Restriction map for
pFN21AE3929
(Linear) (Six-base) MAPSORT of: KIEE3929.seq Check: 6065 from: 1 to: 1500
KIEE3929.orf
With 182 enzymes: SgfI *
March 22, 2012 16:52 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 1500
Size: 450 1050
AfeI AGC'GCT
Cuts at: 0 20 1500
Size: 20 1480
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 37 1500
Size: 37 1463
AlwNI CAG_nnn'CTG
Cuts at: 0 168 723 909 1500
Size: 168 555 186 591
Fragments arranged by size:
591 555 186 168
ApaI G_GGCC'C
Cuts at: 0 240 1500
Size: 240 1260
ApoI r'AATT_y
Cuts at: 0 1247 1500
Size: 1247 253
AvaI C'yCGr_G
Cuts at: 0 109 232 1500
Size: 109 123 1268
Fragments arranged by size:
1268 123 109
BanI G'GyrC_C
Cuts at: 0 819 1500
Size: 819 681
BanII G_rGCy'C
Cuts at: 0 67 101 240 434 957 1500
Size: 67 34 139 194 523 543
Fragments arranged by size:
543 523 194 139 67 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 66 521 1500
Size: 66 455 979
Fragments arranged by size:
979 455 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 129 163 1500
Size: 129 34 1337
Fragments arranged by size:
1337 129 34
BlpI GC'TnA_GC
Cuts at: 0 433 1100 1500
Size: 433 667 400
Fragments arranged by size:
667 433 400
Bme1580I G_kGCm'C
Cuts at: 0 240 254 1500
Size: 240 14 1246
Fragments arranged by size:
1246 240 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 426 1014 1500
Size: 426 588 486
Fragments arranged by size:
588 486 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 619 754 931 1500
Size: 283 336 135 177 569
Fragments arranged by size:
569 336 283 177 135
Bpu10I CC'TnA_GC
Cuts at: 0 949 1500
Size: 949 551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 1422 1500
Size: 1303 119 78
Fragments arranged by size:
1303 119 78
BsaBI GATnn'nnATC
Cuts at: 0 1012 1500
Size: 1012 488
BsaHI Gr'CG_yC
Cuts at: 0 638 1500
Size: 638 862
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 367 397 427 727 757 1500
Size: 31 30 306 30 30 300 30 743
Fragments arranged by size:
743 306 300 31 30 30 30 30
BseYI C'CCAG_C
Cuts at: 0 253 972 1500
Size: 253 719 528
Fragments arranged by size:
719 528 253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 1500
Size: 325 1175
BsiEI CG_ry'CG
Cuts at: 0 312 360 1287 1500
Size: 312 48 927 213
Fragments arranged by size:
927 312 213 48
BsiHKAI G_wGCw'C
Cuts at: 0 155 434 1500
Size: 155 279 1066
Fragments arranged by size:
1066 279 155
BsmI GAATG_Cn'
Cuts at: 0 1109 1500
Size: 1109 391
Bsp1286I G_dGCh'C
Cuts at: 0 67 101 155 240 254 434 957 1500
Size: 67 34 54 85 14 180 523 543
Fragments arranged by size:
543 523 180 85 67 54 34 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 722 1500
Size: 722 778
BsrFI r'CCGG_y
Cuts at: 0 355 1500
Size: 355 1145
BssHII G'CGCG_C
Cuts at: 0 386 1500
Size: 386 1114
BstXI CCAn_nnnn'nTGG
Cuts at: 0 261 288 1500
Size: 261 27 1212
Fragments arranged by size:
1212 261 27
BstYI r'GATC_y
Cuts at: 0 407 610 1500
Size: 407 203 890
Fragments arranged by size:
890 407 203
BtgI C'CryG_G
Cuts at: 0 206 287 1061 1500
Size: 206 81 774 439
Fragments arranged by size:
774 439 206 81
ClaI AT'CG_AT
Cuts at: 0 425 440 1500
Size: 425 15 1060
Fragments arranged by size:
1060 425 15
DraIII CAC_nnn'GTG
Cuts at: 0 1022 1500
Size: 1022 478
EaeI y'GGCC_r
Cuts at: 0 203 242 357 1500
Size: 203 39 115 1143
Fragments arranged by size:
1143 203 115 39
EagI C'GGCC_G
Cuts at: 0 357 1500
Size: 357 1143
EarI CTCTTCn'nnn_
Cuts at: 0 872 1500
Size: 872 628
EcoICRI GAG'CTC
Cuts at: 0 432 1500
Size: 432 1068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 450 619 754 931 1500
Size: 283 167 169 135 177 569
Fragments arranged by size:
569 283 177 169 167 135
EcoNI CCTnn'n_nnAGG
Cuts at: 0 450 814 1500
Size: 450 364 686
Fragments arranged by size:
686 450 364
EcoO109I rG'GnC_Cy
Cuts at: 0 236 237 559 1500
Size: 236 1 322 941
Fragments arranged by size:
941 322 236 1
EcoRI G'AATT_C
Cuts at: 0 1247 1500
Size: 1247 253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1154 1500
Size: 1154 346
FspI TGC'GCA
Cuts at: 0 961 1500
Size: 961 539
HaeII r_GCGC'y
Cuts at: 0 22 109 169 1500
Size: 22 87 60 1331
Fragments arranged by size:
1331 87 60 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 301 333 1276 1308 1500
Size: 31 32 238 32 943 32 192
Fragments arranged by size:
943 238 192 32 32 32 31
HincII GTy'rAC
Cuts at: 0 129 529 1398 1500
Size: 129 400 869 102
Fragments arranged by size:
869 400 129 102
HindIII A'AGCT_T
Cuts at: 0 1357 1500
Size: 1357 143
HpaI GTT'AAC
Cuts at: 0 1398 1500
Size: 1398 102
MslI CAynn'nnrTG
Cuts at: 0 536 676 1149 1165 1432 1500
Size: 536 140 473 16 267 68
Fragments arranged by size:
536 473 267 140 68 16
MspA1I CmG'CkG
Cuts at: 0 11 36 44 381 622 694 720 1330
Size: 11 25 8 337 241 72 26 610
Cuts at: 1330 1478 1500
Size: 148 22
Fragments arranged by size:
610 337 241 148 72 26 25 22
11 8
NspI r_CATG'y
Cuts at: 0 370 966 1500
Size: 370 596 534
Fragments arranged by size:
596 534 370
PasI CC'CwG_GG
Cuts at: 0 60 1500
Size: 60 1440
PfoI T'CCnGG_A
Cuts at: 0 596 731 1500
Size: 596 135 769
Fragments arranged by size:
769 596 135
PpuMI rG'GwC_Cy
Cuts at: 0 559 1500
Size: 559 941
PshAI GACnn'nnGTC
Cuts at: 0 800 1500
Size: 800 700
PspOMI G'GGCC_C
Cuts at: 0 236 1500
Size: 236 1264
PstI C_TGCA'G
Cuts at: 0 82 404 454 719 1500
Size: 82 322 50 265 781
Fragments arranged by size:
781 322 265 82 50
PvuI CG_AT'CG
Cuts at: 0 1287 1500
Size: 1287 213
PvuII CAG'CTG
Cuts at: 0 381 622 694 720 1330 1500
Size: 381 241 72 26 610 170
Fragments arranged by size:
610 381 241 170 72 26
SacI G_AGCT'C
Cuts at: 0 434 1500
Size: 434 1066
SbfI CC_TGCA'GG
Cuts at: 0 404 1500
Size: 404 1096
ScaI AGT'ACT
Cuts at: 0 301 415 1467 1500
Size: 301 114 1052 33
Fragments arranged by size:
1052 301 114 33
SfcI C'TryA_G
Cuts at: 0 78 400 450 715 1085 1500
Size: 78 322 50 265 370 415
Fragments arranged by size:
415 370 322 265 78 50
SmaI CCC'GGG
Cuts at: 0 234 1500
Size: 234 1266
SmlI C'TyrA_G
Cuts at: 0 1318 1401 1500
Size: 1318 83 99
Fragments arranged by size:
1318 99 83
SphI G_CATG'C
Cuts at: 0 966 1500
Size: 966 534
StyI C'CwwG_G
Cuts at: 0 101 192 555 1002 1500
Size: 101 91 363 447 498
Fragments arranged by size:
498 447 363 101 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1033 1500
Size: 1033 467
TatI w'GTAC_w
Cuts at: 0 299 413 1465 1500
Size: 299 114 1052 35
Fragments arranged by size:
1052 299 114 35
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 367 399 727 759 1500
Size: 367 32 328 32 741
Fragments arranged by size:
741 367 328 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 563 1500
Size: 563 937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 654 675 906 1500
Size: 654 21 231 594
Fragments arranged by size:
654 594 231 21
XmaI C'CCGG_G
Cuts at: 0 232 1500
Size: 232 1268
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BcgI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BssHII BstXI BstYI BtgI
ClaI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI MslI MspA1I NspI PasI PfoI PpuMI PshAI
PspOMI PstI PvuI PvuII SacI SbfI ScaI SfcI
SmaI SmlI SphI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BplI BsaI BsaAI BsaWI BseRI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI CspCI
DraI DrdI EciI EcoRV FseI FspAI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PsiI PspXI PsrI RsrII SacII
SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI
XmnI ZraI