Restriction map for
pFN21AE3875
(Linear) (Six-base) MAPSORT of: KIEE3875.seq Check: 9924 from: 1 to: 1056
KIEE3875.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
AccI GT'mk_AC
Cuts at: 0 785 919 998 1056
Size: 785 134 79 58
Fragments arranged by size:
785 134 79 58
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 502 916 1056
Size: 340 162 414 140
Fragments arranged by size:
414 340 162 140
AfeI AGC'GCT
Cuts at: 0 11 1056
Size: 11 1045
AflIII A'CryG_T
Cuts at: 0 260 465 1056
Size: 260 205 591
Fragments arranged by size:
591 260 205
AlwNI CAG_nnn'CTG
Cuts at: 0 41 1056
Size: 41 1015
ApoI r'AATT_y
Cuts at: 0 706 949 1056
Size: 706 243 107
Fragments arranged by size:
706 243 107
BamHI G'GATC_C
Cuts at: 0 70 1056
Size: 70 986
BanI G'GyrC_C
Cuts at: 0 637 1056
Size: 637 419
BanII G_rGCy'C
Cuts at: 0 212 353 563 1056
Size: 212 141 210 493
Fragments arranged by size:
493 212 210 141
BbsI GAAGACnn'nnnn_
Cuts at: 0 490 1056
Size: 490 566
BglI GCCn_nnn'nGGC
Cuts at: 0 460 1056
Size: 460 596
Bme1580I G_kGCm'C
Cuts at: 0 371 1056
Size: 371 685
BmgBI CAC'GTC
Cuts at: 0 468 1056
Size: 468 588
BmrI ACTGGGnnnn_n'
Cuts at: 0 109 1056
Size: 109 947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1056
Size: 226 830
BsaAI yAC'GTr
Cuts at: 0 263 366 1056
Size: 263 103 690
Fragments arranged by size:
690 263 103
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 601 631 1056
Size: 601 30 425
Fragments arranged by size:
601 425 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 727 769 1056
Size: 727 42 287
Fragments arranged by size:
727 287 42
BseYI C'CCAG_C
Cuts at: 0 746 1056
Size: 746 310
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 366 1056
Size: 214 152 690
Fragments arranged by size:
690 214 152
BsiHKAI G_wGCw'C
Cuts at: 0 212 353 977 1056
Size: 212 141 624 79
Fragments arranged by size:
624 212 141 79
BsmBI CGTCTCn'nnnn_
Cuts at: 0 474 1056
Size: 474 582
Bsp1286I G_dGCh'C
Cuts at: 0 212 353 371 563 977 1056
Size: 212 141 18 192 414 79
Fragments arranged by size:
414 212 192 141 79 18
BspHI T'CATG_A
Cuts at: 0 401 1056
Size: 401 655
BssSI C'ACGA_G
Cuts at: 0 346 823 1056
Size: 346 477 233
Fragments arranged by size:
477 346 233
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 1056
Size: 237 819
BstXI CCAn_nnnn'nTGG
Cuts at: 0 66 1056
Size: 66 990
BstYI r'GATC_y
Cuts at: 0 70 446 1056
Size: 70 376 610
Fragments arranged by size:
610 376 70
BtgI C'CryG_G
Cuts at: 0 440 1056
Size: 440 616
BtsI GCAGTG_nn'
Cuts at: 0 218 1056
Size: 218 838
DraIII CAC_nnn'GTG
Cuts at: 0 330 1056
Size: 330 726
EaeI y'GGCC_r
Cuts at: 0 56 452 1056
Size: 56 396 604
Fragments arranged by size:
604 396 56
EarI CTCTTCn'nnn_
Cuts at: 0 842 1056
Size: 842 214
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 462 570 1056
Size: 462 108 486
Fragments arranged by size:
486 462 108
EcoICRI GAG'CTC
Cuts at: 0 210 351 1056
Size: 210 141 705
Fragments arranged by size:
705 210 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 340 502 916 1056
Size: 226 114 162 414 140
Fragments arranged by size:
414 226 162 140 114
EcoNI CCTnn'n_nnAGG
Cuts at: 0 567 1056
Size: 567 489
EcoO109I rG'GnC_Cy
Cuts at: 0 164 681 1056
Size: 164 517 375
Fragments arranged by size:
517 375 164
EcoRI G'AATT_C
Cuts at: 0 949 1056
Size: 949 107
FspI TGC'GCA
Cuts at: 0 688 1056
Size: 688 368
HaeII r_GCGC'y
Cuts at: 0 13 281 1056
Size: 13 268 775
Fragments arranged by size:
775 268 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 1056
Size: 62 32 962
Fragments arranged by size:
962 62 32
MscI TGG'CCA
Cuts at: 0 58 1056
Size: 58 998
MspA1I CmG'CkG
Cuts at: 0 204 660 750 873 987 1056
Size: 204 456 90 123 114 69
Fragments arranged by size:
456 204 123 114 90 69
NcoI C'CATG_G
Cuts at: 0 440 1056
Size: 440 616
PfoI T'CCnGG_A
Cuts at: 0 554 1056
Size: 554 502
PmlI CAC'GTG
Cuts at: 0 263 1056
Size: 263 793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 952 984 1056
Size: 952 32 72
Fragments arranged by size:
952 72 32
PpuMI rG'GwC_Cy
Cuts at: 0 164 1056
Size: 164 892
PstI C_TGCA'G
Cuts at: 0 182 872 1056
Size: 182 690 184
Fragments arranged by size:
690 184 182
PvuII CAG'CTG
Cuts at: 0 204 873 987 1056
Size: 204 669 114 69
Fragments arranged by size:
669 204 114 69
SacI G_AGCT'C
Cuts at: 0 212 353 1056
Size: 212 141 703
Fragments arranged by size:
703 212 141
SanDI GG'GwC_CC
Cuts at: 0 164 1056
Size: 164 892
SbfI CC_TGCA'GG
Cuts at: 0 182 1056
Size: 182 874
SexAI A'CCwGG_T
Cuts at: 0 248 1056
Size: 248 808
SfcI C'TryA_G
Cuts at: 0 178 868 1056
Size: 178 690 188
Fragments arranged by size:
690 188 178
StyI C'CwwG_G
Cuts at: 0 371 440 1056
Size: 371 69 616
Fragments arranged by size:
616 371 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 128 616 688 760 1056
Size: 128 488 72 72 296
Fragments arranged by size:
488 296 128 72 72
TatI w'GTAC_w
Cuts at: 0 428 497 608 1056
Size: 428 69 111 448
Fragments arranged by size:
448 428 111 69
Tth111I GACn'n_nGTC
Cuts at: 0 700 1056
Size: 700 356
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI ApoI BamHI BanI
BanII BbsI BglI Bme1580I BmgBI BmrI BpmI BsaAI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BssSI BstAPI BstXI BstYI BtgI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I MscI MspA1I NcoI PfoI PmlI PpiI
PpuMI PstI PvuII SacI SanDI SbfI SexAI SfcI
StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SapI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI