Restriction map for
pFN21AE3890
(Linear) (Six-base) MAPSORT of: KIEE3890.seq Check: 5403 from: 1 to: 1917
KIEE3890.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
AccI GT'mk_AC
Cuts at: 0 1376 1917
Size: 1376 541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1118 1609 1917
Size: 1118 491 308
Fragments arranged by size:
1118 491 308
AlwNI CAG_nnn'CTG
Cuts at: 0 544 747 1917
Size: 544 203 1170
Fragments arranged by size:
1170 544 203
ApoI r'AATT_y
Cuts at: 0 184 281 477 629 1306 1425 1740 1917
Size: 184 97 196 152 677 119 315 177
Fragments arranged by size:
677 315 196 184 177 152 119 97
AseI AT'TA_AT
Cuts at: 0 410 1277 1917
Size: 410 867 640
Fragments arranged by size:
867 640 410
BanII G_rGCy'C
Cuts at: 0 1764 1917
Size: 1764 153
BbsI GAAGACnn'nnnn_
Cuts at: 0 325 1580 1861 1904 1917
Size: 325 1255 281 43 13
Fragments arranged by size:
1255 325 281 43 13
BglII A'GATC_T
Cuts at: 0 1207 1917
Size: 1207 710
BmrI ACTGGGnnnn_n'
Cuts at: 0 757 1917
Size: 757 1160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 400 929 1025 1917
Size: 296 104 529 96 892
Fragments arranged by size:
892 529 296 104 96
Bpu10I CC'TnA_GC
Cuts at: 0 1034 1917
Size: 1034 883
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 1917
Size: 91 30 1796
Fragments arranged by size:
1796 91 30
BseYI C'CCAG_C
Cuts at: 0 553 1917
Size: 553 1364
BsmBI CGTCTCn'nnnn_
Cuts at: 0 172 1917
Size: 172 1745
Bsp1286I G_dGCh'C
Cuts at: 0 1764 1917
Size: 1764 153
BspHI T'CATG_A
Cuts at: 0 519 1917
Size: 519 1398
BsrGI T'GTAC_A
Cuts at: 0 36 243 1917
Size: 36 207 1674
Fragments arranged by size:
1674 207 36
BstYI r'GATC_y
Cuts at: 0 1207 1917
Size: 1207 710
BtsI GCAGTG_nn'
Cuts at: 0 1466 1917
Size: 1466 451
DraI TTT'AAA
Cuts at: 0 660 870 1091 1917
Size: 660 210 221 826
Fragments arranged by size:
826 660 221 210
EarI CTCTTCn'nnn_
Cuts at: 0 129 410 704 1844 1917
Size: 129 281 294 1140 73
Fragments arranged by size:
1140 294 281 129 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 400 929 1025 1118 1609 1917
Size: 296 104 529 96 93 491 308
Fragments arranged by size:
529 491 308 296 104 96 93
EcoO109I rG'GnC_Cy
Cuts at: 0 751 1400 1917
Size: 751 649 517
Fragments arranged by size:
751 649 517
EcoRI G'AATT_C
Cuts at: 0 184 281 1917
Size: 184 97 1636
Fragments arranged by size:
1636 184 97
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 877 1917
Size: 877 1040
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 59 91 1099 1131 1309 1341 1854 1886
Size: 59 32 1008 32 178 32 513 32
Cuts at: 1886 1917
Size: 31
Fragments arranged by size:
1008 513 178 59 32 32 32 32
31
HincII GTy'rAC
Cuts at: 0 6 1917
Size: 6 1911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 1555 1917
Size: 45 1510 362
Fragments arranged by size:
1510 362 45
MslI CAynn'nnrTG
Cuts at: 0 44 227 1917
Size: 44 183 1690
Fragments arranged by size:
1690 183 44
MspA1I CmG'CkG
Cuts at: 0 274 1353 1917
Size: 274 1079 564
Fragments arranged by size:
1079 564 274
NspI r_CATG'y
Cuts at: 0 1619 1917
Size: 1619 298
PpuMI rG'GwC_Cy
Cuts at: 0 751 1400 1917
Size: 751 649 517
Fragments arranged by size:
751 649 517
PstI C_TGCA'G
Cuts at: 0 1071 1917
Size: 1071 846
PvuII CAG'CTG
Cuts at: 0 274 1353 1917
Size: 274 1079 564
Fragments arranged by size:
1079 564 274
SanDI GG'GwC_CC
Cuts at: 0 751 1917
Size: 751 1166
SfcI C'TryA_G
Cuts at: 0 94 739 1067 1265 1917
Size: 94 645 328 198 652
Fragments arranged by size:
652 645 328 198 94
SspI AAT'ATT
Cuts at: 0 817 1081 1917
Size: 817 264 836
Fragments arranged by size:
836 817 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1179 1917
Size: 1179 738
TatI w'GTAC_w
Cuts at: 0 36 243 353 1917
Size: 36 207 110 1564
Fragments arranged by size:
1564 207 110 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 91 123 1917
Size: 91 32 1794
Fragments arranged by size:
1794 91 32
XbaI T'CTAG_A
Cuts at: 0 1872 1917
Size: 1872 45
Enzymes that do cut:
AccI AcuI AlwNI ApoI AseI BanII BbsI BglII
BmrI BpmI Bpu10I BsaXI BseYI BsmBI Bsp1286I BspHI
BsrGI BstYI BtsI DraI EarI Eco57MI EcoO109I EcoRI
FalI Hin4I HincII MmeI MslI MspA1I NspI PpuMI
PstI PvuII SanDI SfcI SspI TaqII TatI TstI
XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI SwaI Tth111I XcmI XhoI
XmaI XmnI ZraI