Restriction map for
pFN21AE3917
(Linear) (Six-base) MAPSORT of: KIEE3917.seq Check: 8915 from: 1 to: 834
KIEE3917.orf
With 182 enzymes: SgfI *
March 22, 2012 16:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 72 834
Size: 72 762
AccI GT'mk_AC
Cuts at: 0 587 834
Size: 587 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 649 834
Size: 339 310 185
Fragments arranged by size:
339 310 185
AgeI A'CCGG_T
Cuts at: 0 414 834
Size: 414 420
AleI CACnn'nnGTG
Cuts at: 0 806 834
Size: 806 28
AlwNI CAG_nnn'CTG
Cuts at: 0 87 362 516 834
Size: 87 275 154 318
Fragments arranged by size:
318 275 154 87
ApaI G_GGCC'C
Cuts at: 0 167 834
Size: 167 667
AvaI C'yCGr_G
Cuts at: 0 566 834
Size: 566 268
BamHI G'GATC_C
Cuts at: 0 821 834
Size: 821 13
BanI G'GyrC_C
Cuts at: 0 4 417 787 834
Size: 4 413 370 47
Fragments arranged by size:
413 370 47 4
BanII G_rGCy'C
Cuts at: 0 167 429 834
Size: 167 262 405
Fragments arranged by size:
405 262 167
BbeI G_GCGC'C
Cuts at: 0 8 791 834
Size: 8 783 43
Fragments arranged by size:
783 43 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 263 696 834
Size: 263 433 138
Fragments arranged by size:
433 263 138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 557 591 834
Size: 557 34 243
Fragments arranged by size:
557 243 34
BglII A'GATC_T
Cuts at: 0 539 834
Size: 539 295
BlpI GC'TnA_GC
Cuts at: 0 88 834
Size: 88 746
Bme1580I G_kGCm'C
Cuts at: 0 167 782 834
Size: 167 615 52
Fragments arranged by size:
615 167 52
BmgBI CAC'GTC
Cuts at: 0 702 762 834
Size: 702 60 72
Fragments arranged by size:
702 72 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 124 834
Size: 124 710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 789 834
Size: 789 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 699 834
Size: 699 135
BsaAI yAC'GTr
Cuts at: 0 96 735 834
Size: 96 639 99
Fragments arranged by size:
639 99 96
BsaBI GATnn'nnATC
Cuts at: 0 538 834
Size: 538 296
BsaHI Gr'CG_yC
Cuts at: 0 5 788 834
Size: 5 783 46
Fragments arranged by size:
783 46 5
BsaWI w'CCGG_w
Cuts at: 0 414 834
Size: 414 420
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 834
Size: 280 554
BseYI C'CCAG_C
Cuts at: 0 247 362 389 834
Size: 247 115 27 445
Fragments arranged by size:
445 247 115 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 834
Size: 100 734
BsiEI CG_ry'CG
Cuts at: 0 198 318 834
Size: 198 120 516
Fragments arranged by size:
516 198 120
BsiHKAI G_wGCw'C
Cuts at: 0 469 834
Size: 469 365
BsmBI CGTCTCn'nnnn_
Cuts at: 0 752 834
Size: 752 82
Bsp1286I G_dGCh'C
Cuts at: 0 167 429 469 782 834
Size: 167 262 40 313 52
Fragments arranged by size:
313 262 167 52 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 72 448 834
Size: 72 376 386
Fragments arranged by size:
386 376 72
BsrFI r'CCGG_y
Cuts at: 0 30 414 834
Size: 30 384 420
Fragments arranged by size:
420 384 30
BssHII G'CGCG_C
Cuts at: 0 289 303 305 834
Size: 289 14 2 529
Fragments arranged by size:
529 289 14 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 516 834
Size: 516 318
BstBI TT'CG_AA
Cuts at: 0 458 834
Size: 458 376
BstYI r'GATC_y
Cuts at: 0 539 821 834
Size: 539 282 13
Fragments arranged by size:
539 282 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 135 834
Size: 135 699
BtsI GCAGTG_nn'
Cuts at: 0 233 834
Size: 233 601
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 92 127 834
Size: 92 35 707
Fragments arranged by size:
707 92 35
DraIII CAC_nnn'GTG
Cuts at: 0 173 834
Size: 173 661
EaeI y'GGCC_r
Cuts at: 0 315 834
Size: 315 519
EagI C'GGCC_G
Cuts at: 0 315 834
Size: 315 519
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 592 834
Size: 49 543 242
Fragments arranged by size:
543 242 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 649 789 834
Size: 339 310 140 45
Fragments arranged by size:
339 310 140 45
HaeII r_GCGC'y
Cuts at: 0 8 206 530 566 791 834
Size: 8 198 324 36 225 43
Fragments arranged by size:
324 225 198 43 36 8
KasI G'GCGC_C
Cuts at: 0 4 787 834
Size: 4 783 47
Fragments arranged by size:
783 47 4
MslI CAynn'nnrTG
Cuts at: 0 806 834
Size: 806 28
MspA1I CmG'CkG
Cuts at: 0 186 276 834
Size: 186 90 558
Fragments arranged by size:
558 186 90
NaeI GCC'GGC
Cuts at: 0 32 834
Size: 32 802
NarI GG'CG_CC
Cuts at: 0 5 788 834
Size: 5 783 46
Fragments arranged by size:
783 46 5
NgoMIV G'CCGG_C
Cuts at: 0 30 834
Size: 30 804
PflMI CCAn_nnn'nTGG
Cuts at: 0 173 834
Size: 173 661
PmlI CAC'GTG
Cuts at: 0 96 834
Size: 96 738
PspOMI G'GGCC_C
Cuts at: 0 163 834
Size: 163 671
PstI C_TGCA'G
Cuts at: 0 185 834
Size: 185 649
PvuII CAG'CTG
Cuts at: 0 186 834
Size: 186 648
SexAI A'CCwGG_T
Cuts at: 0 488 834
Size: 488 346
SfcI C'TryA_G
Cuts at: 0 181 834
Size: 181 653
SfoI GGC'GCC
Cuts at: 0 6 789 834
Size: 6 783 45
Fragments arranged by size:
783 45 6
SgrAI Cr'CCGG_yG
Cuts at: 0 414 834
Size: 414 420
SmlI C'TyrA_G
Cuts at: 0 566 834
Size: 566 268
SnaBI TAC'GTA
Cuts at: 0 735 834
Size: 735 99
StyI C'CwwG_G
Cuts at: 0 110 834
Size: 110 724
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 762 834
Size: 762 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 490 522 834
Size: 490 32 312
Fragments arranged by size:
490 312 32
Tth111I GACn'n_nGTC
Cuts at: 0 658 834
Size: 658 176
XhoI C'TCGA_G
Cuts at: 0 566 834
Size: 566 268
Enzymes that do cut:
AarI AccI AcuI AgeI AleI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BcgI BglII BlpI
Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrFI BssHII BstAPI BstBI BstYI BtgZI BtsI
CspCI DraIII EaeI EagI EciI Eco57MI HaeII KasI
MslI MspA1I NaeI NarI NgoMIV PflMI PmlI PspOMI
PstI PvuII SexAI SfcI SfoI SgrAI SmlI SnaBI
StyI TaqII TstI Tth111I XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BclI BfrBI BglI BmtI BplI Bpu10I
BpuEI BsaXI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrGI BssSI BstEII BstXI BstZ17I Bsu36I BtgI ClaI
DraI DrdI EarI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SgfI SmaI SpeI SphI SrfI SspI StuI
SwaI TatI XbaI XcmI XmaI XmnI ZraI