(Linear) (Six-base) MAPSORT of: KIEE3902.seq  Check: 4546  from: 1  to: 543



KIEE3902.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:47  ..

AccI GT'mk_AC

Cuts at:      0     275     543 
   Size:        275     268
 
ApoI r'AATT_y

Cuts at:      0      56     184     420     543 
   Size:         56     128     236     123

  Fragments arranged by size: 

                 236     128     123      56
 
AvaI C'yCGr_G

Cuts at:      0      44     543 
   Size:         44     499
 
BanI G'GyrC_C

Cuts at:      0     487     543 
   Size:        487      56
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     543 
   Size:        252     291
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     284     543 
   Size:        284     259
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     110     140     543 
   Size:        110      30     403

  Fragments arranged by size: 

                 403     110      30
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     154     543 
   Size:        154     389
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     444     543 
   Size:        444      99
 
BsrDI GCAATG_nn'

Cuts at:      0     512     543 
   Size:        512      31
 
BsrFI r'CCGG_y

Cuts at:      0     490     543 
   Size:        490      53
 
BsrGI T'GTAC_A

Cuts at:      0     101     543 
   Size:        101     442
 
BstEII G'GTnAC_C

Cuts at:      0     431     543 
   Size:        431     112
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     363     543 
   Size:        363     180
 
EarI CTCTTCn'nnn_

Cuts at:      0     335     543 
   Size:        335     208
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     543 
   Size:        252     291
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     499     543 
   Size:        499      44
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     543 
   Size:        311     232
 
MslI CAynn'nnrTG

Cuts at:      0     361     543 
   Size:        361     182
 
SapI GCTCTTCn'nnn_

Cuts at:      0     335     543 
   Size:        335     208
 
SmlI C'TyrA_G

Cuts at:      0      15     543 
   Size:         15     528
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     302     308     464     543 
   Size:        302       6     156      79

  Fragments arranged by size: 

                 302     156      79       6
 
TatI w'GTAC_w

Cuts at:      0     101     543 
   Size:        101     442
 

 Enzymes that do cut:

     AccI     ApoI     AvaI     BanI     BpmI     BsaI    BsaXI    BsmBI
    BspMI    BsrDI    BsrFI    BsrGI   BstEII    BstXI     EarI  Eco57MI
    EcoNI     MmeI     MslI     SapI     SmlI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BsrBI   BssHII
    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI