Restriction map for
pFN21AE3918
(Linear) (Six-base) MAPSORT of: KIEE3918.seq Check: 5313 from: 1 to: 1821
KIEE3918.orf
With 182 enzymes: SgfI *
March 22, 2012 16:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1056 1821
Size: 1056 765
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1261 1821
Size: 1123 138 560
Fragments arranged by size:
1123 560 138
AfeI AGC'GCT
Cuts at: 0 1276 1821
Size: 1276 545
AflIII A'CryG_T
Cuts at: 0 770 1821
Size: 770 1051
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 424 1821
Size: 424 1397
AlwNI CAG_nnn'CTG
Cuts at: 0 339 732 1695 1821
Size: 339 393 963 126
Fragments arranged by size:
963 393 339 126
ApaLI G'TGCA_C
Cuts at: 0 313 1821
Size: 313 1508
AvaI C'yCGr_G
Cuts at: 0 639 683 863 1432 1747 1821
Size: 639 44 180 569 315 74
Fragments arranged by size:
639 569 315 180 74 44
AvrII C'CTAG_G
Cuts at: 0 75 1821
Size: 75 1746
BanI G'GyrC_C
Cuts at: 0 49 291 643 1328 1402 1821
Size: 49 242 352 685 74 419
Fragments arranged by size:
685 419 352 242 74 49
BanII G_rGCy'C
Cuts at: 0 38 119 920 986 1124 1253 1507 1634
Size: 38 81 801 66 138 129 254 127
Cuts at: 1634 1821
Size: 187
Fragments arranged by size:
801 254 187 138 129 127 81 66
38
BbeI G_GCGC'C
Cuts at: 0 1332 1406 1821
Size: 1332 74 415
Fragments arranged by size:
1332 415 74
BbvCI CC'TCA_GC
Cuts at: 0 94 1821
Size: 94 1727
Bme1580I G_kGCm'C
Cuts at: 0 296 317 684 746 856 1204 1464 1742
Size: 296 21 367 62 110 348 260 278
Cuts at: 1742 1769 1821
Size: 27 52
Fragments arranged by size:
367 348 296 278 260 110 62 52
27 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 709 1821
Size: 709 1112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 939 1521 1821
Size: 939 582 300
Fragments arranged by size:
939 582 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 259 559 754 768 1138 1243 1441
Size: 22 237 300 195 14 370 105 198
Cuts at: 1441 1705 1744 1750 1821
Size: 264 39 6 71
Fragments arranged by size:
370 300 264 237 198 195 105 71
39 22 14 6
Bpu10I CC'TnA_GC
Cuts at: 0 94 1821
Size: 94 1727
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 1821
Size: 273 1548
BsaI GGTCTCn'nnnn_
Cuts at: 0 1699 1821
Size: 1699 122
BsaAI yAC'GTr
Cuts at: 0 186 510 1821
Size: 186 324 1311
Fragments arranged by size:
1311 324 186
BsaHI Gr'CG_yC
Cuts at: 0 556 1329 1403 1821
Size: 556 773 74 418
Fragments arranged by size:
773 556 418 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 619 649 1194 1224 1561 1591 1821
Size: 619 30 545 30 337 30 230
Fragments arranged by size:
619 545 337 230 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1411 1582 1821
Size: 1411 171 239
Fragments arranged by size:
1411 239 171
BseYI C'CCAG_C
Cuts at: 0 261 528 720 796 1644 1821
Size: 261 267 192 76 848 177
Fragments arranged by size:
848 267 261 192 177 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 1174 1821
Size: 491 683 647
Fragments arranged by size:
683 647 491
BsiHKAI G_wGCw'C
Cuts at: 0 119 128 317 986 1124 1253 1821
Size: 119 9 189 669 138 129 568
Fragments arranged by size:
669 568 189 138 129 119 9
BsmI GAATG_Cn'
Cuts at: 0 1146 1821
Size: 1146 675
Bsp1286I G_dGCh'C
Cuts at: 0 38 119 128 296 317 684 746 856
Size: 38 81 9 168 21 367 62 110
Cuts at: 856 920 986 1124 1204 1253 1464 1507 1634
Size: 64 66 138 80 49 211 43 127
Cuts at: 1634 1742 1769 1821
Size: 108 27 52
Fragments arranged by size:
367 211 168 138 127 110 108 81
80 66 64 62 52 49 43 38
27 21 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 588 1056 1821
Size: 588 468 765
Fragments arranged by size:
765 588 468
BsrBI CCG'CTC
Cuts at: 0 1752 1821
Size: 1752 69
BsrDI GCAATG_nn'
Cuts at: 0 1589 1821
Size: 1589 232
BsrFI r'CCGG_y
Cuts at: 0 809 1322 1821
Size: 809 513 499
Fragments arranged by size:
809 513 499
BssSI C'ACGA_G
Cuts at: 0 847 979 1821
Size: 847 132 842
Fragments arranged by size:
847 842 132
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1071 1278 1481 1821
Size: 1071 207 203 340
Fragments arranged by size:
1071 340 207 203
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 393 1116 1821
Size: 354 39 723 705
Fragments arranged by size:
723 705 354 39
Bsu36I CC'TnA_GG
Cuts at: 0 100 1821
Size: 100 1721
BtgI C'CryG_G
Cuts at: 0 353 1560 1655 1700 1821
Size: 353 1207 95 45 121
Fragments arranged by size:
1207 353 121 95 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1794 1821
Size: 1794 27
DraIII CAC_nnn'GTG
Cuts at: 0 132 1821
Size: 132 1689
EaeI y'GGCC_r
Cuts at: 0 960 1448 1753 1821
Size: 960 488 305 68
Fragments arranged by size:
960 488 305 68
EarI CTCTTCn'nnn_
Cuts at: 0 991 1129 1309 1821
Size: 991 138 180 512
Fragments arranged by size:
991 512 180 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1255 1273 1821
Size: 1255 18 548
Fragments arranged by size:
1255 548 18
EcoICRI GAG'CTC
Cuts at: 0 117 984 1122 1251 1821
Size: 117 867 138 129 570
Fragments arranged by size:
867 570 138 129 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 259 559 754 768 1123 1138 1243
Size: 22 237 300 195 14 355 15 105
Cuts at: 1243 1261 1441 1705 1744 1750 1821
Size: 18 180 264 39 6 71
Fragments arranged by size:
355 300 264 237 195 180 105 71
39 22 18 15 14 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 165 607 1291 1821
Size: 79 86 442 684 530
Fragments arranged by size:
684 530 442 86 79
EcoO109I rG'GnC_Cy
Cuts at: 0 104 600 729 1524 1821
Size: 104 496 129 795 297
Fragments arranged by size:
795 496 297 129 104
HaeII r_GCGC'y
Cuts at: 0 1278 1332 1406 1821
Size: 1278 54 74 415
Fragments arranged by size:
1278 415 74 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 483 515 619 651 1821
Size: 483 32 104 32 1170
Fragments arranged by size:
1170 483 104 32 32
KasI G'GCGC_C
Cuts at: 0 1328 1402 1821
Size: 1328 74 419
Fragments arranged by size:
1328 419 74
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 800 1821
Size: 800 1021
MslI CAynn'nnrTG
Cuts at: 0 1066 1821
Size: 1066 755
MspA1I CmG'CkG
Cuts at: 0 276 339 585 594 879 1005 1083 1692
Size: 276 63 246 9 285 126 78 609
Cuts at: 1692 1821
Size: 129
Fragments arranged by size:
609 285 276 246 129 126 78 63
9
NaeI GCC'GGC
Cuts at: 0 1324 1821
Size: 1324 497
NarI GG'CG_CC
Cuts at: 0 1329 1403 1821
Size: 1329 74 418
Fragments arranged by size:
1329 418 74
NcoI C'CATG_G
Cuts at: 0 353 1655 1700 1821
Size: 353 1302 45 121
Fragments arranged by size:
1302 353 121 45
NgoMIV G'CCGG_C
Cuts at: 0 1322 1821
Size: 1322 499
NspI r_CATG'y
Cuts at: 0 411 552 774 1821
Size: 411 141 222 1047
Fragments arranged by size:
1047 411 222 141
PasI CC'CwG_GG
Cuts at: 0 267 1821
Size: 267 1554
PciI A'CATG_T
Cuts at: 0 770 1821
Size: 770 1051
PfoI T'CCnGG_A
Cuts at: 0 1493 1507 1821
Size: 1493 14 314
Fragments arranged by size:
1493 314 14
PpuMI rG'GwC_Cy
Cuts at: 0 600 729 1524 1821
Size: 600 129 795 297
Fragments arranged by size:
795 600 297 129
PstI C_TGCA'G
Cuts at: 0 380 518 599 1382 1807 1821
Size: 380 138 81 783 425 14
Fragments arranged by size:
783 425 380 138 81 14
PvuII CAG'CTG
Cuts at: 0 276 339 594 879 1005 1083 1692 1821
Size: 276 63 255 285 126 78 609 129
Fragments arranged by size:
609 285 276 255 129 126 78 63
SacI G_AGCT'C
Cuts at: 0 119 986 1124 1253 1821
Size: 119 867 138 129 568
Fragments arranged by size:
867 568 138 129 119
SapI GCTCTTCn'nnn_
Cuts at: 0 991 1129 1309 1821
Size: 991 138 180 512
Fragments arranged by size:
991 512 180 138
SfcI C'TryA_G
Cuts at: 0 319 376 514 570 595 1378 1803 1821
Size: 319 57 138 56 25 783 425 18
Fragments arranged by size:
783 425 319 138 57 56 25 18
SfoI GGC'GCC
Cuts at: 0 1330 1404 1821
Size: 1330 74 417
Fragments arranged by size:
1330 417 74
SmaI CCC'GGG
Cuts at: 0 865 1821
Size: 865 956
SmlI C'TyrA_G
Cuts at: 0 252 1821
Size: 252 1569
SnaBI TAC'GTA
Cuts at: 0 510 1821
Size: 510 1311
SphI G_CATG'C
Cuts at: 0 552 1821
Size: 552 1269
StyI C'CwwG_G
Cuts at: 0 75 228 347 353 1133 1655 1700 1821
Size: 75 153 119 6 780 522 45 121
Fragments arranged by size:
780 522 153 121 119 75 45 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 963 1821
Size: 963 858
TatI w'GTAC_w
Cuts at: 0 776 1821
Size: 776 1045
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1194 1226 1327 1359 1821
Size: 1194 32 101 32 462
Fragments arranged by size:
1194 462 101 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1057 1821
Size: 1057 764
XmaI C'CCGG_G
Cuts at: 0 863 1821
Size: 863 958
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AlwNI ApaLI AvaI
AvrII BanI BanII BbeI BbvCI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI Bsu36I BtgI BtgZI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PpuMI PstI PvuII
SacI SapI SfcI SfoI SmaI SmlI SnaBI SphI
StyI TaqII TatI TstI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AloI ApaI ApoI AscI AseI AsiSI BaeI BamHI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BsrGI BssHII BstBI BstEII BstYI BstZ17I BtsI
ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NdeI NheI NotI NruI NsiI PacI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SpeI SrfI SspI StuI
SwaI XbaI XcmI XhoI XmnI ZraI