(Linear) (Six-base) MAPSORT of: KIEE3927.seq  Check: 3674  from: 1  to: 1305



KIEE3927.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:52  ..

AclI AA'CG_TT

Cuts at:      0     145    1305 
   Size:        145    1160
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239    1153    1305 
   Size:        239     914     152

  Fragments arranged by size: 

                 914     239     152
 
AflII C'TTAA_G

Cuts at:      0     985    1305 
   Size:        985     320
 
AflIII A'CryG_T

Cuts at:      0     171    1305 
   Size:        171    1134
 
ApoI r'AATT_y

Cuts at:      0     224    1305 
   Size:        224    1081
 
BanII G_rGCy'C

Cuts at:      0     167     674    1305 
   Size:        167     507     631

  Fragments arranged by size: 

                 631     507     167
 
BclI T'GATC_A

Cuts at:      0     579    1305 
   Size:        579     726
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     822    1305 
   Size:        822     483
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572    1305 
   Size:        572     733
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23    1305 
   Size:         23    1282
 
BsiHKAI G_wGCw'C

Cuts at:      0     167     674    1305 
   Size:        167     507     631

  Fragments arranged by size: 

                 631     507     167
 
BsmI GAATG_Cn'

Cuts at:      0     199    1305 
   Size:        199    1106
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     674    1305 
   Size:        167     507     631

  Fragments arranged by size: 

                 631     507     167
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     943    1305 
   Size:        943     362
 
BstYI r'GATC_y

Cuts at:      0     566     858    1305 
   Size:        566     292     447

  Fragments arranged by size: 

                 566     447     292
 
BtgI C'CryG_G

Cuts at:      0     716    1305 
   Size:        716     589
 
DraI TTT'AAA

Cuts at:      0     230    1305 
   Size:        230    1075
 
EaeI y'GGCC_r

Cuts at:      0    1188    1305 
   Size:       1188     117
 
EcoICRI GAG'CTC

Cuts at:      0     165     672    1305 
   Size:        165     507     633

  Fragments arranged by size: 

                 633     507     165
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     572    1153    1305 
   Size:        239     333     581     152

  Fragments arranged by size: 

                 581     333     239     152
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     424    1305 
   Size:        424     881
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     789     821    1029    1061    1140    1172    1305 
   Size:        789      32     208      32      79      32     133

  Fragments arranged by size: 

                 789     208     133      79      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1178    1305 
   Size:       1178     127
 
HpaI GTT'AAC

Cuts at:      0    1178    1305 
   Size:       1178     127
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1150    1305 
   Size:       1150     155
 
MscI TGG'CCA

Cuts at:      0    1190    1305 
   Size:       1190     115
 
MslI CAynn'nnrTG

Cuts at:      0     893    1305 
   Size:        893     412
 
MspA1I CmG'CkG

Cuts at:      0     334     601    1173    1305 
   Size:        334     267     572     132

  Fragments arranged by size: 

                 572     334     267     132
 
NruI TCG'CGA

Cuts at:      0      32    1305 
   Size:         32    1273
 
NspI r_CATG'y

Cuts at:      0     175    1305 
   Size:        175    1130
 
PciI A'CATG_T

Cuts at:      0     171    1305 
   Size:        171    1134
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     505    1305 
   Size:        505     800
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     350     382    1305 
   Size:        350      32     923

  Fragments arranged by size: 

                 923     350      32
 
PvuII CAG'CTG

Cuts at:      0     334     601    1173    1305 
   Size:        334     267     572     132

  Fragments arranged by size: 

                 572     334     267     132
 
SacI G_AGCT'C

Cuts at:      0     167     674    1305 
   Size:        167     507     631

  Fragments arranged by size: 

                 631     507     167
 
ScaI AGT'ACT

Cuts at:      0     689    1305 
   Size:        689     616
 
SfcI C'TryA_G

Cuts at:      0     841    1305 
   Size:        841     464
 
SmlI C'TyrA_G

Cuts at:      0      38     985    1305 
   Size:         38     947     320

  Fragments arranged by size: 

                 947     320      38
 
SspI AAT'ATT

Cuts at:      0     706     768    1094    1137    1305 
   Size:        706      62     326      43     168

  Fragments arranged by size: 

                 706     326     168      62      43
 
StuI AGG'CCT

Cuts at:      0     878    1305 
   Size:        878     427
 
StyI C'CwwG_G

Cuts at:      0    1053    1305 
   Size:       1053     252
 
TatI w'GTAC_w

Cuts at:      0     687    1305 
   Size:        687     618
 
XbaI T'CTAG_A

Cuts at:      0     369    1236    1305 
   Size:        369     867      69

  Fragments arranged by size: 

                 867     369      69
 
XmnI GAAnn'nnTTC

Cuts at:      0     491    1305 
   Size:        491     814
 

 Enzymes that do cut:

     AclI     AcuI    AflII   AflIII     ApoI    BanII     BclI     BmrI
     BpmI    BpuEI  BsiHKAI     BsmI Bsp1286I   BstAPI    BstYI     BtgI
     DraI     EaeI  EcoICRI  Eco57MI     FalI    Hin4I   HincII     HpaI
     MmeI     MscI     MslI   MspA1I     NruI     NspI     PciI    PflMI
     PsrI    PvuII     SacI     ScaI     SfcI     SmlI     SspI     StuI
     StyI     TatI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EagI     EarI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NsiI     PacI     PasI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SwaI    TaqII     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI