Restriction map for
pFN21AE3903
(Linear) (Six-base) MAPSORT of: KIEE3903.seq Check: 4009 from: 1 to: 1395
KIEE3903.orf
With 182 enzymes: SgfI *
March 22, 2012 16:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 195 1056 1395
Size: 195 861 339
Fragments arranged by size:
861 339 195
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 577 1355 1395
Size: 122 455 778 40
Fragments arranged by size:
778 455 122 40
AflII C'TTAA_G
Cuts at: 0 1330 1395
Size: 1330 65
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 521 1395
Size: 521 874
AlwNI CAG_nnn'CTG
Cuts at: 0 439 1395
Size: 439 956
ApaI G_GGCC'C
Cuts at: 0 1060 1395
Size: 1060 335
AvaI C'yCGr_G
Cuts at: 0 323 1395
Size: 323 1072
BanI G'GyrC_C
Cuts at: 0 218 465 1395
Size: 218 247 930
Fragments arranged by size:
930 247 218
BanII G_rGCy'C
Cuts at: 0 1060 1395
Size: 1060 335
BbeI G_GCGC'C
Cuts at: 0 222 1395
Size: 222 1173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1288 1322 1395
Size: 1288 34 73
Fragments arranged by size:
1288 73 34
BclI T'GATC_A
Cuts at: 0 414 1395
Size: 414 981
BfrBI ATG'CAT
Cuts at: 0 1247 1395
Size: 1247 148
BglI GCCn_nnn'nGGC
Cuts at: 0 945 1395
Size: 945 450
Bme1580I G_kGCm'C
Cuts at: 0 1060 1395
Size: 1060 335
BmrI ACTGGGnnnn_n'
Cuts at: 0 1147 1395
Size: 1147 248
BsaBI GATnn'nnATC
Cuts at: 0 969 1395
Size: 969 426
BsaHI Gr'CG_yC
Cuts at: 0 219 1395
Size: 219 1176
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 545 575 813 840 843 870 1395
Size: 545 30 238 27 3 27 525
Fragments arranged by size:
545 525 238 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 572 668 692 1395
Size: 331 241 96 24 703
Fragments arranged by size:
703 331 241 96 24
BseYI C'CCAG_C
Cuts at: 0 383 974 1006 1395
Size: 383 591 32 389
Fragments arranged by size:
591 389 383 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 471 1395
Size: 173 298 924
Fragments arranged by size:
924 298 173
BsiEI CG_ry'CG
Cuts at: 0 757 1395
Size: 757 638
BsmI GAATG_Cn'
Cuts at: 0 396 634 1395
Size: 396 238 761
Fragments arranged by size:
761 396 238
Bsp1286I G_dGCh'C
Cuts at: 0 1060 1395
Size: 1060 335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 195 513 1056 1395
Size: 195 318 543 339
Fragments arranged by size:
543 339 318 195
BssSI C'ACGA_G
Cuts at: 0 1154 1297 1395
Size: 1154 143 98
Fragments arranged by size:
1154 143 98
BtgI C'CryG_G
Cuts at: 0 106 1395
Size: 106 1289
BtsI GCAGTG_nn'
Cuts at: 0 1058 1395
Size: 1058 337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 577 1355 1395
Size: 122 455 778 40
Fragments arranged by size:
778 455 122 40
EcoO109I rG'GnC_Cy
Cuts at: 0 525 1113 1395
Size: 525 588 282
Fragments arranged by size:
588 525 282
HaeII r_GCGC'y
Cuts at: 0 45 222 1395
Size: 45 177 1173
Fragments arranged by size:
1173 177 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 55 61 87 103 135 229 261
Size: 29 26 6 26 16 32 94 32
Cuts at: 261 545 577 1246 1278 1395
Size: 284 32 669 32 117
Fragments arranged by size:
669 284 117 94 32 32 32 32
29 26 26 16 6
HincII GTy'rAC
Cuts at: 0 405 1320 1395
Size: 405 915 75
Fragments arranged by size:
915 405 75
KasI G'GCGC_C
Cuts at: 0 218 1395
Size: 218 1177
NarI GG'CG_CC
Cuts at: 0 219 1395
Size: 219 1176
NcoI C'CATG_G
Cuts at: 0 106 1395
Size: 106 1289
NdeI CA'TA_TG
Cuts at: 0 627 1020 1395
Size: 627 393 375
Fragments arranged by size:
627 393 375
NsiI A_TGCA'T
Cuts at: 0 1249 1395
Size: 1249 146
NspI r_CATG'y
Cuts at: 0 602 1251 1395
Size: 602 649 144
Fragments arranged by size:
649 602 144
PpuMI rG'GwC_Cy
Cuts at: 0 525 1395
Size: 525 870
PspOMI G'GGCC_C
Cuts at: 0 1056 1395
Size: 1056 339
PspXI vC'TCGA_Gb
Cuts at: 0 323 1395
Size: 323 1072
PstI C_TGCA'G
Cuts at: 0 1053 1395
Size: 1053 342
PvuI CG_AT'CG
Cuts at: 0 757 1395
Size: 757 638
SfcI C'TryA_G
Cuts at: 0 570 1049 1395
Size: 570 479 346
Fragments arranged by size:
570 479 346
SfoI GGC'GCC
Cuts at: 0 220 1395
Size: 220 1175
SmlI C'TyrA_G
Cuts at: 0 323 1330 1395
Size: 323 1007 65
Fragments arranged by size:
1007 323 65
StyI C'CwwG_G
Cuts at: 0 106 1395
Size: 106 1289
TatI w'GTAC_w
Cuts at: 0 514 1395
Size: 514 881
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 509 524 541 556 1395
Size: 509 15 17 15 839
Fragments arranged by size:
839 509 17 15 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 104 1395
Size: 104 1291
XhoI C'TCGA_G
Cuts at: 0 323 1395
Size: 323 1072
XmnI GAAnn'nnTTC
Cuts at: 0 803 1184 1395
Size: 803 381 211
Fragments arranged by size:
803 381 211
Enzymes that do cut:
AarI AcuI AflII AlfI AlwNI ApaI AvaI BanI
BanII BbeI BcgI BclI BfrBI BglI Bme1580I BmrI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmI
Bsp1286I BspMI BssSI BtgI BtsI Eco57MI EcoO109I HaeII
Hin4I HincII KasI NarI NcoI NdeI NsiI NspI
PpuMI PspOMI PspXI PstI PvuI SfcI SfoI SmlI
StyI TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BciVI BglII BlpI BmgBI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PsrI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XmaI ZraI