Restriction map for
pFN21AE3933
(Linear) (Six-base) MAPSORT of: KIEE3933.seq Check: 496 from: 1 to: 663
KIEE3933.orf
With 182 enzymes: SgfI *
March 22, 2012 16:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 663
Size: 300 363
AfeI AGC'GCT
Cuts at: 0 53 663
Size: 53 610
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 663
Size: 628 35
AlwNI CAG_nnn'CTG
Cuts at: 0 143 379 663
Size: 143 236 284
Fragments arranged by size:
284 236 143
AvaI C'yCGr_G
Cuts at: 0 177 336 368 663
Size: 177 159 32 295
Fragments arranged by size:
295 177 159 32
BanI G'GyrC_C
Cuts at: 0 37 627 663
Size: 37 590 36
Fragments arranged by size:
590 37 36
BanII G_rGCy'C
Cuts at: 0 104 251 527 663
Size: 104 147 276 136
Fragments arranged by size:
276 147 136 104
BbeI G_GCGC'C
Cuts at: 0 41 663
Size: 41 622
BbvCI CC'TCA_GC
Cuts at: 0 541 663
Size: 541 122
BmgBI CAC'GTC
Cuts at: 0 83 663
Size: 83 580
BmrI ACTGGGnnnn_n'
Cuts at: 0 254 663
Size: 254 409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 663
Size: 583 80
Bpu10I CC'TnA_GC
Cuts at: 0 541 663
Size: 541 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 356 663
Size: 356 307
BsaHI Gr'CG_yC
Cuts at: 0 38 663
Size: 38 625
BsaWI w'CCGG_w
Cuts at: 0 581 663
Size: 581 82
BseYI C'CCAG_C
Cuts at: 0 167 663
Size: 167 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 663
Size: 529 134
BsiEI CG_ry'CG
Cuts at: 0 362 459 663
Size: 362 97 204
Fragments arranged by size:
362 204 97
BsiHKAI G_wGCw'C
Cuts at: 0 88 104 347 473 482 527 663
Size: 88 16 243 126 9 45 136
Fragments arranged by size:
243 136 126 88 45 16 9
Bsp1286I G_dGCh'C
Cuts at: 0 88 104 251 347 473 482 527 663
Size: 88 16 147 96 126 9 45 136
Fragments arranged by size:
147 136 126 96 88 45 16 9
BsrFI r'CCGG_y
Cuts at: 0 22 34 114 663
Size: 22 12 80 549
Fragments arranged by size:
549 80 22 12
BssHII G'CGCG_C
Cuts at: 0 209 253 663
Size: 209 44 410
Fragments arranged by size:
410 209 44
BstAPI GCAn_nnn'nTGC
Cuts at: 0 379 486 663
Size: 379 107 177
Fragments arranged by size:
379 177 107
BstXI CCAn_nnnn'nTGG
Cuts at: 0 447 663
Size: 447 216
BtgI C'CryG_G
Cuts at: 0 16 326 362 587 643 663
Size: 16 310 36 225 56 20
Fragments arranged by size:
310 225 56 36 20 16
BtsI GCAGTG_nn'
Cuts at: 0 223 244 663
Size: 223 21 419
Fragments arranged by size:
419 223 21
DraIII CAC_nnn'GTG
Cuts at: 0 486 663
Size: 486 177
EaeI y'GGCC_r
Cuts at: 0 132 296 359 663
Size: 132 164 63 304
Fragments arranged by size:
304 164 132 63
EagI C'GGCC_G
Cuts at: 0 359 663
Size: 359 304
EcoICRI GAG'CTC
Cuts at: 0 102 525 663
Size: 102 423 138
Fragments arranged by size:
423 138 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 583 663
Size: 300 283 80
Fragments arranged by size:
300 283 80
EcoO109I rG'GnC_Cy
Cuts at: 0 637 663
Size: 637 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 543 663
Size: 543 120
HaeII r_GCGC'y
Cuts at: 0 41 55 165 177 315 663
Size: 41 14 110 12 138 348
Fragments arranged by size:
348 138 110 41 14 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 334 366 425 457 663
Size: 103 32 199 32 59 32 206
Fragments arranged by size:
206 199 103 59 32 32 32
KasI G'GCGC_C
Cuts at: 0 37 663
Size: 37 626
MscI TGG'CCA
Cuts at: 0 298 663
Size: 298 365
MslI CAynn'nnrTG
Cuts at: 0 445 663
Size: 445 218
MspA1I CmG'CkG
Cuts at: 0 18 267 328 364 376 386 495 663
Size: 18 249 61 36 12 10 109 168
Fragments arranged by size:
249 168 109 61 36 18 12 10
NarI GG'CG_CC
Cuts at: 0 38 663
Size: 38 625
NotI GC'GGCC_GC
Cuts at: 0 359 663
Size: 359 304
PstI C_TGCA'G
Cuts at: 0 71 663
Size: 71 592
PvuII CAG'CTG
Cuts at: 0 267 663
Size: 267 396
RsrII CG'GwC_CG
Cuts at: 0 20 663
Size: 20 643
SacI G_AGCT'C
Cuts at: 0 104 527 663
Size: 104 423 136
Fragments arranged by size:
423 136 104
SacII CC_GC'GG
Cuts at: 0 19 329 365 663
Size: 19 310 36 298
Fragments arranged by size:
310 298 36 19
SfcI C'TryA_G
Cuts at: 0 67 663
Size: 67 596
SfoI GGC'GCC
Cuts at: 0 39 663
Size: 39 624
SmaI CCC'GGG
Cuts at: 0 179 338 370 663
Size: 179 159 32 293
Fragments arranged by size:
293 179 159 32
SrfI GCCC'GGGC
Cuts at: 0 179 370 663
Size: 179 191 293
Fragments arranged by size:
293 191 179
StyI C'CwwG_G
Cuts at: 0 518 663
Size: 518 145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 525 663
Size: 282 243 138
Fragments arranged by size:
282 243 138
XmaI C'CCGG_G
Cuts at: 0 177 336 368 663
Size: 177 159 32 295
Fragments arranged by size:
295 177 159 32
Enzymes that do cut:
AcuI AfeI AlfI AlwNI AvaI BanI BanII BbeI
BbvCI BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BssHII BstAPI
BstXI BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I KasI MscI MslI MspA1I
NarI NotI PstI PvuII RsrII SacI SacII SfcI
SfoI SmaI SrfI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmtI BplI BpuEI
BsaAI BsaBI BsaXI BseRI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssSI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI
NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmnI ZraI