(Linear) (Six-base) MAPSORT of: KIEE3915.seq  Check: 4344  from: 1  to: 345



KIEE3915.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     345 
   Size:        292      53
 
AvrII C'CTAG_G

Cuts at:      0     138     345 
   Size:        138     207
 
BanI G'GyrC_C

Cuts at:      0     171     345 
   Size:        171     174
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      86     345 
   Size:         86     259
 
Bme1580I G_kGCm'C

Cuts at:      0     176     345 
   Size:        176     169
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     167     345 
   Size:        167     178
 
BsaAI yAC'GTr

Cuts at:      0     239     345 
   Size:        239     106
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     240     270     345 
   Size:        240      30      75

  Fragments arranged by size: 

                 240      75      30
 
BseYI C'CCAG_C

Cuts at:      0      55     216     345 
   Size:         55     161     129

  Fragments arranged by size: 

                 161     129      55
 
BsiWI C'GTAC_G

Cuts at:      0     239     345 
   Size:        239     106
 
BsmI GAATG_Cn'

Cuts at:      0     339     345 
   Size:        339       6
 
Bsp1286I G_dGCh'C

Cuts at:      0     176     345 
   Size:        176     169
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      84     157     345 
   Size:         84      73     188

  Fragments arranged by size: 

                 188      84      73
 
BstYI r'GATC_y

Cuts at:      0     340     345 
   Size:        340       5
 
BtgI C'CryG_G

Cuts at:      0     260     345 
   Size:        260      85
 
EaeI y'GGCC_r

Cuts at:      0      20     200     345 
   Size:         20     180     145

  Fragments arranged by size: 

                 180     145      20
 
EarI CTCTTCn'nnn_

Cuts at:      0     206     345 
   Size:        206     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     345 
   Size:        292      53
 
MscI TGG'CCA

Cuts at:      0     202     345 
   Size:        202     143
 
MslI CAynn'nnrTG

Cuts at:      0      82     345 
   Size:         82     263
 
MspA1I CmG'CkG

Cuts at:      0      55     111     345 
   Size:         55      56     234

  Fragments arranged by size: 

                 234      56      55
 
NcoI C'CATG_G

Cuts at:      0     260     345 
   Size:        260      85
 
NspI r_CATG'y

Cuts at:      0      33     345 
   Size:         33     312
 
PasI CC'CwG_GG

Cuts at:      0     150     345 
   Size:        150     195
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     287     319     345 
   Size:        287      32      26

  Fragments arranged by size: 

                 287      32      26
 
PvuII CAG'CTG

Cuts at:      0     111     345 
   Size:        111     234
 
SapI GCTCTTCn'nnn_

Cuts at:      0     206     345 
   Size:        206     139
 
StyI C'CwwG_G

Cuts at:      0     138     260     345 
   Size:        138     122      85

  Fragments arranged by size: 

                 138     122      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     163     345 
   Size:        163     182
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      58      90     240     272     345 
   Size:         58      32     150      32      73

  Fragments arranged by size: 

                 150      73      58      32      32
 

 Enzymes that do cut:

     AcuI    AvrII     BanI     BbsI Bme1580I     BmrI    BsaAI    BsaXI
    BseYI    BsiWI     BsmI Bsp1286I    BstXI    BstYI     BtgI     EaeI
     EarI  Eco57MI     MscI     MslI   MspA1I     NcoI     NspI     PasI
     PpiI    PvuII     SapI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI