts at:      0     164     597 
   Size:        164     433
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     237     597 
   Size:        237     360
 
BtsI GCAGTG_nn'

Cuts at:      0     179     597 
   Size:        179     418
 
ClaI AT'CG_AT

Cuts at:      0     146     597 
   Size:        146     451
 
EaeI y'GGCC_r

Cuts at:      0     407     597 
   Size:        407     190
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     357     473     597 
   Size:        357     116     124

  Fragments arranged by size: 

                 357     124     116
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     327     597 
   Size:        327     270
 
MscI TGG'CCA

Cuts at:      0     409     597 
   Size:        409     188
 
NcoI C'CATG_G

Cuts at:      0     164     597 
   Size:        164     433
 
NspI r_CATG'y

Cuts at:      0     222     556     597 
   Size:        222     334      41

  Fragments arranged by size: 

                 334     222      41
 
SmlI C'TyrA_G

Cuts at:      0     292     597 
   Size:        292     305
 
SspI AAT'ATT

Cuts at:      0      32     597 
   Size:         32     565
 
StyI C'CwwG_G

Cuts at:      0     164     597 
   Size:        164     433
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     278     597 
   Size:        278     319
 

 Enzymes that do cut:

     AccI    AflII     BcgI Bme1580I     BpmI     BsgI  BsiHKAI Bsp1286I
    BspMI    BstXI     BtgI    BtgZI     BtsI     ClaI     EaeI  Eco57MI
     MmeI     MscI     NcoI     NspI     SmlI     SspI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI