(Linear) (Six-base) MAPSORT of: KIEE3875.seq Check: 9924 from: 1 to: 1056 KIEE3875.orf With 182 enzymes: SgfI * August 4, 2021 20:20 .. AccI GT'mk_AC Cuts at: 0 785 919 998 1056 Size: 785 134 79 58 Fragments arranged by size: 785 134 79 58 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 502 916 1056 Size: 340 162 414 140 Fragments arranged by size: 414 340 162 140 AfeI AGC'GCT Cuts at: 0 11 1056 Size: 11 1045 AflIII A'CryG_T Cuts at: 0 260 465 1056 Size: 260 205 591 Fragments arranged by size: 591 260 205 AlwNI CAG_nnn'CTG Cuts at: 0 41 1056 Size: 41 1015 ApoI r'AATT_y Cuts at: 0 706 949 1056 Size: 706 243 107 Fragments arranged by size: 706 243 107 BamHI G'GATC_C Cuts at: 0 70 1056 Size: 70 986 BanI G'GyrC_C Cuts at: 0 637 1056 Size: 637 419 BanII G_rGCy'C Cuts at: 0 212 353 563 1056 Size: 212 141 210 493 Fragments arranged by size: 493 212 210 141 BbsI GAAGACnn'nnnn_ Cuts at: 0 490 1056 Size: 490 566 BglI GCCn_nnn'nGGC Cuts at: 0 460 1056 Size: 460 596 Bme1580I G_kGCm'C Cuts at: 0 371 1056 Size: 371 685 BmgBI CAC'GTC Cuts at: 0 468 1056 Size: 468 588 BmrI ACTGGGnnnn_n' Cuts at: 0 109 1056 Size: 109 947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 1056 Size: 226 830 BsaAI yAC'GTr Cuts at: 0 263 366 1056 Size: 263 103 690 Fragments arranged by size: 690 263 103 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 601 631 1056 Size: 601 30 425 Fragments arranged by size: 601 425 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 727 769 1056 Size: 727 42 287 Fragments arranged by size: 727 287 42 BseYI C'CCAG_C Cuts at: 0 746 1056 Size: 746 310 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 366 1056 Size: 214 152 690 Fragments arranged by size: 690 214 152 BsiHKAI G_wGCw'C Cuts at: 0 212 353 977 1056 Size: 212 141 624 79 Fragments arranged by size: 624 212 141 79 BsmBI CGTCTCn'nnnn_ Cuts at: 0 474 1056 Size: 474 582 Bsp1286I G_dGCh'C Cuts at: 0 212 353 371 563 977 1056 Size: 212 141 18 192 414 79 Fragments arranged by size: 414 212 192 141 79 18 BspHI T'CATG_A Cuts at: 0 401 1056 Size: 401 655 BssSI C'ACGA_G Cuts at: 0 346 823 1056 Size: 346 477 233 Fragments arranged by size: 477 346 233 BstAPI GCAn_nnn'nTGC Cuts at: 0 237 1056 Size: 237 819 BstXI CCAn_nnnn'nTGG Cuts at: 0 66 1056 Size: 66 990 BstYI r'GATC_y Cuts at: 0 70 446 1056 Size: 70 376 610 Fragments arranged by size: 610 376 70 BtgI C'CryG_G Cuts at: 0 440 1056 Size: 440 616 BtsI GCAGTG_nn' Cuts at: 0 218 1056 Size: 218 838 DraIII CAC_nnn'GTG Cuts at: 0 330 1056 Size: 330 726 EaeI y'GGCC_r Cuts at: 0 56 452 1056 Size: 56 396 604 Fragments arranged by size: 604 396 56 EarI CTCTTCn'nnn_ Cuts at: 0 842 1056 Size: 842 214 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 462 570 1056 Size: 462 108 486 Fragments arranged by size: 486 462 108 EcoICRI GAG'CTC Cuts at: 0 210 351 1056 Size: 210 141 705 Fragments arranged by size: 705 210 141 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 340 502 916 1056 Size: 226 114 162 414 140 Fragments arranged by size: 414 226 162 140 114 EcoNI CCTnn'n_nnAGG Cuts at: 0 567 1056 Size: 567 489 EcoO109I rG'GnC_Cy Cuts at: 0 164 681 1056 Size: 164 517 375 Fragments arranged by size: 517 375 164 EcoRI G'AATT_C Cuts at: 0 949 1056 Size: 949 107 FspI TGC'GCA Cuts at: 0 688 1056 Size: 688 368 HaeII r_GCGC'y Cuts at: 0 13 281 1056 Size: 13 268 775 Fragments arranged by size: 775 268 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 62 94 1056 Size: 62 32 962 Fragments arranged by size: 962 62 32 MscI TGG'CCA Cuts at: 0 58 1056 Size: 58 998 MspA1I CmG'CkG Cuts at: 0 204 660 750 873 987 1056 Size: 204 456 90 123 114 69 Fragments arranged by size: 456 204 123 114 90 69 NcoI C'CATG_G Cuts at: 0 440 1056 Size: 440 616 PfoI T'CCnGG_A Cuts at: 0 554 1056 Size: 554 502 PmlI CAC'GTG Cuts at: 0 263 1056 Size: 263 793 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 952 984 1056 Size: 952 32 72 Fragments arranged by size: 952 72 32 PpuMI rG'GwC_Cy Cuts at: 0 164 1056 Size: 164 892 PstI C_TGCA'G Cuts at: 0 182 872 1056 Size: 182 690 184 Fragments arranged by size: 690 184 182 PvuII CAG'CTG Cuts at: 0 204 873 987 1056 Size: 204 669 114 69 Fragments arranged by size: 669 204 114 69 SacI G_AGCT'C Cuts at: 0 212 353 1056 Size: 212 141 703 Fragments arranged by size: 703 212 141 SanDI GG'GwC_CC Cuts at: 0 164 1056 Size: 164 892 SbfI CC_TGCA'GG Cuts at: 0 182 1056 Size: 182 874 SexAI A'CCwGG_T Cuts at: 0 248 1056 Size: 248 808 SfcI C'TryA_G Cuts at: 0 178 868 1056 Size: 178 690 188 Fragments arranged by size: 690 188 178 StyI C'CwwG_G Cuts at: 0 371 440 1056 Size: 371 69 616 Fragments arranged by size: 616 371 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 128 616 688 760 1056 Size: 128 488 72 72 296 Fragments arranged by size: 488 296 128 72 72 TatI w'GTAC_w Cuts at: 0 428 497 608 1056 Size: 428 69 111 448 Fragments arranged by size: 448 428 111 69 Tth111I GACn'n_nGTC Cuts at: 0 700 1056 Size: 700 356 Enzymes that do cut: AccI AcuI AfeI AflIII AlwNI ApoI BamHI BanI BanII BbsI BglI Bme1580I BmgBI BmrI BpmI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BssSI BstAPI BstXI BstYI BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I MscI MspA1I NcoI PfoI PmlI PpiI PpuMI PstI PvuII SacI SanDI SbfI SexAI SfcI StyI TaqII TatI Tth111I Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI