(Linear) (Six-base) MAPSORT of: KIEE3932.seq  Check: 9887  from: 1  to: 576



KIEE3932.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:53  ..

AccI GT'mk_AC

Cuts at:      0     344     418     576 
   Size:        344      74     158

  Fragments arranged by size: 

                 344     158      74
 
AflIII A'CryG_T

Cuts at:      0      65     576 
   Size:         65     511
 
AvaI C'yCGr_G

Cuts at:      0     338     576 
   Size:        338     238
 
BanI G'GyrC_C

Cuts at:      0     152     576 
   Size:        152     424
 
BanII G_rGCy'C

Cuts at:      0     263     576 
   Size:        263     313
 
BbeI G_GCGC'C

Cuts at:      0     156     576 
   Size:        156     420
 
BbvCI CC'TCA_GC

Cuts at:      0     130     576 
   Size:        130     446
 
BlpI GC'TnA_GC

Cuts at:      0       5     576 
   Size:          5     571
 
Bme1580I G_kGCm'C

Cuts at:      0      40     169     576 
   Size:         40     129     407

  Fragments arranged by size: 

                 407     129      40
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     392     576 
   Size:        392     184
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     277     576 
   Size:        128     149     299

  Fragments arranged by size: 

                 299     149     128
 
Bpu10I CC'TnA_GC

Cuts at:      0     130     576 
   Size:        130     446
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     283     576 
   Size:         46     237     293

  Fragments arranged by size: 

                 293     237      46
 
BsaHI Gr'CG_yC

Cuts at:      0     153     576 
   Size:        153     423
 
BseYI C'CCAG_C

Cuts at:      0     365     436     576 
   Size:        365      71     140

  Fragments arranged by size: 

                 365     140      71
 
Bsp1286I G_dGCh'C

Cuts at:      0      40     169     263     576 
   Size:         40     129      94     313

  Fragments arranged by size: 

                 313     129      94      40
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      87     192     576 
   Size:         87     105     384

  Fragments arranged by size: 

                 384     105      87
 
Bsu36I CC'TnA_GG

Cuts at:      0     198     576 
   Size:        198     378
 
BtgI C'CryG_G

Cuts at:      0     224     576 
   Size:        224     352
 
BtsI GCAGTG_nn'

Cuts at:      0     230     576 
   Size:        230     346
 
EaeI y'GGCC_r

Cuts at:      0     500     576 
   Size:        500      76
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     241     576 
   Size:        241     335
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     277     576 
   Size:        128     149     299

  Fragments arranged by size: 

                 299     149     128
 
HaeII r_GCGC'y

Cuts at:      0     156     576 
   Size:        156     420
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     388     420     576 
   Size:        388      32     156

  Fragments arranged by size: 

                 388     156      32
 
KasI G'GCGC_C

Cuts at:      0     152     576 
   Size:        152     424
 
MslI CAynn'nnrTG

Cuts at:      0     235     576 
   Size:        235     341
 
MspA1I CmG'CkG

Cuts at:      0     291     576 
   Size:        291     285
 
NarI GG'CG_CC

Cuts at:      0     153     576 
   Size:        153     423
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     545     576 
   Size:        545      31
 
PfoI T'CCnGG_A

Cuts at:      0     275     576 
   Size:        275     301
 
PspXI vC'TCGA_Gb

Cuts at:      0     338     576 
   Size:        338     238
 
PvuII CAG'CTG

Cuts at:      0     291     576 
   Size:        291     285
 
SfoI GGC'GCC

Cuts at:      0     154     576 
   Size:        154     422
 
SmlI C'TyrA_G

Cuts at:      0      25     298     338     576 
   Size:         25     273      40     238

  Fragments arranged by size: 

                 273     238      40      25
 
StyI C'CwwG_G

Cuts at:      0     357     576 
   Size:        357     219
 
XhoI C'TCGA_G

Cuts at:      0     338     576 
   Size:        338     238
 
XmnI GAAnn'nnTTC

Cuts at:      0      55     576 
   Size:         55     521
 

 Enzymes that do cut:

     AccI   AflIII     AvaI     BanI    BanII     BbeI    BbvCI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaHI    BseYI Bsp1286I
    BstXI   Bsu36I     BtgI     BtsI     EaeI     EciI  Eco57MI    HaeII
    Hin4I     KasI     MslI   MspA1I     NarI    PflMI     PfoI    PspXI
    PvuII     SfoI     SmlI     StyI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI