(Linear) (Six-base) MAPSORT of: KIEE3887.seq  Check: 5963  from: 1  to: 1026



KIEE3887.orf

 With 182 enzymes: SgfI * 

                        August 4, 2021 20:20  ..

AclI AA'CG_TT

Cuts at:      0     321    1026 
   Size:        321     705
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595     793    1026 
   Size:        595     198     233

  Fragments arranged by size: 

                 595     233     198
 
AflIII A'CryG_T

Cuts at:      0     909    1026 
   Size:        909     117
 
ApoI r'AATT_y

Cuts at:      0     568     763    1026 
   Size:        568     195     263

  Fragments arranged by size: 

                 568     263     195
 
BanII G_rGCy'C

Cuts at:      0      98    1026 
   Size:         98     928
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     458    1026 
   Size:        458     568
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     871    1026 
   Size:        871     155
 
BclI T'GATC_A

Cuts at:      0     402     540    1026 
   Size:        402     138     486

  Fragments arranged by size: 

                 486     402     138
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464    1026 
   Size:        464     562
 
BsaAI yAC'GTr

Cuts at:      0     786    1026 
   Size:        786     240
 
BsaBI GATnn'nnATC

Cuts at:      0     803    1026 
   Size:        803     223
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     287    1026 
   Size:        172     115     739

  Fragments arranged by size: 

                 739     172     115
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661    1026 
   Size:        661     365
 
BsiHKAI G_wGCw'C

Cuts at:      0      98    1026 
   Size:         98     928
 
BsmI GAATG_Cn'

Cuts at:      0     615    1026 
   Size:        615     411
 
Bsp1286I G_dGCh'C

Cuts at:      0      98    1026 
   Size:         98     928
 
BspHI T'CATG_A

Cuts at:      0     477    1026 
   Size:        477     549
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      65    1026 
   Size:         65     961
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     799    1026 
   Size:        799     227
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1008    1026 
   Size:       1008      18
 
BstYI r'GATC_y

Cuts at:      0      20     338    1026 
   Size:         20     318     688

  Fragments arranged by size: 

                 688     318      20
 
Bsu36I CC'TnA_GG

Cuts at:      0     525    1026 
   Size:        525     501
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     551    1026 
   Size:        551     475
 
BtsI GCAGTG_nn'

Cuts at:      0     642    1026 
   Size:        642     384
 
DraI TTT'AAA

Cuts at:      0     917    1026 
   Size:        917     109
 
EaeI y'GGCC_r

Cuts at:      0     815    1026 
   Size:        815     211
 
EarI CTCTTCn'nnn_

Cuts at:      0     104     494     949    1026 
   Size:        104     390     455      77

  Fragments arranged by size: 

                 455     390     104      77
 
EcoICRI GAG'CTC

Cuts at:      0      96    1026 
   Size:         96     930
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464     595     793    1026 
   Size:        464     131     198     233

  Fragments arranged by size: 

                 464     233     198     131
 
HaeII r_GCGC'y

Cuts at:      0     514    1026 
   Size:        514     512
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     266     298    1026 
   Size:        266      32     728

  Fragments arranged by size: 

                 728     266      32
 
HincII GTy'rAC

Cuts at:      0     357    1026 
   Size:        357     669
 
HpaI GTT'AAC

Cuts at:      0     357    1026 
   Size:        357     669
 
MfeI C'AATT_G

Cuts at:      0     494    1026 
   Size:        494     532
 
MscI TGG'CCA

Cuts at:      0     817    1026 
   Size:        817     209
 
MslI CAynn'nnrTG

Cuts at:      0    1002    1026 
   Size:       1002      24
 
MspA1I CmG'CkG

Cuts at:      0      39     933    1026 
   Size:         39     894      93

  Fragments arranged by size: 

                 894      93      39
 
NspI r_CATG'y

Cuts at:      0     904     913    1026 
   Size:        904       9     113

  Fragments arranged by size: 

                 904     113       9
 
PciI A'CATG_T

Cuts at:      0     909    1026 
   Size:        909     117
 
PvuII CAG'CTG

Cuts at:      0      39    1026 
   Size:         39     987
 
SacI G_AGCT'C

Cuts at:      0      98    1026 
   Size:         98     928
 
SfcI C'TryA_G

Cuts at:      0     989    1026 
   Size:        989      37
 
SspI AAT'ATT

Cuts at:      0      79    1026 
   Size:         79     947
 
StyI C'CwwG_G

Cuts at:      0     389     832    1026 
   Size:        389     443     194

  Fragments arranged by size: 

                 443     389     194
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     691    1026 
   Size:        691     335
 
TatI w'GTAC_w

Cuts at:      0    1013    1026 
   Size:       1013      13
 
Tth111I GACn'n_nGTC

Cuts at:      0     678    1026 
   Size:        678     348
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     871    1026 
   Size:        871     155
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     ApoI    BanII     BbsI    BciVI     BclI
     BpmI    BsaAI    BsaBI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI   BstAPI    BstXI    BstYI   Bsu36I    BtgZI     BtsI
     DraI     EaeI     EarI  EcoICRI  Eco57MI    HaeII    Hin4I   HincII
     HpaI     MfeI     MscI     MslI   MspA1I     NspI     PciI    PvuII
     SacI     SfcI     SspI     StyI    TaqII     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I     BtgI     ClaI
    CspCI   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     KasI     KpnI
     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI     XbaI
     XhoI     XmaI     XmnI     ZraI