Restriction map for
pFN21AE3905
(Linear) (Six-base) MAPSORT of: KIEE3905.seq Check: 109 from: 1 to: 1335
KIEE3905.orf
With 182 enzymes: SgfI *
March 22, 2012 16:48 ..
Acc65I G'GTAC_C
Cuts at: 0 739 1161 1335
Size: 739 422 174
Fragments arranged by size:
739 422 174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 386 402 830 1335
Size: 335 51 16 428 505
Fragments arranged by size:
505 428 335 51 16
AflIII A'CryG_T
Cuts at: 0 1237 1335
Size: 1237 98
ApoI r'AATT_y
Cuts at: 0 424 520 1135 1335
Size: 424 96 615 200
Fragments arranged by size:
615 424 200 96
AseI AT'TA_AT
Cuts at: 0 617 1335
Size: 617 718
AvaI C'yCGr_G
Cuts at: 0 187 1335
Size: 187 1148
BanI G'GyrC_C
Cuts at: 0 31 739 1161 1335
Size: 31 708 422 174
Fragments arranged by size:
708 422 174 31
BanII G_rGCy'C
Cuts at: 0 1115 1335
Size: 1115 220
BbsI GAAGACnn'nnnn_
Cuts at: 0 499 1335
Size: 499 836
BbvCI CC'TCA_GC
Cuts at: 0 910 1335
Size: 910 425
BciVI GTATCCnnnnn_n'
Cuts at: 0 61 1335
Size: 61 1274
BclI T'GATC_A
Cuts at: 0 890 1335
Size: 890 445
BglII A'GATC_T
Cuts at: 0 1190 1335
Size: 1190 145
Bme1580I G_kGCm'C
Cuts at: 0 34 1335
Size: 34 1301
BmgBI CAC'GTC
Cuts at: 0 18 1335
Size: 18 1317
Bpu10I CC'TnA_GC
Cuts at: 0 910 1335
Size: 910 425
BsaAI yAC'GTr
Cuts at: 0 858 1335
Size: 858 477
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 134 1335
Size: 22 112 1201
Fragments arranged by size:
1201 112 22
BseYI C'CCAG_C
Cuts at: 0 27 850 1335
Size: 27 823 485
Fragments arranged by size:
823 485 27
BsiHKAI G_wGCw'C
Cuts at: 0 1115 1335
Size: 1115 220
Bsp1286I G_dGCh'C
Cuts at: 0 34 1115 1335
Size: 34 1081 220
Fragments arranged by size:
1081 220 34
BsrDI GCAATG_nn'
Cuts at: 0 1046 1335
Size: 1046 289
BsrFI r'CCGG_y
Cuts at: 0 34 1335
Size: 34 1301
BsrGI T'GTAC_A
Cuts at: 0 777 1335
Size: 777 558
BstYI r'GATC_y
Cuts at: 0 459 1190 1335
Size: 459 731 145
Fragments arranged by size:
731 459 145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1209 1244 1335
Size: 1209 35 91
Fragments arranged by size:
1209 91 35
DraI TTT'AAA
Cuts at: 0 168 306 1335
Size: 168 138 1029
Fragments arranged by size:
1029 168 138
EarI CTCTTCn'nnn_
Cuts at: 0 201 1335
Size: 201 1134
EcoICRI GAG'CTC
Cuts at: 0 1113 1335
Size: 1113 222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 386 402 830 1335
Size: 335 51 16 428 505
Fragments arranged by size:
505 428 335 51 16
EcoRV GAT'ATC
Cuts at: 0 993 1335
Size: 993 342
FspI TGC'GCA
Cuts at: 0 1019 1335
Size: 1019 316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 928 960 1335
Size: 115 32 781 32 375
Fragments arranged by size:
781 375 115 32 32
HincII GTy'rAC
Cuts at: 0 222 1335
Size: 222 1113
KpnI G_GTAC'C
Cuts at: 0 743 1165 1335
Size: 743 422 170
Fragments arranged by size:
743 422 170
MfeI C'AATT_G
Cuts at: 0 1124 1335
Size: 1124 211
MspA1I CmG'CkG
Cuts at: 0 258 1335
Size: 258 1077
NdeI CA'TA_TG
Cuts at: 0 95 1209 1335
Size: 95 1114 126
Fragments arranged by size:
1114 126 95
PflMI CCAn_nnn'nTGG
Cuts at: 0 685 1335
Size: 685 650
PvuII CAG'CTG
Cuts at: 0 258 1335
Size: 258 1077
SacI G_AGCT'C
Cuts at: 0 1115 1335
Size: 1115 220
SfcI C'TryA_G
Cuts at: 0 1052 1335
Size: 1052 283
SnaBI TAC'GTA
Cuts at: 0 858 1335
Size: 858 477
StyI C'CwwG_G
Cuts at: 0 690 705 1076 1335
Size: 690 15 371 259
Fragments arranged by size:
690 371 259 15
TatI w'GTAC_w
Cuts at: 0 777 1043 1060 1335
Size: 777 266 17 275
Fragments arranged by size:
777 275 266 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 240 1335
Size: 240 1095
Enzymes that do cut:
Acc65I AcuI AflIII ApoI AseI AvaI BanI BanII
BbsI BbvCI BciVI BclI BglII Bme1580I BmgBI Bpu10I
BsaAI BseRI BseYI BsiHKAI Bsp1286I BsrDI BsrFI BsrGI
BstYI CspCI DraI EarI EcoICRI Eco57MI EcoRV FspI
Hin4I HincII KpnI MfeI MspA1I NdeI PflMI PvuII
SacI SfcI SnaBI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbeI BcgI BfrBI BglI BlpI
BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI FalI
FseI FspAI HaeII HindIII HpaI KasI MluI MmeI
MscI MslI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI