Restriction map for
pFN21AE3907
(Linear) (Six-base) MAPSORT of: KIEE3907.seq Check: 2016 from: 1 to: 1263
KIEE3907.orf
With 182 enzymes: SgfI *
March 22, 2012 16:48 ..
AccI GT'mk_AC
Cuts at: 0 281 1263
Size: 281 982
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 579 664 1263
Size: 520 59 85 599
Fragments arranged by size:
599 520 85 59
AlwNI CAG_nnn'CTG
Cuts at: 0 198 306 930 1263
Size: 198 108 624 333
Fragments arranged by size:
624 333 198 108
ApaI G_GGCC'C
Cuts at: 0 543 1263
Size: 543 720
ApaLI G'TGCA_C
Cuts at: 0 494 1263
Size: 494 769
AvaI C'yCGr_G
Cuts at: 0 844 1174 1263
Size: 844 330 89
Fragments arranged by size:
844 330 89
BanI G'GyrC_C
Cuts at: 0 145 1263
Size: 145 1118
BanII G_rGCy'C
Cuts at: 0 543 836 1263
Size: 543 293 427
Fragments arranged by size:
543 427 293
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1165 1199 1263
Size: 1165 34 64
Fragments arranged by size:
1165 64 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 432 1263
Size: 432 831
Bme1580I G_kGCm'C
Cuts at: 0 498 543 875 963 1263
Size: 498 45 332 88 300
Fragments arranged by size:
498 332 300 88 45
BmgBI CAC'GTC
Cuts at: 0 17 1098 1260 1263
Size: 17 1081 162 3
Fragments arranged by size:
1081 162 17 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 244 996 1263
Size: 193 51 752 267
Fragments arranged by size:
752 267 193 51
Bpu10I CC'TnA_GC
Cuts at: 0 43 1263
Size: 43 1220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1263
Size: 436 827
BsaI GGTCTCn'nnnn_
Cuts at: 0 1016 1263
Size: 1016 247
BsaWI w'CCGG_w
Cuts at: 0 676 1263
Size: 676 587
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 553 583 847 877 1111 1141
Size: 106 30 417 30 264 30 234 30
Cuts at: 1141 1263
Size: 122
Fragments arranged by size:
417 264 234 122 106 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 241 778 855 927 1263
Size: 73 168 537 77 72 336
Fragments arranged by size:
537 336 168 77 73 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 1263
Size: 562 701
BsiEI CG_ry'CG
Cuts at: 0 92 361 1263
Size: 92 269 902
Fragments arranged by size:
902 269 92
BsiHKAI G_wGCw'C
Cuts at: 0 110 233 498 836 1263
Size: 110 123 265 338 427
Fragments arranged by size:
427 338 265 123 110
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1153 1246 1263
Size: 1153 93 17
Fragments arranged by size:
1153 93 17
Bsp1286I G_dGCh'C
Cuts at: 0 110 233 498 543 836 875 963 1263
Size: 110 123 265 45 293 39 88 300
Fragments arranged by size:
300 293 265 123 110 88 45 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1034 1263
Size: 1034 229
BsrDI GCAATG_nn'
Cuts at: 0 1227 1263
Size: 1227 36
BsrFI r'CCGG_y
Cuts at: 0 360 701 1263
Size: 360 341 562
Fragments arranged by size:
562 360 341
BsrGI T'GTAC_A
Cuts at: 0 569 1263
Size: 569 694
BstAPI GCAn_nnn'nTGC
Cuts at: 0 930 1263
Size: 930 333
BstBI TT'CG_AA
Cuts at: 0 687 1263
Size: 687 576
BstEII G'GTnAC_C
Cuts at: 0 414 888 1263
Size: 414 474 375
Fragments arranged by size:
474 414 375
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 1206 1263
Size: 132 1074 57
Fragments arranged by size:
1074 132 57
BstYI r'GATC_y
Cuts at: 0 8 1263
Size: 8 1255
BtgI C'CryG_G
Cuts at: 0 1211 1263
Size: 1211 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 243 1263
Size: 243 1020
BtsI GCAGTG_nn'
Cuts at: 0 532 1081 1263
Size: 532 549 182
Fragments arranged by size:
549 532 182
DraIII CAC_nnn'GTG
Cuts at: 0 988 1263
Size: 988 275
EaeI y'GGCC_r
Cuts at: 0 358 703 1208 1263
Size: 358 345 505 55
Fragments arranged by size:
505 358 345 55
EagI C'GGCC_G
Cuts at: 0 358 1263
Size: 358 905
EarI CTCTTCn'nnn_
Cuts at: 0 124 751 1263
Size: 124 627 512
Fragments arranged by size:
627 512 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 615 1108 1263
Size: 615 493 155
Fragments arranged by size:
615 493 155
EcoICRI GAG'CTC
Cuts at: 0 834 1263
Size: 834 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 244 520 579 664 996 1263
Size: 193 51 276 59 85 332 267
Fragments arranged by size:
332 276 267 193 85 59 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 1030 1263
Size: 55 975 233
Fragments arranged by size:
975 233 55
EcoO109I rG'GnC_Cy
Cuts at: 0 168 336 372 539 1005 1263
Size: 168 168 36 167 466 258
Fragments arranged by size:
466 258 168 168 167 36
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1071 1263
Size: 1071 192
HaeII r_GCGC'y
Cuts at: 0 979 1263
Size: 979 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1224 1256 1263
Size: 1224 32 7
Fragments arranged by size:
1224 32 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 296 1263
Size: 149 147 967
Fragments arranged by size:
967 149 147
MslI CAynn'nnrTG
Cuts at: 0 52 1263
Size: 52 1211
MspA1I CmG'CkG
Cuts at: 0 180 411 588 1263
Size: 180 231 177 675
Fragments arranged by size:
675 231 180 177
NaeI GCC'GGC
Cuts at: 0 362 703 1263
Size: 362 341 560
Fragments arranged by size:
560 362 341
NgoMIV G'CCGG_C
Cuts at: 0 360 701 1263
Size: 360 341 562
Fragments arranged by size:
562 360 341
NruI TCG'CGA
Cuts at: 0 1060 1263
Size: 1060 203
PasI CC'CwG_GG
Cuts at: 0 459 1263
Size: 459 804
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 549 1263
Size: 198 351 714
Fragments arranged by size:
714 351 198
PfoI T'CCnGG_A
Cuts at: 0 131 779 1148 1263
Size: 131 648 369 115
Fragments arranged by size:
648 369 131 115
PpuMI rG'GwC_Cy
Cuts at: 0 168 1263
Size: 168 1095
PspOMI G'GGCC_C
Cuts at: 0 539 1263
Size: 539 724
PstI C_TGCA'G
Cuts at: 0 71 302 1088 1115 1263
Size: 71 231 786 27 148
Fragments arranged by size:
786 231 148 71 27
PvuII CAG'CTG
Cuts at: 0 588 1263
Size: 588 675
SacI G_AGCT'C
Cuts at: 0 836 1263
Size: 836 427
SapI GCTCTTCn'nnn_
Cuts at: 0 751 1263
Size: 751 512
ScaI AGT'ACT
Cuts at: 0 244 1263
Size: 244 1019
SfcI C'TryA_G
Cuts at: 0 67 298 1084 1111 1263
Size: 67 231 786 27 152
Fragments arranged by size:
786 231 152 67 27
SmaI CCC'GGG
Cuts at: 0 846 1263
Size: 846 417
SmlI C'TyrA_G
Cuts at: 0 451 1263
Size: 451 812
StuI AGG'CCT
Cuts at: 0 1051 1263
Size: 1051 212
StyI C'CwwG_G
Cuts at: 0 74 1046 1263
Size: 74 972 217
Fragments arranged by size:
972 217 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 133 1115 1263
Size: 133 982 148
Fragments arranged by size:
982 148 133
TatI w'GTAC_w
Cuts at: 0 242 569 1263
Size: 242 327 694
Fragments arranged by size:
694 327 242
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 106 138 1111 1143 1263
Size: 106 32 973 32 120
Fragments arranged by size:
973 120 106 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1075 1099 1263
Size: 1075 24 164
Fragments arranged by size:
1075 164 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 513 1263
Size: 513 750
XmaI C'CCGG_G
Cuts at: 0 844 1263
Size: 844 419
XmnI GAAnn'nnTTC
Cuts at: 0 244 1263
Size: 244 1019
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApaLI AvaI BanI BanII
BcgI BciVI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
MmeI MslI MspA1I NaeI NgoMIV NruI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SapI ScaI
SfcI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI BsaAI BsaBI
BsaHI BseYI BsiWI BsmI BspEI BspHI BsrBI BssHII
BssSI BstZ17I Bsu36I ClaI CspCI DraI DrdI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NcoI NdeI NheI
NotI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI