Restriction map for
pFN21AE3906
(Linear) (Six-base) MAPSORT of: KIEE3906.seq Check: 5438 from: 1 to: 1377
KIEE3906.orf
With 182 enzymes: SgfI *
March 22, 2012 16:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1170 1377
Size: 1170 207
AccI GT'mk_AC
Cuts at: 0 854 1377
Size: 854 523
Acc65I G'GTAC_C
Cuts at: 0 1184 1377
Size: 1184 193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 917 1023 1377
Size: 267 650 106 354
Fragments arranged by size:
650 354 267 106
AflIII A'CryG_T
Cuts at: 0 857 992 1377
Size: 857 135 385
Fragments arranged by size:
857 385 135
AleI CACnn'nnGTG
Cuts at: 0 651 653 1377
Size: 651 2 724
Fragments arranged by size:
724 651 2
AlwNI CAG_nnn'CTG
Cuts at: 0 1335 1377
Size: 1335 42
ApaLI G'TGCA_C
Cuts at: 0 1022 1377
Size: 1022 355
ApoI r'AATT_y
Cuts at: 0 593 1377
Size: 593 784
BamHI G'GATC_C
Cuts at: 0 1110 1377
Size: 1110 267
BanI G'GyrC_C
Cuts at: 0 1184 1377
Size: 1184 193
BbsI GAAGACnn'nnnn_
Cuts at: 0 1370 1377
Size: 1370 7
BbvCI CC'TCA_GC
Cuts at: 0 197 1377
Size: 197 1180
BciVI GTATCCnnnnn_n'
Cuts at: 0 433 1377
Size: 433 944
BglII A'GATC_T
Cuts at: 0 1282 1377
Size: 1282 95
Bme1580I G_kGCm'C
Cuts at: 0 1026 1377
Size: 1026 351
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1231 1377
Size: 1231 146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1271 1377
Size: 147 1124 106
Fragments arranged by size:
1124 147 106
Bpu10I CC'TnA_GC
Cuts at: 0 197 692 1377
Size: 197 495 685
Fragments arranged by size:
685 495 197
BsaI GGTCTCn'nnnn_
Cuts at: 0 688 1377
Size: 688 689
BsaAI yAC'GTr
Cuts at: 0 435 1377
Size: 435 942
BsaHI Gr'CG_yC
Cuts at: 0 1298 1377
Size: 1298 79
BsaWI w'CCGG_w
Cuts at: 0 1113 1181 1377
Size: 1113 68 196
Fragments arranged by size:
1113 196 68
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 862 892 1377
Size: 862 30 485
Fragments arranged by size:
862 485 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1377
Size: 728 649
BsiHKAI G_wGCw'C
Cuts at: 0 434 1026 1377
Size: 434 592 351
Fragments arranged by size:
592 434 351
BsmI GAATG_Cn'
Cuts at: 0 1245 1377
Size: 1245 132
Bsp1286I G_dGCh'C
Cuts at: 0 434 1026 1377
Size: 434 592 351
Fragments arranged by size:
592 434 351
BspEI T'CCGG_A
Cuts at: 0 1113 1377
Size: 1113 264
BspHI T'CATG_A
Cuts at: 0 1137 1377
Size: 1137 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1170 1377
Size: 1170 207
BsrDI GCAATG_nn'
Cuts at: 0 398 686 1377
Size: 398 288 691
Fragments arranged by size:
691 398 288
BsrGI T'GTAC_A
Cuts at: 0 794 1377
Size: 794 583
BssSI C'ACGA_G
Cuts at: 0 817 1377
Size: 817 560
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1107 1377
Size: 1107 270
BstYI r'GATC_y
Cuts at: 0 1110 1282 1377
Size: 1110 172 95
Fragments arranged by size:
1110 172 95
BtgI C'CryG_G
Cuts at: 0 1301 1377
Size: 1301 76
BtsI GCAGTG_nn'
Cuts at: 0 742 1167 1377
Size: 742 425 210
Fragments arranged by size:
742 425 210
EaeI y'GGCC_r
Cuts at: 0 841 1377
Size: 841 536
EarI CTCTTCn'nnn_
Cuts at: 0 135 561 1377
Size: 135 426 816
Fragments arranged by size:
816 426 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 267 917 1023 1271 1377
Size: 147 120 650 106 248 106
Fragments arranged by size:
650 248 147 120 106 106
EcoO109I rG'GnC_Cy
Cuts at: 0 239 1377
Size: 239 1138
EcoRV GAT'ATC
Cuts at: 0 1005 1377
Size: 1005 372
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 789 1377
Size: 789 588
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 520 552 862 894 1377
Size: 520 32 310 32 483
Fragments arranged by size:
520 483 310 32 32
HincII GTy'rAC
Cuts at: 0 551 1377
Size: 551 826
KpnI G_GTAC'C
Cuts at: 0 1188 1377
Size: 1188 189
MfeI C'AATT_G
Cuts at: 0 1123 1377
Size: 1123 254
MslI CAynn'nnrTG
Cuts at: 0 651 653 1276 1377
Size: 651 2 623 101
Fragments arranged by size:
651 623 101 2
MspA1I CmG'CkG
Cuts at: 0 228 800 1377
Size: 228 572 577
Fragments arranged by size:
577 572 228
NcoI C'CATG_G
Cuts at: 0 1301 1377
Size: 1301 76
NdeI CA'TA_TG
Cuts at: 0 479 1068 1377
Size: 479 589 309
Fragments arranged by size:
589 479 309
NspI r_CATG'y
Cuts at: 0 784 861 996 1377
Size: 784 77 135 381
Fragments arranged by size:
784 381 135 77
PciI A'CATG_T
Cuts at: 0 857 992 1377
Size: 857 135 385
Fragments arranged by size:
857 385 135
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 1377
Size: 345 1032
PfoI T'CCnGG_A
Cuts at: 0 215 1377
Size: 215 1162
PpuMI rG'GwC_Cy
Cuts at: 0 239 1377
Size: 239 1138
PvuII CAG'CTG
Cuts at: 0 800 1377
Size: 800 577
SapI GCTCTTCn'nnn_
Cuts at: 0 561 1377
Size: 561 816
SfcI C'TryA_G
Cuts at: 0 518 1377
Size: 518 859
StyI C'CwwG_G
Cuts at: 0 383 1251 1301 1377
Size: 383 868 50 76
Fragments arranged by size:
868 383 76 50
TatI w'GTAC_w
Cuts at: 0 103 794 1034 1377
Size: 103 691 240 343
Fragments arranged by size:
691 343 240 103
XbaI T'CTAG_A
Cuts at: 0 880 1377
Size: 880 497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 1377
Size: 342 1035
XmnI GAAnn'nnTTC
Cuts at: 0 611 1133 1377
Size: 611 522 244
Fragments arranged by size:
611 522 244
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlwNI ApaLI
ApoI BamHI BanI BbsI BbvCI BciVI BglII Bme1580I
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI
BsrGI BssSI BstXI BstYI BtgI BtsI EaeI EarI
Eco57MI EcoO109I EcoRV FalI Hin4I HincII KpnI MfeI
MslI MspA1I NcoI NdeI NspI PciI PflMI PfoI
PpuMI PvuII SapI SfcI StyI TatI XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AseI AsiSI AvaI AvrII BaeI BanII
BbeI BcgI BclI BfrBI BglI BlpI BmgBI BmrI
BmtI BpuEI BsaBI BseRI BseYI BsiEI BsiWI BsmBI
BsrBI BsrFI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HindIII
HpaI KasI MluI MmeI MscI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XhoI XmaI ZraI