Restriction map for
pFN21AE3922
(Linear) (Six-base) MAPSORT of: KIEE3922.seq Check: 3713 from: 1 to: 2727
KIEE3922.orf
With 182 enzymes: SgfI *
March 22, 2012 16:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 2727
Size: 908 1819
AfeI AGC'GCT
Cuts at: 0 363 2727
Size: 363 2364
AflIII A'CryG_T
Cuts at: 0 186 812 1379 2727
Size: 186 626 567 1348
Fragments arranged by size:
1348 626 567 186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1760 2650 2727
Size: 1760 890 77
Fragments arranged by size:
1760 890 77
AlwNI CAG_nnn'CTG
Cuts at: 0 1237 2727
Size: 1237 1490
ApaLI G'TGCA_C
Cuts at: 0 1633 2727
Size: 1633 1094
ApoI r'AATT_y
Cuts at: 0 153 160 1432 2067 2727
Size: 153 7 1272 635 660
Fragments arranged by size:
1272 660 635 153 7
AseI AT'TA_AT
Cuts at: 0 100 742 2727
Size: 100 642 1985
Fragments arranged by size:
1985 642 100
AvaI C'yCGr_G
Cuts at: 0 70 756 2727
Size: 70 686 1971
Fragments arranged by size:
1971 686 70
BanI G'GyrC_C
Cuts at: 0 2160 2727
Size: 2160 567
BbsI GAAGACnn'nnnn_
Cuts at: 0 803 2468 2727
Size: 803 1665 259
Fragments arranged by size:
1665 803 259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2052 2086 2727
Size: 2052 34 641
Fragments arranged by size:
2052 641 34
BclI T'GATC_A
Cuts at: 0 120 1905 2727
Size: 120 1785 822
Fragments arranged by size:
1785 822 120
BfrBI ATG'CAT
Cuts at: 0 178 1548 1840 2627 2727
Size: 178 1370 292 787 100
Fragments arranged by size:
1370 787 292 178 100
BglII A'GATC_T
Cuts at: 0 1937 2727
Size: 1937 790
BlpI GC'TnA_GC
Cuts at: 0 919 2727
Size: 919 1808
Bme1580I G_kGCm'C
Cuts at: 0 1637 2727
Size: 1637 1090
BmgBI CAC'GTC
Cuts at: 0 187 987 2727
Size: 187 800 1740
Fragments arranged by size:
1740 800 187
BmrI ACTGGGnnnn_n'
Cuts at: 0 1007 1845 2727
Size: 1007 838 882
Fragments arranged by size:
1007 882 838
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2506 2727
Size: 2506 221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1214 2727
Size: 850 364 1513
Fragments arranged by size:
1513 850 364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2456 2623 2656 2727
Size: 2456 167 33 71
Fragments arranged by size:
2456 167 71 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 823 853 1683 1713 2727
Size: 823 30 830 30 1014
Fragments arranged by size:
1014 830 823 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 671 683 2491 2727
Size: 671 12 1808 236
Fragments arranged by size:
1808 671 236 12
BseYI C'CCAG_C
Cuts at: 0 1930 2727
Size: 1930 797
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 559 1334 2727
Size: 472 87 775 1393
Fragments arranged by size:
1393 775 472 87
BsiHKAI G_wGCw'C
Cuts at: 0 1637 2727
Size: 1637 1090
BsmI GAATG_Cn'
Cuts at: 0 178 241 1605 2727
Size: 178 63 1364 1122
Fragments arranged by size:
1364 1122 178 63
Bsp1286I G_dGCh'C
Cuts at: 0 1637 2727
Size: 1637 1090
BsrDI GCAATG_nn'
Cuts at: 0 209 2080 2156 2727
Size: 209 1871 76 571
Fragments arranged by size:
1871 571 209 76
BsrFI r'CCGG_y
Cuts at: 0 1349 2727
Size: 1349 1378
BsrGI T'GTAC_A
Cuts at: 0 295 708 2727
Size: 295 413 2019
Fragments arranged by size:
2019 413 295
BssHII G'CGCG_C
Cuts at: 0 52 2727
Size: 52 2675
BstAPI GCAn_nnn'nTGC
Cuts at: 0 546 1723 2727
Size: 546 1177 1004
Fragments arranged by size:
1177 1004 546
BstBI TT'CG_AA
Cuts at: 0 993 2241 2727
Size: 993 1248 486
Fragments arranged by size:
1248 993 486
BstYI r'GATC_y
Cuts at: 0 641 1937 2427 2727
Size: 641 1296 490 300
Fragments arranged by size:
1296 641 490 300
BtgI C'CryG_G
Cuts at: 0 59 485 1758 2727
Size: 59 426 1273 969
Fragments arranged by size:
1273 969 426 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 25 2727
Size: 25 2702
BtsI GCAGTG_nn'
Cuts at: 0 499 547 2727
Size: 499 48 2180
Fragments arranged by size:
2180 499 48
DraI TTT'AAA
Cuts at: 0 1460 1572 2727
Size: 1460 112 1155
Fragments arranged by size:
1460 1155 112
DraIII CAC_nnn'GTG
Cuts at: 0 2530 2727
Size: 2530 197
EarI CTCTTCn'nnn_
Cuts at: 0 946 2150 2727
Size: 946 1204 577
Fragments arranged by size:
1204 946 577
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 441 2727
Size: 441 2286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 908 1214 2727
Size: 850 58 306 1513
Fragments arranged by size:
1513 850 306 58
EcoO109I rG'GnC_Cy
Cuts at: 0 1234 2520 2727
Size: 1234 1286 207
Fragments arranged by size:
1286 1234 207
EcoRV GAT'ATC
Cuts at: 0 1659 2727
Size: 1659 1068
HaeII r_GCGC'y
Cuts at: 0 365 2727
Size: 365 2362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 1404 1436 1519 1551 1795 1812
Size: 823 32 549 32 83 32 244 17
Cuts at: 1812 1827 1844 2026 2058 2727
Size: 15 17 182 32 669
Fragments arranged by size:
823 669 549 244 182 83 32 32
32 32 17 17 15
HincII GTy'rAC
Cuts at: 0 372 2727
Size: 372 2355
HindIII A'AGCT_T
Cuts at: 0 198 1370 2727
Size: 198 1172 1357
Fragments arranged by size:
1357 1172 198
MslI CAynn'nnrTG
Cuts at: 0 461 2632 2727
Size: 461 2171 95
Fragments arranged by size:
2171 461 95
MspA1I CmG'CkG
Cuts at: 0 1858 1968 2727
Size: 1858 110 759
Fragments arranged by size:
1858 759 110
NcoI C'CATG_G
Cuts at: 0 485 1758 2727
Size: 485 1273 969
Fragments arranged by size:
1273 969 485
NdeI CA'TA_TG
Cuts at: 0 2294 2727
Size: 2294 433
NsiI A_TGCA'T
Cuts at: 0 180 1550 1842 2629 2727
Size: 180 1370 292 787 98
Fragments arranged by size:
1370 787 292 180 98
NspI r_CATG'y
Cuts at: 0 460 508 816 1383 2364 2627 2637 2727
Size: 460 48 308 567 981 263 10 90
Fragments arranged by size:
981 567 460 308 263 90 48 10
PciI A'CATG_T
Cuts at: 0 812 1379 2727
Size: 812 567 1348
Fragments arranged by size:
1348 812 567
PflMI CCAn_nnn'nTGG
Cuts at: 0 1237 2037 2530 2727
Size: 1237 800 493 197
Fragments arranged by size:
1237 800 493 197
PfoI T'CCnGG_A
Cuts at: 0 1230 1404 2727
Size: 1230 174 1323
Fragments arranged by size:
1323 1230 174
PpuMI rG'GwC_Cy
Cuts at: 0 1234 2520 2727
Size: 1234 1286 207
Fragments arranged by size:
1286 1234 207
PvuII CAG'CTG
Cuts at: 0 1858 1968 2727
Size: 1858 110 759
Fragments arranged by size:
1858 759 110
SanDI GG'GwC_CC
Cuts at: 0 2520 2727
Size: 2520 207
SapI GCTCTTCn'nnn_
Cuts at: 0 946 2727
Size: 946 1781
SmlI C'TyrA_G
Cuts at: 0 2471 2638 2671 2727
Size: 2471 167 33 56
Fragments arranged by size:
2471 167 56 33
SphI G_CATG'C
Cuts at: 0 2364 2727
Size: 2364 363
SspI AAT'ATT
Cuts at: 0 1977 2727
Size: 1977 750
StuI AGG'CCT
Cuts at: 0 392 2727
Size: 392 2335
StyI C'CwwG_G
Cuts at: 0 485 1758 2727
Size: 485 1273 969
Fragments arranged by size:
1273 969 485
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2539 2727
Size: 2539 188
TatI w'GTAC_w
Cuts at: 0 295 602 708 2727
Size: 295 307 106 2019
Fragments arranged by size:
2019 307 295 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1683 1715 2727
Size: 1683 32 1012
Fragments arranged by size:
1683 1012 32
XbaI T'CTAG_A
Cuts at: 0 2109 2727
Size: 2109 618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1858 2438 2727
Size: 1858 580 289
Fragments arranged by size:
1858 580 289
XmnI GAAnn'nnTTC
Cuts at: 0 1413 2727
Size: 1413 1314
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI ApaLI ApoI AseI
AvaI BanI BbsI BcgI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII EarI EciI Eco57MI EcoO109I EcoRV HaeII Hin4I
HincII HindIII MslI MspA1I NcoI NdeI NsiI NspI
PciI PflMI PfoI PpuMI PvuII SanDI SapI SmlI
SphI SspI StuI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI ApaI AscI AsiSI AvrII BaeI BamHI
BanII BbeI BbvCI BciVI BglI BmtI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BssSI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DrdI EaeI EagI EcoICRI EcoNI EcoRI
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI
XmaI ZraI