Restriction map for
pFN21AE3913
(Linear) (Six-base) MAPSORT of: KIEE3913.seq Check: 4445 from: 1 to: 630
KIEE3913.orf
With 182 enzymes: SgfI *
March 22, 2012 16:49 ..
AatII G_ACGT'C
Cuts at: 0 94 630
Size: 94 536
Acc65I G'GTAC_C
Cuts at: 0 332 363 630
Size: 332 31 267
Fragments arranged by size:
332 267 31
AgeI A'CCGG_T
Cuts at: 0 326 630
Size: 326 304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 630
Size: 208 422
ApaLI G'TGCA_C
Cuts at: 0 193 630
Size: 193 437
AvaI C'yCGr_G
Cuts at: 0 127 505 630
Size: 127 378 125
Fragments arranged by size:
378 127 125
BanI G'GyrC_C
Cuts at: 0 30 332 363 630
Size: 30 302 31 267
Fragments arranged by size:
302 267 31 30
BbeI G_GCGC'C
Cuts at: 0 34 630
Size: 34 596
BbsI GAAGACnn'nnnn_
Cuts at: 0 94 99 537 630
Size: 94 5 438 93
Fragments arranged by size:
438 94 93 5
BbvCI CC'TCA_GC
Cuts at: 0 571 630
Size: 571 59
BglI GCCn_nnn'nGGC
Cuts at: 0 118 565 630
Size: 118 447 65
Fragments arranged by size:
447 118 65
Bme1580I G_kGCm'C
Cuts at: 0 197 630
Size: 197 433
Bpu10I CC'TnA_GC
Cuts at: 0 571 630
Size: 571 59
BsaHI Gr'CG_yC
Cuts at: 0 31 91 263 630
Size: 31 60 172 367
Fragments arranged by size:
367 172 60 31
BsaWI w'CCGG_w
Cuts at: 0 326 630
Size: 326 304
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 630
Size: 40 590
BsiEI CG_ry'CG
Cuts at: 0 585 630
Size: 585 45
BsiHKAI G_wGCw'C
Cuts at: 0 197 514 630
Size: 197 317 116
Fragments arranged by size:
317 197 116
Bsp1286I G_dGCh'C
Cuts at: 0 197 514 630
Size: 197 317 116
Fragments arranged by size:
317 197 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 404 630
Size: 179 225 226
Fragments arranged by size:
226 225 179
BsrBI CCG'CTC
Cuts at: 0 156 630
Size: 156 474
BsrFI r'CCGG_y
Cuts at: 0 326 630
Size: 326 304
BstEII G'GTnAC_C
Cuts at: 0 624 630
Size: 624 6
BtgI C'CryG_G
Cuts at: 0 17 143 485 579 630
Size: 17 126 342 94 51
Fragments arranged by size:
342 126 94 51 17
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 132 167 630
Size: 132 35 463
Fragments arranged by size:
463 132 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 268 630
Size: 268 362
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 615 630
Size: 615 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 400 459 630
Size: 400 59 171
Fragments arranged by size:
400 171 59
EcoO109I rG'GnC_Cy
Cuts at: 0 119 630
Size: 119 511
FspI TGC'GCA
Cuts at: 0 400 630
Size: 400 230
HaeII r_GCGC'y
Cuts at: 0 34 630
Size: 34 596
HincII GTy'rAC
Cuts at: 0 168 630
Size: 168 462
KasI G'GCGC_C
Cuts at: 0 30 630
Size: 30 600
KpnI G_GTAC'C
Cuts at: 0 336 367 630
Size: 336 31 263
Fragments arranged by size:
336 263 31
MslI CAynn'nnrTG
Cuts at: 0 376 630
Size: 376 254
MspA1I CmG'CkG
Cuts at: 0 19 581 630
Size: 19 562 49
Fragments arranged by size:
562 49 19
NarI GG'CG_CC
Cuts at: 0 31 630
Size: 31 599
PasI CC'CwG_GG
Cuts at: 0 609 630
Size: 609 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 474 630
Size: 474 156
PfoI T'CCnGG_A
Cuts at: 0 548 630
Size: 548 82
PspXI vC'TCGA_Gb
Cuts at: 0 505 630
Size: 505 125
RsrII CG'GwC_CG
Cuts at: 0 47 630
Size: 47 583
SacII CC_GC'GG
Cuts at: 0 20 582 630
Size: 20 562 48
Fragments arranged by size:
562 48 20
SfoI GGC'GCC
Cuts at: 0 32 630
Size: 32 598
SmlI C'TyrA_G
Cuts at: 0 505 630
Size: 505 125
Tth111I GACn'n_nGTC
Cuts at: 0 532 630
Size: 532 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 630
Size: 375 255
XhoI C'TCGA_G
Cuts at: 0 505 630
Size: 505 125
XmnI GAAnn'nnTTC
Cuts at: 0 349 630
Size: 349 281
ZraI GAC'GTC
Cuts at: 0 92 630
Size: 92 538
Enzymes that do cut:
AatII Acc65I AgeI AlfI ApaLI AvaI BanI BbeI
BbsI BbvCI BglI Bme1580I Bpu10I BsaHI BsaWI BseRI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstEII BtgI
CspCI DrdI EciI EcoNI EcoO109I FspI HaeII HincII
KasI KpnI MslI MspA1I NarI PasI PflMI PfoI
PspXI RsrII SacII SfoI SmlI Tth111I XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AflIII AhdI
AleI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaXI BseYI BsgI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
DraI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoRI
EcoRV FalI FseI FspAI Hin4I HindIII HpaI MfeI
MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
XbaI XmaI