Restriction map for
pFN21AE3908
(Linear) (Six-base) MAPSORT of: KIEE3908.seq Check: 2611 from: 1 to: 1653
KIEE3908.orf
With 182 enzymes: SgfI *
March 22, 2012 16:48 ..
AatII G_ACGT'C
Cuts at: 0 248 1653
Size: 248 1405
AccI GT'mk_AC
Cuts at: 0 662 1653
Size: 662 991
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 1171 1207 1518 1627 1653
Size: 134 1037 36 311 109 26
Fragments arranged by size:
1037 311 134 109 36 26
AflIII A'CryG_T
Cuts at: 0 959 989 995 1064 1653
Size: 959 30 6 69 589
Fragments arranged by size:
959 589 69 30 6
AlwNI CAG_nnn'CTG
Cuts at: 0 1141 1653
Size: 1141 512
ApoI r'AATT_y
Cuts at: 0 1159 1303 1525 1653
Size: 1159 144 222 128
Fragments arranged by size:
1159 222 144 128
BanI G'GyrC_C
Cuts at: 0 844 1100 1653
Size: 844 256 553
Fragments arranged by size:
844 553 256
BbeI G_GCGC'C
Cuts at: 0 848 1104 1653
Size: 848 256 549
Fragments arranged by size:
848 549 256
BbvCI CC'TCA_GC
Cuts at: 0 307 1653
Size: 307 1346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1051 1085 1453 1487 1653
Size: 1051 34 368 34 166
Fragments arranged by size:
1051 368 166 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 392 1653
Size: 392 1261
Bme1580I G_kGCm'C
Cuts at: 0 425 871 989 1653
Size: 425 446 118 664
Fragments arranged by size:
664 446 425 118
BmgBI CAC'GTC
Cuts at: 0 962 1065 1653
Size: 962 103 588
Fragments arranged by size:
962 588 103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 1653
Size: 47 1606
Bpu10I CC'TnA_GC
Cuts at: 0 307 1653
Size: 307 1346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1219 1653
Size: 1219 434
BsaAI yAC'GTr
Cuts at: 0 990 1097 1653
Size: 990 107 556
Fragments arranged by size:
990 556 107
BsaBI GATnn'nnATC
Cuts at: 0 558 1653
Size: 558 1095
BsaHI Gr'CG_yC
Cuts at: 0 245 845 1101 1653
Size: 245 600 256 552
Fragments arranged by size:
600 552 256 245
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1096 1387 1480 1653
Size: 1096 291 93 173
Fragments arranged by size:
1096 291 173 93
BseYI C'CCAG_C
Cuts at: 0 390 1653
Size: 390 1263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1653
Size: 1038 615
BsiHKAI G_wGCw'C
Cuts at: 0 256 1653
Size: 256 1397
BsmI GAATG_Cn'
Cuts at: 0 1476 1653
Size: 1476 177
Bsp1286I G_dGCh'C
Cuts at: 0 256 425 871 989 1653
Size: 256 169 446 118 664
Fragments arranged by size:
664 446 256 169 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 537 1653
Size: 537 1116
BsrDI GCAATG_nn'
Cuts at: 0 171 402 1653
Size: 171 231 1251
Fragments arranged by size:
1251 231 171
BsrGI T'GTAC_A
Cuts at: 0 992 1653
Size: 992 661
BstAPI GCAn_nnn'nTGC
Cuts at: 0 218 1653
Size: 218 1435
BstYI r'GATC_y
Cuts at: 0 553 1653
Size: 553 1100
Bsu36I CC'TnA_GG
Cuts at: 0 1370 1653
Size: 1370 283
BtgI C'CryG_G
Cuts at: 0 1112 1653
Size: 1112 541
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1461 1653
Size: 1461 192
BtsI GCAGTG_nn'
Cuts at: 0 162 1175 1653
Size: 162 1013 478
Fragments arranged by size:
1013 478 162
DraI TTT'AAA
Cuts at: 0 284 1647 1653
Size: 284 1363 6
Fragments arranged by size:
1363 284 6
EaeI y'GGCC_r
Cuts at: 0 91 968 1653
Size: 91 877 685
Fragments arranged by size:
877 685 91
EarI CTCTTCn'nnn_
Cuts at: 0 582 1147 1653
Size: 582 565 506
Fragments arranged by size:
582 565 506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 134 1171 1207 1518 1627 1653
Size: 47 87 1037 36 311 109 26
Fragments arranged by size:
1037 311 109 87 47 36 26
EcoRI G'AATT_C
Cuts at: 0 1525 1653
Size: 1525 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 28 1653
Size: 28 1625
HaeII r_GCGC'y
Cuts at: 0 848 1104 1653
Size: 848 256 549
Fragments arranged by size:
848 549 256
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 1653
Size: 393 32 1228
Fragments arranged by size:
1228 393 32
KasI G'GCGC_C
Cuts at: 0 844 1100 1653
Size: 844 256 553
Fragments arranged by size:
844 553 256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1617 1653
Size: 1617 36
MscI TGG'CCA
Cuts at: 0 93 1653
Size: 93 1560
MspA1I CmG'CkG
Cuts at: 0 390 1653
Size: 390 1263
NarI GG'CG_CC
Cuts at: 0 845 1101 1653
Size: 845 256 552
Fragments arranged by size:
845 552 256
NspI r_CATG'y
Cuts at: 0 635 999 1653
Size: 635 364 654
Fragments arranged by size:
654 635 364
PciI A'CATG_T
Cuts at: 0 995 1653
Size: 995 658
PflMI CCAn_nnn'nTGG
Cuts at: 0 492 1653
Size: 492 1161
PfoI T'CCnGG_A
Cuts at: 0 1331 1653
Size: 1331 322
PmlI CAC'GTG
Cuts at: 0 990 1097 1653
Size: 990 107 556
Fragments arranged by size:
990 556 107
PsiI TTA'TAA
Cuts at: 0 533 1653
Size: 533 1120
PstI C_TGCA'G
Cuts at: 0 1182 1653
Size: 1182 471
PvuII CAG'CTG
Cuts at: 0 390 1653
Size: 390 1263
SfcI C'TryA_G
Cuts at: 0 124 182 1178 1559 1653
Size: 124 58 996 381 94
Fragments arranged by size:
996 381 124 94 58
SfoI GGC'GCC
Cuts at: 0 846 1102 1653
Size: 846 256 551
Fragments arranged by size:
846 551 256
SmlI C'TyrA_G
Cuts at: 0 1234 1653
Size: 1234 419
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1057 1653
Size: 1057 596
TatI w'GTAC_w
Cuts at: 0 45 992 1208 1653
Size: 45 947 216 445
Fragments arranged by size:
947 445 216 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 317 349 1211 1243 1653
Size: 317 32 862 32 410
Fragments arranged by size:
862 410 317 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 394 1653
Size: 394 1259
XmnI GAAnn'nnTTC
Cuts at: 0 11 1653
Size: 11 1642
ZraI GAC'GTC
Cuts at: 0 246 1653
Size: 246 1407
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApoI BanI BbeI
BbvCI BcgI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrGI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI DraI EaeI EarI Eco57MI EcoRI FalI
HaeII Hin4I KasI MmeI MscI MspA1I NarI NspI
PciI PflMI PfoI PmlI PsiI PstI PvuII SfcI
SfoI SmlI TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbsI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI BsaI BsaWI BsaXI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstXI BstZ17I ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
Tth111I XbaI XhoI XmaI