Restriction map for
pFN21AE3930
(Linear) (Six-base) MAPSORT of: KIEE3930.seq Check: 2977 from: 1 to: 1203
KIEE3930.orf
With 182 enzymes: SgfI *
March 22, 2012 16:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 222 1203
Size: 222 981
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 739 1203
Size: 711 28 464
Fragments arranged by size:
711 464 28
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 130 319 735 1203
Size: 130 189 416 468
Fragments arranged by size:
468 416 189 130
AlwNI CAG_nnn'CTG
Cuts at: 0 123 261 1138 1203
Size: 123 138 877 65
Fragments arranged by size:
877 138 123 65
ApaI G_GGCC'C
Cuts at: 0 102 215 1203
Size: 102 113 988
Fragments arranged by size:
988 113 102
AscI GG'CGCG_CC
Cuts at: 0 127 1203
Size: 127 1076
AvaI C'yCGr_G
Cuts at: 0 22 44 601 694 859 1203
Size: 22 22 557 93 165 344
Fragments arranged by size:
557 344 165 93 22 22
BamHI G'GATC_C
Cuts at: 0 965 1203
Size: 965 238
BanI G'GyrC_C
Cuts at: 0 18 27 162 605 649 698 1203
Size: 18 9 135 443 44 49 505
Fragments arranged by size:
505 443 135 49 44 18 9
BanII G_rGCy'C
Cuts at: 0 8 102 191 215 626 1203
Size: 8 94 89 24 411 577
Fragments arranged by size:
577 411 94 89 24 8
BbeI G_GCGC'C
Cuts at: 0 22 653 702 1203
Size: 22 631 49 501
Fragments arranged by size:
631 501 49 22
BbvCI CC'TCA_GC
Cuts at: 0 1185 1203
Size: 1185 18
BglI GCCn_nnn'nGGC
Cuts at: 0 1198 1203
Size: 1198 5
BlpI GC'TnA_GC
Cuts at: 0 467 1203
Size: 467 736
Bme1580I G_kGCm'C
Cuts at: 0 102 109 215 608 1203
Size: 102 7 106 393 595
Fragments arranged by size:
595 393 106 102 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1115 1203
Size: 205 910 88
Fragments arranged by size:
910 205 88
Bpu10I CC'TnA_GC
Cuts at: 0 1185 1203
Size: 1185 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 778 1203
Size: 331 447 425
Fragments arranged by size:
447 425 331
BsaI GGTCTCn'nnnn_
Cuts at: 0 47 412 983 1066 1203
Size: 47 365 571 83 137
Fragments arranged by size:
571 365 137 83 47
BsaHI Gr'CG_yC
Cuts at: 0 19 650 699 1203
Size: 19 631 49 504
Fragments arranged by size:
631 504 49 19
BsaWI w'CCGG_w
Cuts at: 0 881 1203
Size: 881 322
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 1203
Size: 67 30 1106
Fragments arranged by size:
1106 67 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 337 751 1126 1203
Size: 292 45 414 375 77
Fragments arranged by size:
414 375 292 77 45
BseYI C'CCAG_C
Cuts at: 0 35 500 1090 1203
Size: 35 465 590 113
Fragments arranged by size:
590 465 113 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 1203
Size: 1070 133
BsiEI CG_ry'CG
Cuts at: 0 71 155 638 1203
Size: 71 84 483 565
Fragments arranged by size:
565 483 84 71
BsiHKAI G_wGCw'C
Cuts at: 0 191 1203
Size: 191 1012
Bsp1286I G_dGCh'C
Cuts at: 0 8 102 109 191 215 608 626 1203
Size: 8 94 7 82 24 393 18 577
Fragments arranged by size:
577 393 94 82 24 18 8 7
BspEI T'CCGG_A
Cuts at: 0 881 1203
Size: 881 322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 222 407 1203
Size: 222 185 796
Fragments arranged by size:
796 222 185
BsrBI CCG'CTC
Cuts at: 0 762 1203
Size: 762 441
BsrFI r'CCGG_y
Cuts at: 0 246 640 1203
Size: 246 394 563
Fragments arranged by size:
563 394 246
BssHII G'CGCG_C
Cuts at: 0 127 306 327 329 507 1203
Size: 127 179 21 2 178 696
Fragments arranged by size:
696 179 178 127 21 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 1203
Size: 261 942
BstXI CCAn_nnnn'nTGG
Cuts at: 0 544 873 1203
Size: 544 329 330
Fragments arranged by size:
544 330 329
BstYI r'GATC_y
Cuts at: 0 120 749 965 1203
Size: 120 629 216 238
Fragments arranged by size:
629 238 216 120
Bsu36I CC'TnA_GG
Cuts at: 0 665 1203
Size: 665 538
BtgI C'CryG_G
Cuts at: 0 53 71 206 961 1203
Size: 53 18 135 755 242
Fragments arranged by size:
755 242 135 53 18
BtsI GCAGTG_nn'
Cuts at: 0 724 948 1203
Size: 724 224 255
Fragments arranged by size:
724 255 224
DrdI GACnn_nn'nnGTC
Cuts at: 0 841 1203
Size: 841 362
EaeI y'GGCC_r
Cuts at: 0 68 152 411 488 1203
Size: 68 84 259 77 715
Fragments arranged by size:
715 259 84 77 68
EagI C'GGCC_G
Cuts at: 0 68 152 1203
Size: 68 84 1051
Fragments arranged by size:
1051 84 68
EarI CTCTTCn'nnn_
Cuts at: 0 1103 1203
Size: 1103 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 265 691 1133 1203
Size: 265 426 442 70
Fragments arranged by size:
442 426 265 70
EcoICRI GAG'CTC
Cuts at: 0 189 1203
Size: 189 1014
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 711 739 1115 1203
Size: 205 506 28 376 88
Fragments arranged by size:
506 376 205 88 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 793 1203
Size: 793 410
EcoO109I rG'GnC_Cy
Cuts at: 0 98 99 211 669 843 1181 1203
Size: 98 1 112 458 174 338 22
Fragments arranged by size:
458 338 174 112 98 22 1
HaeII r_GCGC'y
Cuts at: 0 22 184 291 450 537 653 702 1203
Size: 22 162 107 159 87 116 49 501
Fragments arranged by size:
501 162 159 116 107 87 49 22
KasI G'GCGC_C
Cuts at: 0 18 649 698 1203
Size: 18 631 49 505
Fragments arranged by size:
631 505 49 18
MslI CAynn'nnrTG
Cuts at: 0 871 1203
Size: 871 332
MspA1I CmG'CkG
Cuts at: 0 55 73 369 441 504 819 891 1203
Size: 55 18 296 72 63 315 72 312
Fragments arranged by size:
315 312 296 72 72 63 55 18
NaeI GCC'GGC
Cuts at: 0 248 642 1203
Size: 248 394 561
Fragments arranged by size:
561 394 248
NarI GG'CG_CC
Cuts at: 0 19 650 699 1203
Size: 19 631 49 504
Fragments arranged by size:
631 504 49 19
NcoI C'CATG_G
Cuts at: 0 961 1203
Size: 961 242
NgoMIV G'CCGG_C
Cuts at: 0 246 640 1203
Size: 246 394 563
Fragments arranged by size:
563 394 246
PasI CC'CwG_GG
Cuts at: 0 102 655 1203
Size: 102 553 548
Fragments arranged by size:
553 548 102
PpuMI rG'GwC_Cy
Cuts at: 0 669 843 1203
Size: 669 174 360
Fragments arranged by size:
669 360 174
PspOMI G'GGCC_C
Cuts at: 0 98 211 1203
Size: 98 113 992
Fragments arranged by size:
992 113 98
PspXI vC'TCGA_Gb
Cuts at: 0 859 1203
Size: 859 344
PstI C_TGCA'G
Cuts at: 0 119 233 446 1203
Size: 119 114 213 757
Fragments arranged by size:
757 213 119 114
PvuII CAG'CTG
Cuts at: 0 441 504 1203
Size: 441 63 699
Fragments arranged by size:
699 441 63
RsrII CG'GwC_CG
Cuts at: 0 360 1203
Size: 360 843
SacI G_AGCT'C
Cuts at: 0 191 1203
Size: 191 1012
SacII CC_GC'GG
Cuts at: 0 56 74 1203
Size: 56 18 1129
Fragments arranged by size:
1129 56 18
SanDI GG'GwC_CC
Cuts at: 0 843 1203
Size: 843 360
SfcI C'TryA_G
Cuts at: 0 115 229 442 1203
Size: 115 114 213 761
Fragments arranged by size:
761 213 115 114
SfoI GGC'GCC
Cuts at: 0 20 651 700 1203
Size: 20 631 49 503
Fragments arranged by size:
631 503 49 20
SgrAI Cr'CCGG_yG
Cuts at: 0 640 1203
Size: 640 563
SmaI CCC'GGG
Cuts at: 0 24 603 1203
Size: 24 579 600
Fragments arranged by size:
600 579 24
SmlI C'TyrA_G
Cuts at: 0 346 793 859 1203
Size: 346 447 66 344
Fragments arranged by size:
447 346 344 66
SrfI GCCC'GGGC
Cuts at: 0 603 1203
Size: 603 600
StyI C'CwwG_G
Cuts at: 0 961 1203
Size: 961 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 377 436 1104 1203
Size: 377 59 668 99
Fragments arranged by size:
668 377 99 59
XhoI C'TCGA_G
Cuts at: 0 859 1203
Size: 859 344
XmaI C'CCGG_G
Cuts at: 0 22 601 1203
Size: 22 579 602
Fragments arranged by size:
602 579 22
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI AscI AvaI BamHI
BanI BanII BbeI BbvCI BglI BlpI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII KasI MslI MspA1I NaeI NarI NcoI NgoMIV
PasI PpuMI PspOMI PspXI PstI PvuII RsrII SacI
SacII SanDI SfcI SfoI SgrAI SmaI SmlI SrfI
StyI TaqII XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AseI AsiSI AvrII
BaeI BbsI BcgI BciVI BclI BfrBI BglII BmgBI
BmrI BmtI BplI BsaAI BsaBI BsiWI BsmI BsmBI
BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI SalI SapI SbfI ScaI
SexAI SfiI SgfI SnaBI SpeI SphI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XmnI ZraI