Restriction map for
pFN21AE3873
(Linear) (Six-base) MAPSORT of: KIEE3873.seq Check: 3929 from: 1 to: 1452
KIEE3873.orf
With 182 enzymes: SgfI *
August 4, 2021 20:20 ..
AatII G_ACGT'C
Cuts at: 0 92 1452
Size: 92 1360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1452
Size: 1120 332
AfeI AGC'GCT
Cuts at: 0 718 1452
Size: 718 734
AflIII A'CryG_T
Cuts at: 0 498 1452
Size: 498 954
AlwNI CAG_nnn'CTG
Cuts at: 0 209 369 1093 1149 1152 1305 1452
Size: 209 160 724 56 3 153 147
Fragments arranged by size:
724 209 160 153 147 56 3
AvaI C'yCGr_G
Cuts at: 0 22 644 917 1128 1332 1452
Size: 22 622 273 211 204 120
Fragments arranged by size:
622 273 211 204 120 22
BanI G'GyrC_C
Cuts at: 0 519 1452
Size: 519 933
BanII G_rGCy'C
Cuts at: 0 247 1228 1452
Size: 247 981 224
Fragments arranged by size:
981 247 224
BglII A'GATC_T
Cuts at: 0 409 1452
Size: 409 1043
BlpI GC'TnA_GC
Cuts at: 0 143 1452
Size: 143 1309
Bme1580I G_kGCm'C
Cuts at: 0 23 120 235 524 985 1452
Size: 23 97 115 289 461 467
Fragments arranged by size:
467 461 289 115 97 23
BmgBI CAC'GTC
Cuts at: 0 516 1452
Size: 516 936
BmrI ACTGGGnnnn_n'
Cuts at: 0 229 1112 1452
Size: 229 883 340
Fragments arranged by size:
883 340 229
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 158 738 1191 1452
Size: 158 580 453 261
Fragments arranged by size:
580 453 261 158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 269 391 717 883 1045 1078 1294
Size: 118 151 122 326 166 162 33 216
Cuts at: 1294 1452
Size: 158
Fragments arranged by size:
326 216 166 162 158 151 122 118
33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1204 1452
Size: 1204 248
BsaAI yAC'GTr
Cuts at: 0 18 1452
Size: 18 1434
BsaBI GATnn'nnATC
Cuts at: 0 637 1452
Size: 637 815
BsaHI Gr'CG_yC
Cuts at: 0 89 1452
Size: 89 1363
BsaWI w'CCGG_w
Cuts at: 0 987 1409 1452
Size: 987 422 43
Fragments arranged by size:
987 422 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 1452
Size: 436 30 986
Fragments arranged by size:
986 436 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 241 711 1183 1291 1452
Size: 115 126 470 472 108 161
Fragments arranged by size:
472 470 161 126 115 108
BseYI C'CCAG_C
Cuts at: 0 355 362 828 1086 1152 1452
Size: 355 7 466 258 66 300
Fragments arranged by size:
466 355 300 258 66 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 769 1452
Size: 262 507 683
Fragments arranged by size:
683 507 262
BsiHKAI G_wGCw'C
Cuts at: 0 686 1043 1175 1228 1397 1452
Size: 686 357 132 53 169 55
Fragments arranged by size:
686 357 169 132 55 53
BsmBI CGTCTCn'nnnn_
Cuts at: 0 96 910 1452
Size: 96 814 542
Fragments arranged by size:
814 542 96
Bsp1286I G_dGCh'C
Cuts at: 0 23 120 235 247 524 686 985 1043
Size: 23 97 115 12 277 162 299 58
Cuts at: 1043 1175 1228 1397 1452
Size: 132 53 169 55
Fragments arranged by size:
299 277 169 162 132 115 97 58
55 53 23 12
BsrBI CCG'CTC
Cuts at: 0 731 1188 1452
Size: 731 457 264
Fragments arranged by size:
731 457 264
BsrDI GCAATG_nn'
Cuts at: 0 306 737 1452
Size: 306 431 715
Fragments arranged by size:
715 431 306
BsrFI r'CCGG_y
Cuts at: 0 1370 1427 1452
Size: 1370 57 25
Fragments arranged by size:
1370 57 25
BstXI CCAn_nnnn'nTGG
Cuts at: 0 247 1452
Size: 247 1205
BstYI r'GATC_y
Cuts at: 0 409 632 1246 1452
Size: 409 223 614 206
Fragments arranged by size:
614 409 223 206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 327 1452
Size: 327 1125
DrdI GACnn_nn'nnGTC
Cuts at: 0 88 1109 1452
Size: 88 1021 343
Fragments arranged by size:
1021 343 88
EaeI y'GGCC_r
Cuts at: 0 779 839 1452
Size: 779 60 613
Fragments arranged by size:
779 613 60
EarI CTCTTCn'nnn_
Cuts at: 0 604 1452
Size: 604 848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 449 508 1452
Size: 449 59 944
Fragments arranged by size:
944 449 59
EcoICRI GAG'CTC
Cuts at: 0 1226 1452
Size: 1226 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 269 391 717 883 1045 1078 1120
Size: 118 151 122 326 166 162 33 42
Cuts at: 1120 1294 1452
Size: 174 158
Fragments arranged by size:
326 174 166 162 158 151 122 118
42 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 1276 1452
Size: 100 1176 176
Fragments arranged by size:
1176 176 100
EcoO109I rG'GnC_Cy
Cuts at: 0 1111 1302 1452
Size: 1111 191 150
Fragments arranged by size:
1111 191 150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 615 1390 1452
Size: 615 775 62
Fragments arranged by size:
775 615 62
HaeII r_GCGC'y
Cuts at: 0 369 720 1452
Size: 369 351 732
Fragments arranged by size:
732 369 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 668 700 1452
Size: 668 32 752
Fragments arranged by size:
752 668 32
HincII GTy'rAC
Cuts at: 0 255 1452
Size: 255 1197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 785 1452
Size: 785 667
MscI TGG'CCA
Cuts at: 0 781 841 1452
Size: 781 60 611
Fragments arranged by size:
781 611 60
MslI CAynn'nnrTG
Cuts at: 0 580 1452
Size: 580 872
MspA1I CmG'CkG
Cuts at: 0 837 1023 1149 1452
Size: 837 186 126 303
Fragments arranged by size:
837 303 186 126
NdeI CA'TA_TG
Cuts at: 0 510 963 1452
Size: 510 453 489
Fragments arranged by size:
510 489 453
NspI r_CATG'y
Cuts at: 0 502 1452
Size: 502 950
PciI A'CATG_T
Cuts at: 0 498 1452
Size: 498 954
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 1093 1452
Size: 369 724 359
Fragments arranged by size:
724 369 359
PpuMI rG'GwC_Cy
Cuts at: 0 1111 1302 1452
Size: 1111 191 150
Fragments arranged by size:
1111 191 150
PshAI GACnn'nnGTC
Cuts at: 0 199 1452
Size: 199 1253
PstI C_TGCA'G
Cuts at: 0 794 1452
Size: 794 658
PvuII CAG'CTG
Cuts at: 0 837 1023 1149 1452
Size: 837 186 126 303
Fragments arranged by size:
837 303 186 126
SacI G_AGCT'C
Cuts at: 0 1228 1452
Size: 1228 224
SfcI C'TryA_G
Cuts at: 0 790 1452
Size: 790 662
SmaI CCC'GGG
Cuts at: 0 24 919 1130 1452
Size: 24 895 211 322
Fragments arranged by size:
895 322 211 24
SmlI C'TyrA_G
Cuts at: 0 1183 1452
Size: 1183 269
StyI C'CwwG_G
Cuts at: 0 1432 1439 1452
Size: 1432 7 13
Fragments arranged by size:
1432 13 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 842 1100 1452
Size: 842 258 352
Fragments arranged by size:
842 352 258
TatI w'GTAC_w
Cuts at: 0 293 1452
Size: 293 1159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 1452
Size: 214 32 1206
Fragments arranged by size:
1206 214 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 534 849 1090 1452
Size: 534 315 241 362
Fragments arranged by size:
534 362 315 241
XmaI C'CCGG_G
Cuts at: 0 22 917 1128 1452
Size: 22 895 211 324
Fragments arranged by size:
895 324 211 22
XmnI GAAnn'nnTTC
Cuts at: 0 500 1452
Size: 500 952
ZraI GAC'GTC
Cuts at: 0 90 1452
Size: 90 1362
Enzymes that do cut:
AatII AcuI AfeI AflIII AlwNI AvaI BanI BanII
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BstXI BstYI
BtgZI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NdeI NspI PciI PflMI PpuMI PshAI PstI
PvuII SacI SfcI SmaI SmlI StyI TaqII TatI
TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BmtI Bpu10I BsaI BsiEI BsiWI
BsmI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII EagI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI