Restriction map for
pFN21AE3931
(Linear) (Six-base) MAPSORT of: KIEE3931.seq Check: 9884 from: 1 to: 1635
KIEE3931.orf
With 182 enzymes: SgfI *
March 22, 2012 16:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1367 1635
Size: 1367 268
AccI GT'mk_AC
Cuts at: 0 464 1635
Size: 464 1171
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 670 740 1635
Size: 466 204 70 895
Fragments arranged by size:
895 466 204 70
AfeI AGC'GCT
Cuts at: 0 1147 1635
Size: 1147 488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1145 1635
Size: 1145 490
AlwNI CAG_nnn'CTG
Cuts at: 0 1374 1449 1635
Size: 1374 75 186
Fragments arranged by size:
1374 186 75
ApaI G_GGCC'C
Cuts at: 0 1179 1288 1635
Size: 1179 109 347
Fragments arranged by size:
1179 347 109
ApaLI G'TGCA_C
Cuts at: 0 1546 1635
Size: 1546 89
BanI G'GyrC_C
Cuts at: 0 525 1402 1508 1612 1635
Size: 525 877 106 104 23
Fragments arranged by size:
877 525 106 104 23
BanII G_rGCy'C
Cuts at: 0 1179 1288 1435 1459 1635
Size: 1179 109 147 24 176
Fragments arranged by size:
1179 176 147 109 24
BbeI G_GCGC'C
Cuts at: 0 1512 1635
Size: 1512 123
BbsI GAAGACnn'nnnn_
Cuts at: 0 17 143 1635
Size: 17 126 1492
Fragments arranged by size:
1492 126 17
BfrBI ATG'CAT
Cuts at: 0 759 1635
Size: 759 876
BlpI GC'TnA_GC
Cuts at: 0 1453 1635
Size: 1453 182
Bme1580I G_kGCm'C
Cuts at: 0 575 1179 1288 1405 1550 1615 1635
Size: 575 604 109 117 145 65 20
Fragments arranged by size:
604 575 145 117 109 65 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 1180 1215 1454 1635
Size: 1180 35 239 181
Fragments arranged by size:
1180 239 181 35
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 332 1635
Size: 332 1303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 251 619 865 1363 1635
Size: 235 16 368 246 498 272
Fragments arranged by size:
498 368 272 246 235 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 874 1635
Size: 874 761
BsaAI yAC'GTr
Cuts at: 0 576 1545 1635
Size: 576 969 90
Fragments arranged by size:
969 576 90
BsaHI Gr'CG_yC
Cuts at: 0 744 1509 1635
Size: 744 765 126
Fragments arranged by size:
765 744 126
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 49 58 133 136 145 181 184
Size: 46 3 9 75 3 9 36 3
Cuts at: 184 208 217 232 550 643 1314 1635
Size: 24 9 15 318 93 671 321
Fragments arranged by size:
671 321 318 93 75 46 36 24
15 9 9 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 92 767 771 818 1235 1364 1504 1635
Size: 92 675 4 47 417 129 140 131
Fragments arranged by size:
675 417 140 131 129 92 47 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 628 1635
Size: 565 63 1007
Fragments arranged by size:
1007 565 63
BsiEI CG_ry'CG
Cuts at: 0 1157 1197 1635
Size: 1157 40 438
Fragments arranged by size:
1157 438 40
BsiHKAI G_wGCw'C
Cuts at: 0 1435 1550 1604 1635
Size: 1435 115 54 31
Fragments arranged by size:
1435 115 54 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 751 1635
Size: 751 884
Bsp1286I G_dGCh'C
Cuts at: 0 575 1179 1288 1405 1435 1459 1550 1604
Size: 575 604 109 117 30 24 91 54
Cuts at: 1604 1615 1635
Size: 11 20
Fragments arranged by size:
604 575 117 109 91 54 30 24
20 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 347 1367 1635
Size: 74 273 1020 268
Fragments arranged by size:
1020 273 268 74
BsrBI CCG'CTC
Cuts at: 0 852 1116 1190 1635
Size: 852 264 74 445
Fragments arranged by size:
852 445 264 74
BsrFI r'CCGG_y
Cuts at: 0 1196 1349 1635
Size: 1196 153 286
Fragments arranged by size:
1196 286 153
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1374 1635
Size: 1374 261
BstXI CCAn_nnnn'nTGG
Cuts at: 0 483 941 1635
Size: 483 458 694
Fragments arranged by size:
694 483 458
BstYI r'GATC_y
Cuts at: 0 234 1635
Size: 234 1401
Bsu36I CC'TnA_GG
Cuts at: 0 178 1635
Size: 178 1457
BtgI C'CryG_G
Cuts at: 0 788 1301 1635
Size: 788 513 334
Fragments arranged by size:
788 513 334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1324 1635
Size: 1324 311
BtsI GCAGTG_nn'
Cuts at: 0 253 611 1635
Size: 253 358 1024
Fragments arranged by size:
1024 358 253
DraIII CAC_nnn'GTG
Cuts at: 0 522 1635
Size: 522 1113
EaeI y'GGCC_r
Cuts at: 0 347 1154 1194 1635
Size: 347 807 40 441
Fragments arranged by size:
807 441 347 40
EagI C'GGCC_G
Cuts at: 0 1154 1194 1635
Size: 1154 40 441
Fragments arranged by size:
1154 441 40
EarI CTCTTCn'nnn_
Cuts at: 0 47 224 457 464 646 716 908 1635
Size: 47 177 233 7 182 70 192 727
Fragments arranged by size:
727 233 192 182 177 70 47 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 319 336 1093 1635
Size: 319 17 757 542
Fragments arranged by size:
757 542 319 17
EcoICRI GAG'CTC
Cuts at: 0 1433 1635
Size: 1433 202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 251 466 619 670 740 865 1363
Size: 235 16 215 153 51 70 125 498
Cuts at: 1363 1635
Size: 272
Fragments arranged by size:
498 272 235 215 153 125 70 51
16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 392 1635
Size: 392 1243
EcoO109I rG'GnC_Cy
Cuts at: 0 174 1175 1285 1635
Size: 174 1001 110 350
Fragments arranged by size:
1001 350 174 110
HaeII r_GCGC'y
Cuts at: 0 1149 1479 1512 1635
Size: 1149 330 33 123
Fragments arranged by size:
1149 330 123 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 928 960 1005 1037 1306 1338 1635
Size: 928 32 45 32 269 32 297
Fragments arranged by size:
928 297 269 45 32 32 32
KasI G'GCGC_C
Cuts at: 0 1508 1635
Size: 1508 127
MscI TGG'CCA
Cuts at: 0 349 1635
Size: 349 1286
MslI CAynn'nnrTG
Cuts at: 0 481 971 1635
Size: 481 490 664
Fragments arranged by size:
664 490 481
MspA1I CmG'CkG
Cuts at: 0 92 345 679 771 790 924 1310 1383
Size: 92 253 334 92 19 134 386 73
Cuts at: 1383 1635
Size: 252
Fragments arranged by size:
386 334 253 252 134 92 92 73
19
NaeI GCC'GGC
Cuts at: 0 1198 1351 1635
Size: 1198 153 284
Fragments arranged by size:
1198 284 153
NarI GG'CG_CC
Cuts at: 0 1509 1635
Size: 1509 126
NgoMIV G'CCGG_C
Cuts at: 0 1196 1349 1635
Size: 1196 153 286
Fragments arranged by size:
1196 286 153
NsiI A_TGCA'T
Cuts at: 0 761 1635
Size: 761 874
NspI r_CATG'y
Cuts at: 0 293 1635
Size: 293 1342
PasI CC'CwG_GG
Cuts at: 0 1179 1288 1635
Size: 1179 109 347
Fragments arranged by size:
1179 347 109
PflMI CCAn_nnn'nTGG
Cuts at: 0 388 1635
Size: 388 1247
PfoI T'CCnGG_A
Cuts at: 0 499 881 1635
Size: 499 382 754
Fragments arranged by size:
754 499 382
PmlI CAC'GTG
Cuts at: 0 576 1545 1635
Size: 576 969 90
Fragments arranged by size:
969 576 90
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 731 763 1635
Size: 46 32 653 32 872
Fragments arranged by size:
872 653 46 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 174 1635
Size: 174 1461
PspOMI G'GGCC_C
Cuts at: 0 1175 1284 1635
Size: 1175 109 351
Fragments arranged by size:
1175 351 109
PstI C_TGCA'G
Cuts at: 0 344 569 929 1635
Size: 344 225 360 706
Fragments arranged by size:
706 360 344 225
PvuII CAG'CTG
Cuts at: 0 92 345 771 924 1635
Size: 92 253 426 153 711
Fragments arranged by size:
711 426 253 153 92
SacI G_AGCT'C
Cuts at: 0 1435 1635
Size: 1435 200
SacII CC_GC'GG
Cuts at: 0 791 1635
Size: 791 844
SapI GCTCTTCn'nnn_
Cuts at: 0 457 646 716 1635
Size: 457 189 70 919
Fragments arranged by size:
919 457 189 70
SfcI C'TryA_G
Cuts at: 0 340 565 925 1635
Size: 340 225 360 710
Fragments arranged by size:
710 360 340 225
SfoI GGC'GCC
Cuts at: 0 1510 1635
Size: 1510 125
StyI C'CwwG_G
Cuts at: 0 623 1471 1607 1635
Size: 623 848 136 28
Fragments arranged by size:
848 623 136 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 395 424 1234 1635
Size: 395 29 810 401
Fragments arranged by size:
810 401 395 29
TatI w'GTAC_w
Cuts at: 0 551 1635
Size: 551 1084
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 97 129 1378 1410 1635
Size: 97 32 1249 32 225
Fragments arranged by size:
1249 225 97 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 244 1635
Size: 244 1391
XbaI T'CTAG_A
Cuts at: 0 237 1635
Size: 237 1398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 1635
Size: 342 1293
XmnI GAAnn'nnTTC
Cuts at: 0 451 1635
Size: 451 1184
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AlwNI ApaI ApaLI
BanI BanII BbeI BbsI BfrBI BlpI Bme1580I BmrI
BplI BpmI BsaI BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuII SacI SacII SapI SfcI SfoI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BglI BglII BmgBI
BmtI Bpu10I BpuEI BsaBI BsaWI BsaXI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI NcoI NdeI NheI NotI NruI PacI
PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI XhoI XmaI ZraI