Restriction map for
pFN21AE3934
(Linear) (Six-base) MAPSORT of: KIEE3934.seq Check: 8998 from: 1 to: 1635
KIEE3934.orf
With 182 enzymes: SgfI *
March 22, 2012 16:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 1057 1391 1635
Size: 843 214 334 244
Fragments arranged by size:
843 334 244 214
AfeI AGC'GCT
Cuts at: 0 586 958 1635
Size: 586 372 677
Fragments arranged by size:
677 586 372
AleI CACnn'nnGTG
Cuts at: 0 109 1635
Size: 109 1526
AlwNI CAG_nnn'CTG
Cuts at: 0 378 1243 1635
Size: 378 865 392
Fragments arranged by size:
865 392 378
ApaI G_GGCC'C
Cuts at: 0 369 1635
Size: 369 1266
ApaLI G'TGCA_C
Cuts at: 0 878 1635
Size: 878 757
ApoI r'AATT_y
Cuts at: 0 196 418 847 1635
Size: 196 222 429 788
Fragments arranged by size:
788 429 222 196
AvaI C'yCGr_G
Cuts at: 0 40 834 936 1568 1635
Size: 40 794 102 632 67
Fragments arranged by size:
794 632 102 67 40
BamHI G'GATC_C
Cuts at: 0 777 1635
Size: 777 858
BanI G'GyrC_C
Cuts at: 0 682 723 901 1635
Size: 682 41 178 734
Fragments arranged by size:
734 682 178 41
BanII G_rGCy'C
Cuts at: 0 369 698 1166 1369 1635
Size: 369 329 468 203 266
Fragments arranged by size:
468 369 329 266 203
BbsI GAAGACnn'nnnn_
Cuts at: 0 1135 1532 1553 1635
Size: 1135 397 21 82
Fragments arranged by size:
1135 397 82 21
BglI GCCn_nnn'nGGC
Cuts at: 0 496 1635
Size: 496 1139
Bme1580I G_kGCm'C
Cuts at: 0 13 369 728 882 904 1504 1607 1635
Size: 13 356 359 154 22 600 103 28
Fragments arranged by size:
600 359 356 154 103 28 22 13
BmgBI CAC'GTC
Cuts at: 0 798 843 1635
Size: 798 45 792
Fragments arranged by size:
798 792 45
BmtI G_CTAG'C
Cuts at: 0 1316 1635
Size: 1316 319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1398 1635
Size: 1398 237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 421 736 1635
Size: 214 207 315 899
Fragments arranged by size:
899 315 214 207
Bpu10I CC'TnA_GC
Cuts at: 0 1183 1258 1279 1635
Size: 1183 75 21 356
Fragments arranged by size:
1183 356 75 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 1635
Size: 319 1316
BsaI GGTCTCn'nnnn_
Cuts at: 0 162 590 1635
Size: 162 428 1045
Fragments arranged by size:
1045 428 162
BsaAI yAC'GTr
Cuts at: 0 1602 1635
Size: 1602 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 212 242 1635
Size: 212 30 1393
Fragments arranged by size:
1393 212 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1450 1635
Size: 1450 185
BseYI C'CCAG_C
Cuts at: 0 368 1635
Size: 368 1267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1234 1635
Size: 796 438 401
Fragments arranged by size:
796 438 401
BsiHKAI G_wGCw'C
Cuts at: 0 41 882 1369 1635
Size: 41 841 487 266
Fragments arranged by size:
841 487 266 41
Bsp1286I G_dGCh'C
Cuts at: 0 13 41 369 698 728 882 904 1166
Size: 13 28 328 329 30 154 22 262
Cuts at: 1166 1369 1504 1607 1635
Size: 203 135 103 28
Fragments arranged by size:
329 328 262 203 154 135 103 30
28 28 22 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 180 1635
Size: 180 1455
BsrBI CCG'CTC
Cuts at: 0 426 1635
Size: 426 1209
BsrFI r'CCGG_y
Cuts at: 0 1283 1635
Size: 1283 352
BstEII G'GTnAC_C
Cuts at: 0 990 1635
Size: 990 645
BstXI CCAn_nnnn'nTGG
Cuts at: 0 678 744 1635
Size: 678 66 891
Fragments arranged by size:
891 678 66
BstYI r'GATC_y
Cuts at: 0 777 1635
Size: 777 858
BtsI GCAGTG_nn'
Cuts at: 0 61 1635
Size: 61 1574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 755 790 1635
Size: 755 35 845
Fragments arranged by size:
845 755 35
DraI TTT'AAA
Cuts at: 0 393 1635
Size: 393 1242
DraIII CAC_nnn'GTG
Cuts at: 0 56 1467 1635
Size: 56 1411 168
Fragments arranged by size:
1411 168 56
DrdI GACnn_nn'nnGTC
Cuts at: 0 796 1635
Size: 796 839
EaeI y'GGCC_r
Cuts at: 0 545 1469 1514 1635
Size: 545 924 45 121
Fragments arranged by size:
924 545 121 45
EarI CTCTTCn'nnn_
Cuts at: 0 1367 1382 1635
Size: 1367 15 253
Fragments arranged by size:
1367 253 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1593 1635
Size: 1593 42
EcoICRI GAG'CTC
Cuts at: 0 1367 1635
Size: 1367 268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 421 736 843 1057 1391 1635
Size: 214 207 315 107 214 334 244
Fragments arranged by size:
334 315 244 214 214 207 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 703 1635
Size: 703 932
EcoO109I rG'GnC_Cy
Cuts at: 0 1146 1635
Size: 1146 489
EcoRI G'AATT_C
Cuts at: 0 847 1635
Size: 847 788
EcoRV GAT'ATC
Cuts at: 0 483 1635
Size: 483 1152
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 547 1635
Size: 363 184 1088
Fragments arranged by size:
1088 363 184
HaeII r_GCGC'y
Cuts at: 0 48 588 960 1319 1635
Size: 48 540 372 359 316
Fragments arranged by size:
540 372 359 316 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 1635
Size: 175 32 1428
Fragments arranged by size:
1428 175 32
HincII GTy'rAC
Cuts at: 0 1086 1635
Size: 1086 549
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 683 911 1031 1635
Size: 53 630 228 120 604
Fragments arranged by size:
630 604 228 120 53
MscI TGG'CCA
Cuts at: 0 1471 1635
Size: 1471 164
MslI CAynn'nnrTG
Cuts at: 0 109 757 1635
Size: 109 648 878
Fragments arranged by size:
878 648 109
MspA1I CmG'CkG
Cuts at: 0 258 378 399 1635
Size: 258 120 21 1236
Fragments arranged by size:
1236 258 120 21
NaeI GCC'GGC
Cuts at: 0 1285 1635
Size: 1285 350
NgoMIV G'CCGG_C
Cuts at: 0 1283 1635
Size: 1283 352
NheI G'CTAG_C
Cuts at: 0 1312 1635
Size: 1312 323
PflMI CCAn_nnn'nTGG
Cuts at: 0 654 959 1635
Size: 654 305 676
Fragments arranged by size:
676 654 305
PfoI T'CCnGG_A
Cuts at: 0 230 1635
Size: 230 1405
PpuMI rG'GwC_Cy
Cuts at: 0 1146 1635
Size: 1146 489
PshAI GACnn'nnGTC
Cuts at: 0 893 1532 1570 1635
Size: 893 639 38 65
Fragments arranged by size:
893 639 65 38
PspOMI G'GGCC_C
Cuts at: 0 365 1635
Size: 365 1270
PspXI vC'TCGA_Gb
Cuts at: 0 40 1635
Size: 40 1595
PstI C_TGCA'G
Cuts at: 0 584 1635
Size: 584 1051
PvuII CAG'CTG
Cuts at: 0 378 399 1635
Size: 378 21 1236
Fragments arranged by size:
1236 378 21
SacI G_AGCT'C
Cuts at: 0 1369 1635
Size: 1369 266
SanDI GG'GwC_CC
Cuts at: 0 1146 1635
Size: 1146 489
SapI GCTCTTCn'nnn_
Cuts at: 0 1367 1635
Size: 1367 268
ScaI AGT'ACT
Cuts at: 0 313 1635
Size: 313 1322
SfcI C'TryA_G
Cuts at: 0 580 1635
Size: 580 1055
SmaI CCC'GGG
Cuts at: 0 836 938 1570 1635
Size: 836 102 632 65
Fragments arranged by size:
836 632 102 65
SmlI C'TyrA_G
Cuts at: 0 40 298 1635
Size: 40 258 1337
Fragments arranged by size:
1337 258 40
SrfI GCCC'GGGC
Cuts at: 0 836 1635
Size: 836 799
StyI C'CwwG_G
Cuts at: 0 176 494 1358 1635
Size: 176 318 864 277
Fragments arranged by size:
864 318 277 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 670 1009 1635
Size: 670 339 626
Fragments arranged by size:
670 626 339
TatI w'GTAC_w
Cuts at: 0 311 1635
Size: 311 1324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 501 1635
Size: 501 1134
XhoI C'TCGA_G
Cuts at: 0 40 1635
Size: 40 1595
XmaI C'CCGG_G
Cuts at: 0 834 936 1568 1635
Size: 834 102 632 67
Fragments arranged by size:
834 632 102 67
Enzymes that do cut:
AcuI AfeI AleI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbsI BglI Bme1580I BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstEII
BstXI BstYI BtsI CspCI DraI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NaeI NgoMIV NheI PflMI PfoI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SanDI SapI ScaI SfcI
SmaI SmlI SrfI StyI TaqII TatI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgI BtgZI ClaI EagI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PsiI PsrI PvuI RsrII
SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TstI Tth111I
XbaI XmnI ZraI