Restriction map for
pFN21AE3888
(Linear) (Six-base) MAPSORT of: KIEE3888.seq Check: 8376 from: 1 to: 912
KIEE3888.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
Acc65I G'GTAC_C
Cuts at: 0 120 912
Size: 120 792
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 912
Size: 599 313
AfeI AGC'GCT
Cuts at: 0 337 912
Size: 337 575
AflIII A'CryG_T
Cuts at: 0 731 912
Size: 731 181
AgeI A'CCGG_T
Cuts at: 0 548 912
Size: 548 364
AleI CACnn'nnGTG
Cuts at: 0 751 817 912
Size: 751 66 95
Fragments arranged by size:
751 95 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 432 912
Size: 432 480
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 912
Size: 265 32 615
Fragments arranged by size:
615 265 32
AlwNI CAG_nnn'CTG
Cuts at: 0 883 912
Size: 883 29
ApaLI G'TGCA_C
Cuts at: 0 488 866 912
Size: 488 378 46
Fragments arranged by size:
488 378 46
ApoI r'AATT_y
Cuts at: 0 370 421 532 912
Size: 370 51 111 380
Fragments arranged by size:
380 370 111 51
AvaI C'yCGr_G
Cuts at: 0 213 696 716 898 912
Size: 213 483 20 182 14
Fragments arranged by size:
483 213 182 20 14
BanI G'GyrC_C
Cuts at: 0 120 912
Size: 120 792
BanII G_rGCy'C
Cuts at: 0 71 912
Size: 71 841
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 912
Size: 248 664
BbvCI CC'TCA_GC
Cuts at: 0 769 912
Size: 769 143
BglI GCCn_nnn'nGGC
Cuts at: 0 190 912
Size: 190 722
Bme1580I G_kGCm'C
Cuts at: 0 492 870 912
Size: 492 378 42
Fragments arranged by size:
492 378 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 169 400 711 886 912
Size: 169 231 311 175 26
Fragments arranged by size:
311 231 175 169 26
Bpu10I CC'TnA_GC
Cuts at: 0 769 876 912
Size: 769 107 36
Fragments arranged by size:
769 107 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 53 912
Size: 53 859
BsaWI w'CCGG_w
Cuts at: 0 548 654 912
Size: 548 106 258
Fragments arranged by size:
548 258 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 223 253 912
Size: 223 30 659
Fragments arranged by size:
659 223 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 265 912
Size: 127 138 647
Fragments arranged by size:
647 138 127
BseYI C'CCAG_C
Cuts at: 0 70 223 912
Size: 70 153 689
Fragments arranged by size:
689 153 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 485 912
Size: 119 366 427
Fragments arranged by size:
427 366 119
BsiHKAI G_wGCw'C
Cuts at: 0 492 870 912
Size: 492 378 42
Fragments arranged by size:
492 378 42
Bsp1286I G_dGCh'C
Cuts at: 0 71 492 870 912
Size: 71 421 378 42
Fragments arranged by size:
421 378 71 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 108 912
Size: 108 804
BsrBI CCG'CTC
Cuts at: 0 303 912
Size: 303 609
BsrDI GCAATG_nn'
Cuts at: 0 351 614 912
Size: 351 263 298
Fragments arranged by size:
351 298 263
BsrFI r'CCGG_y
Cuts at: 0 548 912
Size: 548 364
BstAPI GCAn_nnn'nTGC
Cuts at: 0 463 912
Size: 463 449
BstYI r'GATC_y
Cuts at: 0 540 912
Size: 540 372
Bsu36I CC'TnA_GG
Cuts at: 0 884 912
Size: 884 28
BtsI GCAGTG_nn'
Cuts at: 0 595 912
Size: 595 317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 123 158 912
Size: 123 35 754
Fragments arranged by size:
754 123 35
DraIII CAC_nnn'GTG
Cuts at: 0 20 130 912
Size: 20 110 782
Fragments arranged by size:
782 110 20
EaeI y'GGCC_r
Cuts at: 0 796 912
Size: 796 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 912
Size: 599 313
EcoO109I rG'GnC_Cy
Cuts at: 0 880 912
Size: 880 32
EcoRV GAT'ATC
Cuts at: 0 150 912
Size: 150 762
HaeII r_GCGC'y
Cuts at: 0 291 339 912
Size: 291 48 573
Fragments arranged by size:
573 291 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 912
Size: 223 32 657
Fragments arranged by size:
657 223 32
HincII GTy'rAC
Cuts at: 0 159 360 912
Size: 159 201 552
Fragments arranged by size:
552 201 159
KpnI G_GTAC'C
Cuts at: 0 124 912
Size: 124 788
MscI TGG'CCA
Cuts at: 0 798 912
Size: 798 114
MslI CAynn'nnrTG
Cuts at: 0 751 817 912
Size: 751 66 95
Fragments arranged by size:
751 95 66
MspA1I CmG'CkG
Cuts at: 0 201 591 912
Size: 201 390 321
Fragments arranged by size:
390 321 201
NspI r_CATG'y
Cuts at: 0 735 912
Size: 735 177
PasI CC'CwG_GG
Cuts at: 0 42 912
Size: 42 870
PciI A'CATG_T
Cuts at: 0 731 912
Size: 731 181
PflMI CCAn_nnn'nTGG
Cuts at: 0 130 912
Size: 130 782
PvuII CAG'CTG
Cuts at: 0 201 912
Size: 201 711
SfcI C'TryA_G
Cuts at: 0 777 912
Size: 777 135
SmaI CCC'GGG
Cuts at: 0 718 912
Size: 718 194
SpeI A'CTAG_T
Cuts at: 0 630 912
Size: 630 282
StuI AGG'CCT
Cuts at: 0 193 793 912
Size: 193 600 119
Fragments arranged by size:
600 193 119
XmaI C'CCGG_G
Cuts at: 0 716 912
Size: 716 196
XmnI GAAnn'nnTTC
Cuts at: 0 733 912
Size: 733 179
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AgeI AleI AlfI AloI
AlwNI ApaLI ApoI AvaI BanI BanII BbsI BbvCI
BglI Bme1580I BplI Bpu10I BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstAPI BstYI Bsu36I BtsI CspCI DraIII EaeI Eco57MI
EcoO109I EcoRV HaeII Hin4I HincII KpnI MscI MslI
MspA1I NspI PasI PciI PflMI PvuII SfcI SmaI
SpeI StuI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AhdI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpmI
BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I BtgI BtgZI ClaI DraI DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SphI SrfI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI ZraI