Restriction map for
pFN21AE3912
(Linear) (Six-base) MAPSORT of: KIEE3912.seq Check: 5825 from: 1 to: 1248
KIEE3912.orf
With 182 enzymes: SgfI *
March 22, 2012 16:49 ..
AccI GT'mk_AC
Cuts at: 0 557 1187 1248
Size: 557 630 61
Fragments arranged by size:
630 557 61
AflIII A'CryG_T
Cuts at: 0 791 1248
Size: 791 457
AlwNI CAG_nnn'CTG
Cuts at: 0 507 526 719 1069 1248
Size: 507 19 193 350 179
Fragments arranged by size:
507 350 193 179 19
AvaI C'yCGr_G
Cuts at: 0 418 1201 1248
Size: 418 783 47
Fragments arranged by size:
783 418 47
BanI G'GyrC_C
Cuts at: 0 199 1248
Size: 199 1049
BanII G_rGCy'C
Cuts at: 0 1202 1248
Size: 1202 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 367 1248
Size: 367 881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 244 278 1248
Size: 244 34 970
Fragments arranged by size:
970 244 34
BfrBI ATG'CAT
Cuts at: 0 36 1248
Size: 36 1212
BglI GCCn_nnn'nGGC
Cuts at: 0 691 1248
Size: 691 557
BglII A'GATC_T
Cuts at: 0 641 1248
Size: 641 607
Bme1580I G_kGCm'C
Cuts at: 0 202 221 514 1248
Size: 202 19 293 734
Fragments arranged by size:
734 293 202 19
BmgBI CAC'GTC
Cuts at: 0 471 1248
Size: 471 777
BmtI G_CTAG'C
Cuts at: 0 1063 1248
Size: 1063 185
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 647 762 1248
Size: 647 115 486
Fragments arranged by size:
647 486 115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 757 1247 1248
Size: 206 551 490 1
Fragments arranged by size:
551 490 206 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1248
Size: 1082 166
BsaI GGTCTCn'nnnn_
Cuts at: 0 323 1248
Size: 323 925
BsaAI yAC'GTr
Cuts at: 0 794 1248
Size: 794 454
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 523 553 1248
Size: 179 30 314 30 695
Fragments arranged by size:
695 314 179 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 774 1248
Size: 774 474
BseYI C'CCAG_C
Cuts at: 0 225 365 407 412 526 1248
Size: 225 140 42 5 114 722
Fragments arranged by size:
722 225 140 114 42 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 547 732 1248
Size: 171 376 185 516
Fragments arranged by size:
516 376 185 171
BsiHKAI G_wGCw'C
Cuts at: 0 7 1202 1248
Size: 7 1195 46
Fragments arranged by size:
1195 46 7
BsmI GAATG_Cn'
Cuts at: 0 432 874 1248
Size: 432 442 374
Fragments arranged by size:
442 432 374
Bsp1286I G_dGCh'C
Cuts at: 0 7 202 221 514 1202 1248
Size: 7 195 19 293 688 46
Fragments arranged by size:
688 293 195 46 19 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 14 569 1015 1248
Size: 14 555 446 233
Fragments arranged by size:
555 446 233 14
BsrFI r'CCGG_y
Cuts at: 0 536 1160 1248
Size: 536 624 88
Fragments arranged by size:
624 536 88
BssSI C'ACGA_G
Cuts at: 0 165 664 1248
Size: 165 499 584
Fragments arranged by size:
584 499 165
BstAPI GCAn_nnn'nTGC
Cuts at: 0 600 719 1248
Size: 600 119 529
Fragments arranged by size:
600 529 119
BstBI TT'CG_AA
Cuts at: 0 659 1248
Size: 659 589
BstYI r'GATC_y
Cuts at: 0 641 1248
Size: 641 607
BtgI C'CryG_G
Cuts at: 0 203 1248
Size: 203 1045
BtsI GCAGTG_nn'
Cuts at: 0 260 823 1248
Size: 260 563 425
Fragments arranged by size:
563 425 260
DraIII CAC_nnn'GTG
Cuts at: 0 216 1248
Size: 216 1032
EarI CTCTTCn'nnn_
Cuts at: 0 1216 1248
Size: 1216 32
EcoICRI GAG'CTC
Cuts at: 0 1200 1248
Size: 1200 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 757 1247 1248
Size: 206 551 490 1
Fragments arranged by size:
551 490 206 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 739 1248
Size: 739 509
EcoO109I rG'GnC_Cy
Cuts at: 0 170 1004 1206 1248
Size: 170 834 202 42
Fragments arranged by size:
834 202 170 42
EcoRV GAT'ATC
Cuts at: 0 881 1248
Size: 881 367
HincII GTy'rAC
Cuts at: 0 653 1188 1248
Size: 653 535 60
Fragments arranged by size:
653 535 60
HindIII A'AGCT_T
Cuts at: 0 947 1248
Size: 947 301
MfeI C'AATT_G
Cuts at: 0 780 1248
Size: 780 468
MslI CAynn'nnrTG
Cuts at: 0 727 1248
Size: 727 521
MspA1I CmG'CkG
Cuts at: 0 184 395 507 523 1248
Size: 184 211 112 16 725
Fragments arranged by size:
725 211 184 112 16
NaeI GCC'GGC
Cuts at: 0 1162 1248
Size: 1162 86
NcoI C'CATG_G
Cuts at: 0 203 1248
Size: 203 1045
NgoMIV G'CCGG_C
Cuts at: 0 1160 1248
Size: 1160 88
NheI G'CTAG_C
Cuts at: 0 1059 1248
Size: 1059 189
NsiI A_TGCA'T
Cuts at: 0 38 1248
Size: 38 1210
NspI r_CATG'y
Cuts at: 0 651 1168 1248
Size: 651 517 80
Fragments arranged by size:
651 517 80
PfoI T'CCnGG_A
Cuts at: 0 734 1248
Size: 734 514
PpuMI rG'GwC_Cy
Cuts at: 0 1004 1206 1248
Size: 1004 202 42
Fragments arranged by size:
1004 202 42
PstI C_TGCA'G
Cuts at: 0 183 400 1248
Size: 183 217 848
Fragments arranged by size:
848 217 183
PvuII CAG'CTG
Cuts at: 0 184 395 507 523 1248
Size: 184 211 112 16 725
Fragments arranged by size:
725 211 184 112 16
SacI G_AGCT'C
Cuts at: 0 1202 1248
Size: 1202 46
SalI G'TCGA_C
Cuts at: 0 1186 1248
Size: 1186 62
ScaI AGT'ACT
Cuts at: 0 115 1248
Size: 115 1133
SfcI C'TryA_G
Cuts at: 0 179 396 489 585 1248
Size: 179 217 93 96 663
Fragments arranged by size:
663 217 179 96 93
SmlI C'TyrA_G
Cuts at: 0 1097 1248
Size: 1097 151
StuI AGG'CCT
Cuts at: 0 679 745 1248
Size: 679 66 503
Fragments arranged by size:
679 503 66
StyI C'CwwG_G
Cuts at: 0 203 1248
Size: 203 1045
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 347 620 900 1248
Size: 347 273 280 348
Fragments arranged by size:
348 347 280 273
TatI w'GTAC_w
Cuts at: 0 113 1248
Size: 113 1135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 235 267 694 726 1248
Size: 179 32 24 32 427 32 522
Fragments arranged by size:
522 427 179 32 32 32 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 905 1248
Size: 905 343
Enzymes that do cut:
AccI AflIII AlwNI AvaI BanI BanII BbsI BcgI
BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtsI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV HincII HindIII MfeI MslI MspA1I NaeI NcoI
NgoMIV NheI NsiI NspI PfoI PpuMI PstI PvuII
SacI SalI ScaI SfcI SmlI StuI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI
BclI BlpI BmrI Bpu10I BsaBI BsaHI BsaWI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EaeI EagI EciI EcoRI FalI FseI FspI
FspAI HaeII Hin4I HpaI KasI KpnI MluI MmeI
MscI NarI NdeI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI