Restriction map for
pFN21AE3909
(Linear) (Six-base) MAPSORT of: KIEE3909.seq Check: 4664 from: 1 to: 888
KIEE3909.orf
With 182 enzymes: SgfI *
March 22, 2012 16:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 277 888
Size: 277 611
AccI GT'mk_AC
Cuts at: 0 467 888
Size: 467 421
AfeI AGC'GCT
Cuts at: 0 61 888
Size: 61 827
AlwNI CAG_nnn'CTG
Cuts at: 0 314 888
Size: 314 574
ApaI G_GGCC'C
Cuts at: 0 534 888
Size: 534 354
BanI G'GyrC_C
Cuts at: 0 590 888
Size: 590 298
BanII G_rGCy'C
Cuts at: 0 110 534 888
Size: 110 424 354
Fragments arranged by size:
424 354 110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 426 460 633 643 667 677 888
Size: 426 34 173 10 24 10 211
Fragments arranged by size:
426 211 173 34 24 10 10
Bme1580I G_kGCm'C
Cuts at: 0 34 534 888
Size: 34 500 354
Fragments arranged by size:
500 354 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 253 888
Size: 253 635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 888
Size: 84 804
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 888
Size: 838 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 649 888
Size: 649 239
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 609 639 888
Size: 609 30 249
Fragments arranged by size:
609 249 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 192 888
Size: 192 696
BseYI C'CCAG_C
Cuts at: 0 72 324 888
Size: 72 252 564
Fragments arranged by size:
564 252 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 888
Size: 647 241
BsiHKAI G_wGCw'C
Cuts at: 0 239 888
Size: 239 649
Bsp1286I G_dGCh'C
Cuts at: 0 34 110 239 534 888
Size: 34 76 129 295 354
Fragments arranged by size:
354 295 129 76 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 277 432 888
Size: 277 155 456
Fragments arranged by size:
456 277 155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 561 888
Size: 561 327
Bsu36I CC'TnA_GG
Cuts at: 0 149 888
Size: 149 739
BtsI GCAGTG_nn'
Cuts at: 0 156 432 635 888
Size: 156 276 203 253
Fragments arranged by size:
276 253 203 156
DraIII CAC_nnn'GTG
Cuts at: 0 681 888
Size: 681 207
EaeI y'GGCC_r
Cuts at: 0 272 821 888
Size: 272 549 67
Fragments arranged by size:
549 272 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 888
Size: 84 804
EcoO109I rG'GnC_Cy
Cuts at: 0 530 888
Size: 530 358
HaeII r_GCGC'y
Cuts at: 0 63 804 888
Size: 63 741 84
Fragments arranged by size:
741 84 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 888
Size: 487 32 369
Fragments arranged by size:
487 369 32
MscI TGG'CCA
Cuts at: 0 274 823 888
Size: 274 549 65
Fragments arranged by size:
549 274 65
MspA1I CmG'CkG
Cuts at: 0 76 667 888
Size: 76 591 221
Fragments arranged by size:
591 221 76
NdeI CA'TA_TG
Cuts at: 0 339 888
Size: 339 549
NspI r_CATG'y
Cuts at: 0 45 441 786 888
Size: 45 396 345 102
Fragments arranged by size:
396 345 102 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 79 888
Size: 79 809
PfoI T'CCnGG_A
Cuts at: 0 386 888
Size: 386 502
PspOMI G'GGCC_C
Cuts at: 0 530 888
Size: 530 358
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 208 240 888
Size: 208 32 648
Fragments arranged by size:
648 208 32
PstI C_TGCA'G
Cuts at: 0 666 888
Size: 666 222
PvuII CAG'CTG
Cuts at: 0 76 667 888
Size: 76 591 221
Fragments arranged by size:
591 221 76
SfcI C'TryA_G
Cuts at: 0 662 888
Size: 662 226
SmlI C'TyrA_G
Cuts at: 0 853 888
Size: 853 35
SphI G_CATG'C
Cuts at: 0 45 441 786 888
Size: 45 396 345 102
Fragments arranged by size:
396 345 102 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 497 888
Size: 497 391
TatI w'GTAC_w
Cuts at: 0 116 882 888
Size: 116 766 6
Fragments arranged by size:
766 116 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 609 641 888
Size: 609 32 247
Fragments arranged by size:
609 247 32
Tth111I GACn'n_nGTC
Cuts at: 0 193 888
Size: 193 695
Enzymes that do cut:
AarI AccI AfeI AlwNI ApaI BanI BanII BcgI
Bme1580I BplI BpmI BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BstXI Bsu36I BtsI DraIII
EaeI Eco57MI EcoO109I HaeII Hin4I MscI MspA1I NdeI
NspI PflMI PfoI PspOMI PsrI PstI PvuII SfcI
SmlI SphI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI StyI SwaI
XbaI XcmI XhoI XmaI XmnI ZraI