(Linear) (Six-base) MAPSORT of: KIEE3895.seq  Check: 6393  from: 1  to: 369



KIEE3895.orf

 With 182 enzymes: SgfI * 

                        August 4, 2021 20:20  ..

AccI GT'mk_AC

Cuts at:      0      83     369 
   Size:         83     286
 
AvaI C'yCGr_G

Cuts at:      0      75     369 
   Size:         75     294
 
BanI G'GyrC_C

Cuts at:      0       9     369 
   Size:          9     360
 
BanII G_rGCy'C

Cuts at:      0      97     218     369 
   Size:         97     121     151

  Fragments arranged by size: 

                 151     121      97
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     202     228     296     369 
   Size:        202      26      68      73

  Fragments arranged by size: 

                 202      73      68      26
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     248     277     282     311     369 
   Size:        248      29       5      29      58

  Fragments arranged by size: 

                 248      58      29      29       5
 
BclI T'GATC_A

Cuts at:      0     146     369 
   Size:        146     223
 
BglII A'GATC_T

Cuts at:      0     107     369 
   Size:        107     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     304     369 
   Size:        154     150      65

  Fragments arranged by size: 

                 154     150      65
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225     369 
   Size:        225     144
 
BsaAI yAC'GTr

Cuts at:      0     327     369 
   Size:        327      42
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      31      61     369 
   Size:         31      30     308

  Fragments arranged by size: 

                 308      31      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     218     369 
   Size:        218     151
 
Bsp1286I G_dGCh'C

Cuts at:      0      97     218     369 
   Size:         97     121     151

  Fragments arranged by size: 

                 151     121      97
 
BsrFI r'CCGG_y

Cuts at:      0       6     369 
   Size:          6     363
 
BssHII G'CGCG_C

Cuts at:      0      30     369 
   Size:         30     339
 
BstEII G'GTnAC_C

Cuts at:      0     153     369 
   Size:        153     216
 
BstYI r'GATC_y

Cuts at:      0     107     369 
   Size:        107     262
 
BtgI C'CryG_G

Cuts at:      0     134     209     369 
   Size:        134      75     160

  Fragments arranged by size: 

                 160     134      75
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     154     369 
   Size:        154     215
 
EcoICRI GAG'CTC

Cuts at:      0     216     369 
   Size:        216     153
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     304     369 
   Size:        154     150      65

  Fragments arranged by size: 

                 154     150      65
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     148     172     369 
   Size:        148      24     197

  Fragments arranged by size: 

                 197     148      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     141     369 
   Size:        141     228
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      31      63     369 
   Size:         31      32     306

  Fragments arranged by size: 

                 306      32      31
 
MspA1I CmG'CkG

Cuts at:      0      15     369 
   Size:         15     354
 
NcoI C'CATG_G

Cuts at:      0     209     369 
   Size:        209     160
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     177     210     369 
   Size:        177      33     159

  Fragments arranged by size: 

                 177     159      33
 
PpuMI rG'GwC_Cy

Cuts at:      0     141     369 
   Size:        141     228
 
PstI C_TGCA'G

Cuts at:      0      20     369 
   Size:         20     349
 
SacI G_AGCT'C

Cuts at:      0     218     369 
   Size:        218     151
 
SfcI C'TryA_G

Cuts at:      0      16     369 
   Size:         16     353
 
SmlI C'TyrA_G

Cuts at:      0     240     369 
   Size:        240     129
 
SnaBI TAC'GTA

Cuts at:      0     327     369 
   Size:        327      42
 
StyI C'CwwG_G

Cuts at:      0      35     209     282     369 
   Size:         35     174      73      87

  Fragments arranged by size: 

                 174      87      73      35
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     172     369 
   Size:        172     197
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     207     369 
   Size:        207     162
 

 Enzymes that do cut:

     AccI     AvaI     BanI    BanII     BbsI     BcgI     BclI    BglII
     BpmI    BpuEI    BsaAI    BsaXI  BsiHKAI Bsp1286I    BsrFI   BssHII
   BstEII    BstYI     BtgI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    Hin4I   MspA1I     NcoI    PflMI    PpuMI     PstI     SacI     SfcI
     SmlI    SnaBI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
    BbvCI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI