Restriction map for
pFN21AE3928
(Linear) (Six-base) MAPSORT of: KIEE3928.seq Check: 8126 from: 1 to: 1188
KIEE3928.orf
With 182 enzymes: SgfI *
March 22, 2012 16:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 674 1188
Size: 674 514
AatII G_ACGT'C
Cuts at: 0 235 1188
Size: 235 953
AccI GT'mk_AC
Cuts at: 0 865 1071 1188
Size: 865 206 117
Fragments arranged by size:
865 206 117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1115 1188
Size: 874 241 73
Fragments arranged by size:
874 241 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 542 1188
Size: 542 646
AlwNI CAG_nnn'CTG
Cuts at: 0 1023 1188
Size: 1023 165
ApoI r'AATT_y
Cuts at: 0 70 405 1188
Size: 70 335 783
Fragments arranged by size:
783 335 70
AvaI C'yCGr_G
Cuts at: 0 463 564 1188
Size: 463 101 624
Fragments arranged by size:
624 463 101
BanII G_rGCy'C
Cuts at: 0 965 1188
Size: 965 223
BciVI GTATCCnnnnn_n'
Cuts at: 0 604 919 1188
Size: 604 315 269
Fragments arranged by size:
604 315 269
BfrBI ATG'CAT
Cuts at: 0 257 1188
Size: 257 931
Bme1580I G_kGCm'C
Cuts at: 0 687 1188
Size: 687 501
BmrI ACTGGGnnnn_n'
Cuts at: 0 373 643 1188
Size: 373 270 545
Fragments arranged by size:
545 373 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 1027 1188
Size: 699 328 161
Fragments arranged by size:
699 328 161
Bpu10I CC'TnA_GC
Cuts at: 0 326 1188
Size: 326 862
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 1188
Size: 987 201
BsaI GGTCTCn'nnnn_
Cuts at: 0 83 837 1188
Size: 83 754 351
Fragments arranged by size:
754 351 83
BsaHI Gr'CG_yC
Cuts at: 0 232 1188
Size: 232 956
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 994 1188
Size: 34 960 194
Fragments arranged by size:
960 194 34
BsiHKAI G_wGCw'C
Cuts at: 0 909 1188
Size: 909 279
BsmI GAATG_Cn'
Cuts at: 0 417 1188
Size: 417 771
Bsp1286I G_dGCh'C
Cuts at: 0 687 909 965 1188
Size: 687 222 56 223
Fragments arranged by size:
687 223 222 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 674 1188
Size: 674 514
BsrBI CCG'CTC
Cuts at: 0 1001 1188
Size: 1001 187
BsrDI GCAATG_nn'
Cuts at: 0 428 1188
Size: 428 760
BstAPI GCAn_nnn'nTGC
Cuts at: 0 913 1188
Size: 913 275
BstBI TT'CG_AA
Cuts at: 0 49 1188
Size: 49 1139
BstXI CCAn_nnnn'nTGG
Cuts at: 0 629 1188
Size: 629 559
BtgI C'CryG_G
Cuts at: 0 13 497 597 1188
Size: 13 484 100 591
Fragments arranged by size:
591 484 100 13
ClaI AT'CG_AT
Cuts at: 0 1064 1188
Size: 1064 124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 809 844 1188
Size: 809 35 344
Fragments arranged by size:
809 344 35
EarI CTCTTCn'nnn_
Cuts at: 0 617 1115 1188
Size: 617 498 73
Fragments arranged by size:
617 498 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 874 1027 1115 1188
Size: 699 175 153 88 73
Fragments arranged by size:
699 175 153 88 73
EcoO109I rG'GnC_Cy
Cuts at: 0 893 1188
Size: 893 295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 459 491 817 849 1188
Size: 459 32 326 32 339
Fragments arranged by size:
459 339 326 32 32
HincII GTy'rAC
Cuts at: 0 1072 1188
Size: 1072 116
HindIII A'AGCT_T
Cuts at: 0 295 1188
Size: 295 893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 1188
Size: 144 1044
MslI CAynn'nnrTG
Cuts at: 0 649 1188
Size: 649 539
MspA1I CmG'CkG
Cuts at: 0 522 607 1188
Size: 522 85 581
Fragments arranged by size:
581 522 85
NcoI C'CATG_G
Cuts at: 0 597 1188
Size: 597 591
NsiI A_TGCA'T
Cuts at: 0 259 1188
Size: 259 929
NspI r_CATG'y
Cuts at: 0 516 1188
Size: 516 672
PfoI T'CCnGG_A
Cuts at: 0 1022 1188
Size: 1022 166
PshAI GACnn'nnGTC
Cuts at: 0 231 1188
Size: 231 957
PstI C_TGCA'G
Cuts at: 0 226 521 1188
Size: 226 295 667
Fragments arranged by size:
667 295 226
PvuII CAG'CTG
Cuts at: 0 522 607 1188
Size: 522 85 581
Fragments arranged by size:
581 522 85
SalI G'TCGA_C
Cuts at: 0 1070 1188
Size: 1070 118
SapI GCTCTTCn'nnn_
Cuts at: 0 617 1188
Size: 617 571
SexAI A'CCwGG_T
Cuts at: 0 954 1188
Size: 954 234
SfcI C'TryA_G
Cuts at: 0 103 222 517 866 930 1188
Size: 103 119 295 349 64 258
Fragments arranged by size:
349 295 258 119 103 64
SmlI C'TyrA_G
Cuts at: 0 564 1002 1188
Size: 564 438 186
Fragments arranged by size:
564 438 186
SspI AAT'ATT
Cuts at: 0 487 1188
Size: 487 701
StyI C'CwwG_G
Cuts at: 0 597 1188
Size: 597 591
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 392 1188
Size: 392 796
TatI w'GTAC_w
Cuts at: 0 183 1188
Size: 183 1005
XhoI C'TCGA_G
Cuts at: 0 564 1188
Size: 564 624
XmnI GAAnn'nnTTC
Cuts at: 0 1018 1188
Size: 1018 170
ZraI GAC'GTC
Cuts at: 0 233 1188
Size: 233 955
Enzymes that do cut:
AarI AatII AccI AcuI AlfI AlwNI ApoI AvaI
BanII BciVI BfrBI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BseRI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BstAPI BstBI BstXI BtgI ClaI CspCI EarI
Eco57MI EcoO109I Hin4I HincII HindIII MmeI MslI MspA1I
NcoI NsiI NspI PfoI PshAI PstI PvuII SalI
SapI SexAI SfcI SmlI SspI StyI TaqII TatI
XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BclI BglI
BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaWI
BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XmaI