(Linear) (Six-base) MAPSORT of: KIEE3879.seq  Check: 4610  from: 1  to: 2928



KIEE3879.orf

 With 182 enzymes: SgfI * 

                        August 4, 2021 20:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    1088    1391    1837    2848    2928 
   Size:        146     942     303     446    1011      80

  Fragments arranged by size: 

                1011     942     446     303     146      80
 
AflII C'TTAA_G

Cuts at:      0    1531    2928 
   Size:       1531    1397
 
AlwNI CAG_nnn'CTG

Cuts at:      0      81     196     373     450    2928 
   Size:         81     115     177      77    2478

  Fragments arranged by size: 

                2478     177     115      81      77
 
ApoI r'AATT_y

Cuts at:      0     291     699    1135    1966    2414    2466    2659    2928 
   Size:        291     408     436     831     448      52     193     269

  Fragments arranged by size: 

                 831     448     436     408     291     269     193      52
            
 
AseI AT'TA_AT

Cuts at:      0    1643    2669    2928 
   Size:       1643    1026     259

  Fragments arranged by size: 

                1643    1026     259
 
BanII G_rGCy'C

Cuts at:      0    1589    2928 
   Size:       1589    1339
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1399    2578    2928 
   Size:       1399    1179     350

  Fragments arranged by size: 

                1399    1179     350
 
BclI T'GATC_A

Cuts at:      0    2481    2928 
   Size:       2481     447
 
BfrBI ATG'CAT

Cuts at:      0     717    2928 
   Size:        717    2211
 
BglII A'GATC_T

Cuts at:      0     574    1500    2928 
   Size:        574     926    1428

  Fragments arranged by size: 

                1428     926     574
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250    1606    2928 
   Size:        250    1356    1322

  Fragments arranged by size: 

                1356    1322     250
 
BsaBI GATnn'nnATC

Cuts at:      0     191     820    2928 
   Size:        191     629    2108

  Fragments arranged by size: 

                2108     629     191
 
BsiHKAI G_wGCw'C

Cuts at:      0    1589    2928 
   Size:       1589    1339
 
Bsp1286I G_dGCh'C

Cuts at:      0    1589    2928 
   Size:       1589    1339
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     225     789    2928 
   Size:        225     564    2139

  Fragments arranged by size: 

                2139     564     225
 
BsrDI GCAATG_nn'

Cuts at:      0    2548    2928 
   Size:       2548     380
 
BstYI r'GATC_y

Cuts at:      0     192     574     636    1500    2818    2928 
   Size:        192     382      62     864    1318     110

  Fragments arranged by size: 

                1318     864     382     192     110      62
 
BtgI C'CryG_G

Cuts at:      0    1226    2928 
   Size:       1226    1702
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2118    2928 
   Size:       2118     810
 
BtsI GCAGTG_nn'

Cuts at:      0    1554    2928 
   Size:       1554    1374
 
EarI CTCTTCn'nnn_

Cuts at:      0    1253    1736    2033    2309    2714    2928 
   Size:       1253     483     297     276     405     214

  Fragments arranged by size: 

                1253     483     405     297     276     214
 
EcoICRI GAG'CTC

Cuts at:      0    1587    2928 
   Size:       1587    1341
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    1088    1391    1837    2848    2928 
   Size:        146     942     303     446    1011      80

  Fragments arranged by size: 

                1011     942     446     303     146      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2793    2928 
   Size:       2793     135
 
EcoRI G'AATT_C

Cuts at:      0     291     699    2928 
   Size:        291     408    2229

  Fragments arranged by size: 

                2229     408     291
 
EcoRV GAT'ATC

Cuts at:      0    2018    2928 
   Size:       2018     910
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1312    2047    2293    2928 
   Size:       1312     735     246     635

  Fragments arranged by size: 

                1312     735     635     246
 
HincII GTy'rAC

Cuts at:      0    1454    2110    2928 
   Size:       1454     656     818

  Fragments arranged by size: 

                1454     818     656
 
HindIII A'AGCT_T

Cuts at:      0    1270    1528    1564    2928 
   Size:       1270     258      36    1364

  Fragments arranged by size: 

                1364    1270     258      36
 
HpaI GTT'AAC

Cuts at:      0    1454    2928 
   Size:       1454    1474
 
MfeI C'AATT_G

Cuts at:      0     938    1210    2928 
   Size:        938     272    1718

  Fragments arranged by size: 

                1718     938     272
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     774    2928 
   Size:        774    2154
 
MslI CAynn'nnrTG

Cuts at:      0    2159    2771    2928 
   Size:       2159     612     157

  Fragments arranged by size: 

                2159     612     157
 
NcoI C'CATG_G

Cuts at:      0    1226    2928 
   Size:       1226    1702
 
NsiI A_TGCA'T

Cuts at:      0     719    2928 
   Size:        719    2209
 
PasI CC'CwG_GG

Cuts at:      0      81    2928 
   Size:         81    2847
 
PsiI TTA'TAA

Cuts at:      0    2204    2928 
   Size:       2204     724
 
PstI C_TGCA'G

Cuts at:      0     582    1156    2928 
   Size:        582     574    1772

  Fragments arranged by size: 

                1772     582     574
 
SacI G_AGCT'C

Cuts at:      0    1589    2928 
   Size:       1589    1339
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1736    2928 
   Size:       1736    1192
 
ScaI AGT'ACT

Cuts at:      0    1008    2928 
   Size:       1008    1920
 
SfcI C'TryA_G

Cuts at:      0     578    1152    2593    2928 
   Size:        578     574    1441     335

  Fragments arranged by size: 

                1441     578     574     335
 
SmlI C'TyrA_G

Cuts at:      0     229    1531    1621    2928 
   Size:        229    1302      90    1307

  Fragments arranged by size: 

                1307    1302     229      90
 
StuI AGG'CCT

Cuts at:      0    1870    2928 
   Size:       1870    1058
 
StyI C'CwwG_G

Cuts at:      0    1226    2928 
   Size:       1226    1702
 
TatI w'GTAC_w

Cuts at:      0     121     364    1006    1431    2928 
   Size:        121     243     642     425    1497

  Fragments arranged by size: 

                1497     642     425     243     121
 
XmnI GAAnn'nnTTC

Cuts at:      0    1691    1850    2928 
   Size:       1691     159    1078

  Fragments arranged by size: 

                1691    1078     159
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     ApoI     AseI    BanII     BbsI     BclI
    BfrBI    BglII    BpuEI    BsaBI  BsiHKAI Bsp1286I    BspMI    BsrDI
    BstYI     BtgI    BtgZI     BtsI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI   HincII  HindIII     HpaI     MfeI     MmeI
     MslI     NcoI     NsiI     PasI     PsiI     PstI     SacI     SapI
     ScaI     SfcI     SmlI     StuI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI    EcoNI     FseI     FspI    FspAI    HaeII
    Hin4I     KasI     KpnI     MluI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI