Restriction map for
pFN21AE3874
(Linear) (Six-base) MAPSORT of: KIEE3874.seq Check: 3887 from: 1 to: 2097
KIEE3874.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 114 2097
Size: 114 1983
AccI GT'mk_AC
Cuts at: 0 1898 2097
Size: 1898 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1202 2097
Size: 776 426 895
Fragments arranged by size:
895 776 426
AgeI A'CCGG_T
Cuts at: 0 1607 2097
Size: 1607 490
AleI CACnn'nnGTG
Cuts at: 0 518 2097
Size: 518 1579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 685 1228 2097
Size: 685 543 869
Fragments arranged by size:
869 685 543
AlwNI CAG_nnn'CTG
Cuts at: 0 799 2097
Size: 799 1298
ApaI G_GGCC'C
Cuts at: 0 137 692 2097
Size: 137 555 1405
Fragments arranged by size:
1405 555 137
ApoI r'AATT_y
Cuts at: 0 309 775 1532 1887 2097
Size: 309 466 757 355 210
Fragments arranged by size:
757 466 355 309 210
AvaI C'yCGr_G
Cuts at: 0 203 209 439 533 1406 2097
Size: 203 6 230 94 873 691
Fragments arranged by size:
873 691 230 203 94 6
AvrII C'CTAG_G
Cuts at: 0 1378 2097
Size: 1378 719
BanI G'GyrC_C
Cuts at: 0 1108 2097
Size: 1108 989
BanII G_rGCy'C
Cuts at: 0 137 210 692 699 1966 2097
Size: 137 73 482 7 1267 131
Fragments arranged by size:
1267 482 137 131 73 7
BbeI G_GCGC'C
Cuts at: 0 1112 2097
Size: 1112 985
BbsI GAAGACnn'nnnn_
Cuts at: 0 1797 2049 2097
Size: 1797 252 48
Fragments arranged by size:
1797 252 48
BclI T'GATC_A
Cuts at: 0 1515 2097
Size: 1515 582
Bme1580I G_kGCm'C
Cuts at: 0 137 692 1207 2097
Size: 137 555 515 890
Fragments arranged by size:
890 555 515 137
BmgBI CAC'GTC
Cuts at: 0 630 2097
Size: 630 1467
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 354 2097
Size: 354 1743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 874 1933 2097
Size: 64 810 1059 164
Fragments arranged by size:
1059 810 164 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 151 231 2097
Size: 151 80 1866
Fragments arranged by size:
1866 151 80
BsaAI yAC'GTr
Cuts at: 0 893 2097
Size: 893 1204
BsaHI Gr'CG_yC
Cuts at: 0 1109 1761 2097
Size: 1109 652 336
Fragments arranged by size:
1109 652 336
BsaWI w'CCGG_w
Cuts at: 0 563 1607 2097
Size: 563 1044 490
Fragments arranged by size:
1044 563 490
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 403 2097
Size: 400 3 1694
Fragments arranged by size:
1694 400 3
BseYI C'CCAG_C
Cuts at: 0 276 2097
Size: 276 1821
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 2092 2097
Size: 798 1294 5
Fragments arranged by size:
1294 798 5
BsiEI CG_ry'CG
Cuts at: 0 141 187 1664 2097
Size: 141 46 1477 433
Fragments arranged by size:
1477 433 141 46
BsiHKAI G_wGCw'C
Cuts at: 0 410 614 699 1652 2097
Size: 410 204 85 953 445
Fragments arranged by size:
953 445 410 204 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1651 2097
Size: 1651 446
Bsp1286I G_dGCh'C
Cuts at: 0 137 210 410 614 692 699 1207 1652
Size: 137 73 200 204 78 7 508 445
Cuts at: 1652 1966 2097
Size: 314 131
Fragments arranged by size:
508 445 314 204 200 137 131 78
73 7
BspHI T'CATG_A
Cuts at: 0 327 602 1518 2097
Size: 327 275 916 579
Fragments arranged by size:
916 579 327 275
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 260 1152 1407 2097
Size: 114 146 892 255 690
Fragments arranged by size:
892 690 255 146 114
BsrBI CCG'CTC
Cuts at: 0 306 1154 2097
Size: 306 848 943
Fragments arranged by size:
943 848 306
BsrFI r'CCGG_y
Cuts at: 0 160 281 1607 2097
Size: 160 121 1326 490
Fragments arranged by size:
1326 490 160 121
BstAPI GCAn_nnn'nTGC
Cuts at: 0 230 1441 2097
Size: 230 1211 656
Fragments arranged by size:
1211 656 230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1423 2097
Size: 1423 674
BstYI r'GATC_y
Cuts at: 0 1327 2097
Size: 1327 770
Bsu36I CC'TnA_GG
Cuts at: 0 927 2097
Size: 927 1170
BtgI C'CryG_G
Cuts at: 0 48 2097
Size: 48 2049
BtsI GCAGTG_nn'
Cuts at: 0 410 2097
Size: 410 1687
EaeI y'GGCC_r
Cuts at: 0 128 194 596 1304 2097
Size: 128 66 402 708 793
Fragments arranged by size:
793 708 402 128 66
EarI CTCTTCn'nnn_
Cuts at: 0 338 1072 1161 1862 1908 2077 2097
Size: 338 734 89 701 46 169 20
Fragments arranged by size:
734 701 338 169 89 46 20
EcoICRI GAG'CTC
Cuts at: 0 697 2097
Size: 697 1400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 776 874 1202 1933 2097
Size: 64 712 98 328 731 164
Fragments arranged by size:
731 712 328 164 98 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 926 1935 2097
Size: 926 1009 162
Fragments arranged by size:
1009 926 162
EcoO109I rG'GnC_Cy
Cuts at: 0 688 809 1011 2097
Size: 688 121 202 1086
Fragments arranged by size:
1086 688 202 121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 443 796 2097
Size: 443 353 1301
Fragments arranged by size:
1301 443 353
HaeII r_GCGC'y
Cuts at: 0 1112 2097
Size: 1112 985
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 197 229 500 532 735 767 894 926
Size: 197 32 271 32 203 32 127 32
Cuts at: 926 1462 1494 1507 1539 2097
Size: 536 32 13 32 558
Fragments arranged by size:
558 536 271 203 197 127 32 32
32 32 32 32 13
HincII GTy'rAC
Cuts at: 0 1467 1899 2097
Size: 1467 432 198
Fragments arranged by size:
1467 432 198
HpaI GTT'AAC
Cuts at: 0 1467 2097
Size: 1467 630
KasI G'GCGC_C
Cuts at: 0 1108 2097
Size: 1108 989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 1782 2097
Size: 134 1648 315
Fragments arranged by size:
1648 315 134
MscI TGG'CCA
Cuts at: 0 196 598 1306 2097
Size: 196 402 708 791
Fragments arranged by size:
791 708 402 196
MslI CAynn'nnrTG
Cuts at: 0 518 2097
Size: 518 1579
MspA1I CmG'CkG
Cuts at: 0 1355 2097
Size: 1355 742
NarI GG'CG_CC
Cuts at: 0 1109 2097
Size: 1109 988
NspI r_CATG'y
Cuts at: 0 672 2097
Size: 672 1425
PasI CC'CwG_GG
Cuts at: 0 685 2097
Size: 685 1412
PflMI CCAn_nnn'nTGG
Cuts at: 0 1817 2097
Size: 1817 280
PfoI T'CCnGG_A
Cuts at: 0 80 644 1090 2097
Size: 80 564 446 1007
Fragments arranged by size:
1007 564 446 80
PpuMI rG'GwC_Cy
Cuts at: 0 809 1011 2097
Size: 809 202 1086
Fragments arranged by size:
1086 809 202
PsiI TTA'TAA
Cuts at: 0 458 2097
Size: 458 1639
PspOMI G'GGCC_C
Cuts at: 0 133 688 2097
Size: 133 555 1409
Fragments arranged by size:
1409 555 133
PspXI vC'TCGA_Gb
Cuts at: 0 203 2097
Size: 203 1894
PstI C_TGCA'G
Cuts at: 0 125 1582 2097
Size: 125 1457 515
Fragments arranged by size:
1457 515 125
PvuI CG_AT'CG
Cuts at: 0 187 1664 2097
Size: 187 1477 433
Fragments arranged by size:
1477 433 187
SacI G_AGCT'C
Cuts at: 0 699 2097
Size: 699 1398
SalI G'TCGA_C
Cuts at: 0 1897 2097
Size: 1897 200
SapI GCTCTTCn'nnn_
Cuts at: 0 1072 1161 2097
Size: 1072 89 936
Fragments arranged by size:
1072 936 89
ScaI AGT'ACT
Cuts at: 0 229 2097
Size: 229 1868
SfcI C'TryA_G
Cuts at: 0 121 1227 1578 1703 2097
Size: 121 1106 351 125 394
Fragments arranged by size:
1106 394 351 125 121
SfoI GGC'GCC
Cuts at: 0 1110 2097
Size: 1110 987
SmaI CCC'GGG
Cuts at: 0 211 2097
Size: 211 1886
SmlI C'TyrA_G
Cuts at: 0 203 2097
Size: 203 1894
SspI AAT'ATT
Cuts at: 0 322 2097
Size: 322 1775
StyI C'CwwG_G
Cuts at: 0 938 1104 1378 1989 2097
Size: 938 166 274 611 108
Fragments arranged by size:
938 611 274 166 108
TatI w'GTAC_w
Cuts at: 0 227 2097
Size: 227 1870
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 807 832 839 864 2097
Size: 807 25 7 25 1233
Fragments arranged by size:
1233 807 25 25 7
Tth111I GACn'n_nGTC
Cuts at: 0 631 2097
Size: 631 1466
XhoI C'TCGA_G
Cuts at: 0 203 2097
Size: 203 1894
XmaI C'CCGG_G
Cuts at: 0 209 2097
Size: 209 1888
Enzymes that do cut:
AarI AccI AcuI AgeI AleI AlfI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbeI BbsI BclI
Bme1580I BmgBI BplI BpmI BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HpaI KasI MmeI MscI MslI
MspA1I NarI NspI PasI PflMI PfoI PpuMI PsiI
PspOMI PspXI PstI PvuI SacI SalI SapI ScaI
SfcI SfoI SmaI SmlI SspI StyI TatI TstI
Tth111I XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AloI
ApaLI AscI AseI AsiSI BaeI BamHI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmrI BmtI Bpu10I
BpuEI BsaBI BsaXI BsiWI BsmI BspEI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI
FspI FspAI HindIII KpnI MfeI MluI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsrI PvuII RsrII SacII
SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI TaqII XbaI XcmI XmnI
ZraI