Restriction map for
pFN21AE3919
(Linear) (Six-base) MAPSORT of: KIEE3919.seq Check: 3853 from: 1 to: 2121
KIEE3919.orf
With 182 enzymes: SgfI *
March 22, 2012 16:50 ..
AccI GT'mk_AC
Cuts at: 0 640 2121
Size: 640 1481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1008 1858 2121
Size: 446 562 850 263
Fragments arranged by size:
850 562 446 263
AfeI AGC'GCT
Cuts at: 0 1507 2121
Size: 1507 614
AflII C'TTAA_G
Cuts at: 0 1903 2121
Size: 1903 218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 153 1059 1483 2121
Size: 153 906 424 638
Fragments arranged by size:
906 638 424 153
AlwNI CAG_nnn'CTG
Cuts at: 0 728 948 2121
Size: 728 220 1173
Fragments arranged by size:
1173 728 220
ApaLI G'TGCA_C
Cuts at: 0 1154 2121
Size: 1154 967
ApoI r'AATT_y
Cuts at: 0 678 1255 1368 1452 2121
Size: 678 577 113 84 669
Fragments arranged by size:
678 669 577 113 84
AseI AT'TA_AT
Cuts at: 0 1070 2121
Size: 1070 1051
AvaI C'yCGr_G
Cuts at: 0 810 2121
Size: 810 1311
BamHI G'GATC_C
Cuts at: 0 1898 2121
Size: 1898 223
BanII G_rGCy'C
Cuts at: 0 1188 2121
Size: 1188 933
BbsI GAAGACnn'nnnn_
Cuts at: 0 200 685 2114 2121
Size: 200 485 1429 7
Fragments arranged by size:
1429 485 200 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 224 2121
Size: 224 1897
BfrBI ATG'CAT
Cuts at: 0 1059 1246 2121
Size: 1059 187 875
Fragments arranged by size:
1059 875 187
BglII A'GATC_T
Cuts at: 0 715 1605 2121
Size: 715 890 516
Fragments arranged by size:
890 715 516
Bme1580I G_kGCm'C
Cuts at: 0 1158 2121
Size: 1158 963
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1548 1885 2121
Size: 1548 337 236
Fragments arranged by size:
1548 337 236
BsaWI w'CCGG_w
Cuts at: 0 6 1895 2121
Size: 6 1889 226
Fragments arranged by size:
1889 226 6
BseYI C'CCAG_C
Cuts at: 0 1688 2121
Size: 1688 433
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1029 1801 1902 2121
Size: 984 45 772 101 219
Fragments arranged by size:
984 772 219 101 45
BsiHKAI G_wGCw'C
Cuts at: 0 1158 1188 2121
Size: 1158 30 933
Fragments arranged by size:
1158 933 30
BsmI GAATG_Cn'
Cuts at: 0 591 1636 2121
Size: 591 1045 485
Fragments arranged by size:
1045 591 485
Bsp1286I G_dGCh'C
Cuts at: 0 1158 1188 2121
Size: 1158 30 933
Fragments arranged by size:
1158 933 30
BspEI T'CCGG_A
Cuts at: 0 6 1895 2121
Size: 6 1889 226
Fragments arranged by size:
1889 226 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1823 2121
Size: 1823 298
BsrDI GCAATG_nn'
Cuts at: 0 1031 1751 1790 2121
Size: 1031 720 39 331
Fragments arranged by size:
1031 720 331 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1567 2121
Size: 1567 554
BstYI r'GATC_y
Cuts at: 0 715 1211 1333 1605 1898 2121
Size: 715 496 122 272 293 223
Fragments arranged by size:
715 496 293 272 223 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1121 2121
Size: 1121 1000
BtsI GCAGTG_nn'
Cuts at: 0 137 2109 2121
Size: 137 1972 12
Fragments arranged by size:
1972 137 12
DraI TTT'AAA
Cuts at: 0 515 662 1520 2121
Size: 515 147 858 601
Fragments arranged by size:
858 601 515 147
EaeI y'GGCC_r
Cuts at: 0 1718 2121
Size: 1718 403
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 297 2121
Size: 297 1824
EcoICRI GAG'CTC
Cuts at: 0 1186 2121
Size: 1186 935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1008 1858 2121
Size: 446 562 850 263
Fragments arranged by size:
850 562 446 263
EcoO109I rG'GnC_Cy
Cuts at: 0 816 1869 2121
Size: 816 1053 252
Fragments arranged by size:
1053 816 252
EcoRV GAT'ATC
Cuts at: 0 1830 2121
Size: 1830 291
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 226 451 2121
Size: 226 225 1670
Fragments arranged by size:
1670 226 225
HaeII r_GCGC'y
Cuts at: 0 1509 2121
Size: 1509 612
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 586 618 1780 1812 2121
Size: 586 32 1162 32 309
Fragments arranged by size:
1162 586 309 32 32
HincII GTy'rAC
Cuts at: 0 2093 2121
Size: 2093 28
HindIII A'AGCT_T
Cuts at: 0 1106 2121
Size: 1106 1015
MfeI C'AATT_G
Cuts at: 0 346 2121
Size: 346 1775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 2121
Size: 723 1398
MscI TGG'CCA
Cuts at: 0 1720 2121
Size: 1720 401
MslI CAynn'nnrTG
Cuts at: 0 1294 1670 2121
Size: 1294 376 451
Fragments arranged by size:
1294 451 376
MspA1I CmG'CkG
Cuts at: 0 1043 1692 2121
Size: 1043 649 429
Fragments arranged by size:
1043 649 429
NsiI A_TGCA'T
Cuts at: 0 1061 1248 2121
Size: 1061 187 873
Fragments arranged by size:
1061 873 187
NspI r_CATG'y
Cuts at: 0 140 1080 2121
Size: 140 940 1041
Fragments arranged by size:
1041 940 140
PpuMI rG'GwC_Cy
Cuts at: 0 816 1869 2121
Size: 816 1053 252
Fragments arranged by size:
1053 816 252
PstI C_TGCA'G
Cuts at: 0 393 2121
Size: 393 1728
PvuII CAG'CTG
Cuts at: 0 1043 1692 2121
Size: 1043 649 429
Fragments arranged by size:
1043 649 429
SacI G_AGCT'C
Cuts at: 0 1188 2121
Size: 1188 933
SfcI C'TryA_G
Cuts at: 0 27 389 729 2121
Size: 27 362 340 1392
Fragments arranged by size:
1392 362 340 27
SmaI CCC'GGG
Cuts at: 0 812 2121
Size: 812 1309
SmlI C'TyrA_G
Cuts at: 0 1527 1864 1903 2121
Size: 1527 337 39 218
Fragments arranged by size:
1527 337 218 39
SspI AAT'ATT
Cuts at: 0 488 1926 2121
Size: 488 1438 195
Fragments arranged by size:
1438 488 195
StyI C'CwwG_G
Cuts at: 0 1017 1560 1592 2121
Size: 1017 543 32 529
Fragments arranged by size:
1017 543 529 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1018 2121
Size: 1018 1103
TatI w'GTAC_w
Cuts at: 0 1174 2121
Size: 1174 947
XmaI C'CCGG_G
Cuts at: 0 810 2121
Size: 810 1311
XmnI GAAnn'nnTTC
Cuts at: 0 420 431 2121
Size: 420 11 1690
Fragments arranged by size:
1690 420 11
Enzymes that do cut:
AccI AcuI AfeI AflII AlfI AlwNI ApaLI ApoI
AseI AvaI BamHI BanII BbsI BciVI BfrBI BglII
Bme1580I BpuEI BsaWI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrDI BstXI BstYI BtgZI BtsI DraI
EaeI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NsiI NspI PpuMI PstI PvuII SacI SfcI SmaI
SmlI SspI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflIII AgeI AhdI AleI
AloI ApaI AscI AsiSI AvrII BaeI BanI BbeI
BbvCI BcgI BclI BglI BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BsiEI BsiWI BsmBI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
ClaI CspCI DraIII DrdI EagI EarI EcoNI EcoRI
FseI FspI FspAI HpaI KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XbaI XcmI XhoI ZraI