Restriction map for
pFN21AE3830
(Linear) (Six-base) MAPSORT of: KIEE3830.seq Check: 594 from: 1 to: 8892
KIEE3830.orf
With 182 enzymes: SgfI *
March 22, 2012 16:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 760 8892
Size: 760 8132
AccI GT'mk_AC
Cuts at: 0 324 5553 6052 6227 6701 7599 7937 8892
Size: 324 5229 499 175 474 898 338 955
Fragments arranged by size:
5229 955 898 499 474 338 324 175
AclI AA'CG_TT
Cuts at: 0 1835 2231 8892
Size: 1835 396 6661
Fragments arranged by size:
6661 1835 396
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 1985 2809 4667 5557 6970 8892
Size: 497 1488 824 1858 890 1413 1922
Fragments arranged by size:
1922 1858 1488 1413 890 824 497
AfeI AGC'GCT
Cuts at: 0 4546 8521 8892
Size: 4546 3975 371
Fragments arranged by size:
4546 3975 371
AflII C'TTAA_G
Cuts at: 0 1306 8892
Size: 1306 7586
AflIII A'CryG_T
Cuts at: 0 4610 8892
Size: 4610 4282
AhdI GACnn_n'nnGTC
Cuts at: 0 2457 8892
Size: 2457 6435
AleI CACnn'nnGTG
Cuts at: 0 3521 7136 8892
Size: 3521 3615 1756
Fragments arranged by size:
3615 3521 1756
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 8863 8892
Size: 8863 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4652 4684 8892
Size: 4652 32 4208
Fragments arranged by size:
4652 4208 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2062 7702 8892
Size: 2062 5640 1190
Fragments arranged by size:
5640 2062 1190
ApoI r'AATT_y
Cuts at: 0 249 1781 3504 3646 3899 6212 7337 8892
Size: 249 1532 1723 142 253 2313 1125 1555
Fragments arranged by size:
2313 1723 1555 1532 1125 253 249 142
AseI AT'TA_AT
Cuts at: 0 566 950 1463 2932 8892
Size: 566 384 513 1469 5960
Fragments arranged by size:
5960 1469 566 513 384
AvaI C'yCGr_G
Cuts at: 0 3935 7304 8892
Size: 3935 3369 1588
Fragments arranged by size:
3935 3369 1588
AvrII C'CTAG_G
Cuts at: 0 6507 8892
Size: 6507 2385
BamHI G'GATC_C
Cuts at: 0 600 2829 3849 8892
Size: 600 2229 1020 5043
Fragments arranged by size:
5043 2229 1020 600
BanI G'GyrC_C
Cuts at: 0 4451 8214 8892
Size: 4451 3763 678
Fragments arranged by size:
4451 3763 678
BanII G_rGCy'C
Cuts at: 0 3358 5186 6476 8892
Size: 3358 1828 1290 2416
Fragments arranged by size:
3358 2416 1828 1290
BbsI GAAGACnn'nnnn_
Cuts at: 0 484 686 2974 3323 5051 7209 8892
Size: 484 202 2288 349 1728 2158 1683
Fragments arranged by size:
2288 2158 1728 1683 484 349 202
BbvCI CC'TCA_GC
Cuts at: 0 3778 6440 8892
Size: 3778 2662 2452
Fragments arranged by size:
3778 2662 2452
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4003 4037 7231 7265 8892
Size: 4003 34 3194 34 1627
Fragments arranged by size:
4003 3194 1627 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4220 6389 8892
Size: 4220 2169 2503
Fragments arranged by size:
4220 2503 2169
BclI T'GATC_A
Cuts at: 0 758 1247 8418 8892
Size: 758 489 7171 474
Fragments arranged by size:
7171 758 489 474
BfrBI ATG'CAT
Cuts at: 0 1499 4281 8892
Size: 1499 2782 4611
Fragments arranged by size:
4611 2782 1499
BglI GCCn_nnn'nGGC
Cuts at: 0 2682 6371 8892
Size: 2682 3689 2521
Fragments arranged by size:
3689 2682 2521
BglII A'GATC_T
Cuts at: 0 3939 7325 7442 7458 8762 8892
Size: 3939 3386 117 16 1304 130
Fragments arranged by size:
3939 3386 1304 130 117 16
BlpI GC'TnA_GC
Cuts at: 0 1000 3721 8892
Size: 1000 2721 5171
Fragments arranged by size:
5171 2721 1000
BmgBI CAC'GTC
Cuts at: 0 5947 8892
Size: 5947 2945
BmrI ACTGGGnnnn_n'
Cuts at: 0 931 2059 3383 3587 4394 5849 6977 7104
Size: 931 1128 1324 204 807 1455 1128 127
Cuts at: 7104 7221 7570 8700 8892
Size: 117 349 1130 192
Fragments arranged by size:
1455 1324 1130 1128 1128 931 807 349
204 192 127 117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6985 7461 8892
Size: 6985 476 1431
Fragments arranged by size:
6985 1431 476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 521 2415 3402 3892 4845 6041 6067
Size: 256 265 1894 987 490 953 1196 26
Cuts at: 6067 8647 8845 8892
Size: 2580 198 47
Fragments arranged by size:
2580 1894 1196 987 953 490 265 256
198 47 26
Bpu10I CC'TnA_GC
Cuts at: 0 3778 6440 8892
Size: 3778 2662 2452
Fragments arranged by size:
3778 2662 2452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2905 3238 3540 8892
Size: 2905 333 302 5352
Fragments arranged by size:
5352 2905 333 302
BsaI GGTCTCn'nnnn_
Cuts at: 0 413 2192 3577 4047 7684 7780 8289 8892
Size: 413 1779 1385 470 3637 96 509 603
Fragments arranged by size:
3637 1779 1385 603 509 470 413 96
BsaAI yAC'GTr
Cuts at: 0 8282 8892
Size: 8282 610
BsaWI w'CCGG_w
Cuts at: 0 1587 6704 8378 8892
Size: 1587 5117 1674 514
Fragments arranged by size:
5117 1674 1587 514
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 666 696 1980 2010 4164 4194 4843 4873
Size: 666 30 1284 30 2154 30 649 30
Cuts at: 4873 8892
Size: 4019
Fragments arranged by size:
4019 2154 1284 666 649 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3476 3486 3681 4223 8644 8892
Size: 3476 10 195 542 4421 248
Fragments arranged by size:
4421 3476 542 248 195 10
BseYI C'CCAG_C
Cuts at: 0 178 3241 3830 4988 5012 5871 6484 6739
Size: 178 3063 589 1158 24 859 613 255
Cuts at: 6739 8620 8892
Size: 1881 272
Fragments arranged by size:
3063 1881 1158 859 613 589 272 255
178 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1664 2075 4889 5210 5641 8382 8892
Size: 1664 411 2814 321 431 2741 510
Fragments arranged by size:
2814 2741 1664 510 431 411 321
BsiHKAI G_wGCw'C
Cuts at: 0 4442 5032 7850 8892
Size: 4442 590 2818 1042
Fragments arranged by size:
4442 2818 1042 590
BsmI GAATG_Cn'
Cuts at: 0 472 1499 1982 3672 5596 7428 7551 8892
Size: 472 1027 483 1690 1924 1832 123 1341
Fragments arranged by size:
1924 1832 1690 1341 1027 483 472 123
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5630 8892
Size: 5630 3262
Bsp1286I G_dGCh'C
Cuts at: 0 3358 4442 5032 5186 6476 7850 8892
Size: 3358 1084 590 154 1290 1374 1042
Fragments arranged by size:
3358 1374 1290 1084 1042 590 154
BspEI T'CCGG_A
Cuts at: 0 1587 8892
Size: 1587 7305
BspHI T'CATG_A
Cuts at: 0 2313 3768 7220 7778 8892
Size: 2313 1455 3452 558 1114
Fragments arranged by size:
3452 2313 1455 1114 558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 336 760 1231 3364 8266 8892
Size: 336 424 471 2133 4902 626
Fragments arranged by size:
4902 2133 626 471 424 336
BsrDI GCAATG_nn'
Cuts at: 0 1193 3455 4082 6385 6424 8892
Size: 1193 2262 627 2303 39 2468
Fragments arranged by size:
2468 2303 2262 1193 627 39
BsrFI r'CCGG_y
Cuts at: 0 2183 8892
Size: 2183 6709
BsrGI T'GTAC_A
Cuts at: 0 434 8324 8892
Size: 434 7890 568
Fragments arranged by size:
7890 568 434
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2062 5910 8892
Size: 2062 3848 2982
Fragments arranged by size:
3848 2982 2062
BstBI TT'CG_AA
Cuts at: 0 3837 3960 4023 7260 8886 8892
Size: 3837 123 63 3237 1626 6
Fragments arranged by size:
3837 3237 1626 123 63 6
BstEII G'GTnAC_C
Cuts at: 0 2693 8140 8892
Size: 2693 5447 752
Fragments arranged by size:
5447 2693 752
BstYI r'GATC_y
Cuts at: 0 600 855 1534 2829 3849 3939 7181 7325
Size: 600 255 679 1295 1020 90 3242 144
Cuts at: 7325 7442 7458 7481 8372 8762 8892
Size: 117 16 23 891 390 130
Fragments arranged by size:
3242 1295 1020 891 679 600 390 255
144 130 117 90 23 16
BstZ17I GTA'TAC
Cuts at: 0 6053 6702 8892
Size: 6053 649 2190
Fragments arranged by size:
6053 2190 649
Bsu36I CC'TnA_GG
Cuts at: 0 8723 8892
Size: 8723 169
BtgI C'CryG_G
Cuts at: 0 5186 6890 8892
Size: 5186 1704 2002
Fragments arranged by size:
5186 2002 1704
BtsI GCAGTG_nn'
Cuts at: 0 1282 2076 2802 4803 4945 5653 6166 7692
Size: 1282 794 726 2001 142 708 513 1526
Cuts at: 7692 7870 8651 8892
Size: 178 781 241
Fragments arranged by size:
2001 1526 1282 794 781 726 708 513
241 178 142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 7862 7897 8892
Size: 7862 35 995
Fragments arranged by size:
7862 995 35
DraI TTT'AAA
Cuts at: 0 254 611 1596 2045 2226 2540 2975 8892
Size: 254 357 985 449 181 314 435 5917
Fragments arranged by size:
5917 985 449 435 357 314 254 181
EaeI y'GGCC_r
Cuts at: 0 1148 4866 5017 5450 6893 8025 8892
Size: 1148 3718 151 433 1443 1132 867
Fragments arranged by size:
3718 1443 1148 1132 867 433 151
EarI CTCTTCn'nnn_
Cuts at: 0 131 590 1947 3144 4521 5737 5801 6922
Size: 131 459 1357 1197 1377 1216 64 1121
Cuts at: 6922 8573 8892
Size: 1651 319
Fragments arranged by size:
1651 1377 1357 1216 1197 1121 459 319
131 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 497 521 1985 2415 2809 3402 3892
Size: 256 241 24 1464 430 394 593 490
Cuts at: 3892 4667 4845 5557 6041 6067 6970 8647 8845
Size: 775 178 712 484 26 903 1677 198
Cuts at: 8845 8892
Size: 47
Fragments arranged by size:
1677 1464 903 775 712 593 490 484
430 394 256 241 198 178 47 26
24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1238 6511 7762 8892
Size: 1238 5273 1251 1130
Fragments arranged by size:
5273 1251 1238 1130
EcoO109I rG'GnC_Cy
Cuts at: 0 141 8255 8892
Size: 141 8114 637
Fragments arranged by size:
8114 637 141
EcoRV GAT'ATC
Cuts at: 0 6150 8892
Size: 6150 2742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 160 1985 5336 6828 8876 8892
Size: 160 1825 3351 1492 2048 16
Fragments arranged by size:
3351 2048 1825 1492 160 16
HaeII r_GCGC'y
Cuts at: 0 4548 6091 8523 8892
Size: 4548 1543 2432 369
Fragments arranged by size:
4548 2432 1543 369
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1501 1533 2443 2475 2802 2834 3138 3170
Size: 1501 32 910 32 327 32 304 32
Cuts at: 3170 3426 3458 3897 3929 4498 4530 4993 5025
Size: 256 32 439 32 569 32 463 32
Cuts at: 5025 5697 5729 5841 5873 6818 6850 6951 6983
Size: 672 32 112 32 945 32 101 32
Cuts at: 6983 7434 7466 7992 8024 8818 8850 8892
Size: 451 32 526 32 794 32 42
Fragments arranged by size:
1501 945 910 794 672 569 526 463
451 439 327 304 256 112 101 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 325 2010 5357 5554 5619 8711 8892
Size: 325 1685 3347 197 65 3092 181
Fragments arranged by size:
3347 3092 1685 325 197 181 65
HindIII A'AGCT_T
Cuts at: 0 4641 4752 5826 7511 8101 8892
Size: 4641 111 1074 1685 590 791
Fragments arranged by size:
4641 1685 1074 791 590 111
HpaI GTT'AAC
Cuts at: 0 2010 8892
Size: 2010 6882
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 1074 1776 4408 4551 6081 7153 8249
Size: 819 255 702 2632 143 1530 1072 1096
Cuts at: 8249 8892
Size: 643
Fragments arranged by size:
2632 1530 1096 1072 819 702 643 255
143
MscI TGG'CCA
Cuts at: 0 1150 5452 6895 8027 8892
Size: 1150 4302 1443 1132 865
Fragments arranged by size:
4302 1443 1150 1132 865
MslI CAynn'nnrTG
Cuts at: 0 3521 3578 6071 6656 7136 8809 8892
Size: 3521 57 2493 585 480 1673 83
Fragments arranged by size:
3521 2493 1673 585 480 83 57
MspA1I CmG'CkG
Cuts at: 0 666 940 3241 4177 4838 5016 5157 5366
Size: 666 274 2301 936 661 178 141 209
Cuts at: 5366 6739 7279 8892
Size: 1373 540 1613
Fragments arranged by size:
2301 1613 1373 936 666 661 540 274
209 178 141
NcoI C'CATG_G
Cuts at: 0 5186 6890 8892
Size: 5186 1704 2002
Fragments arranged by size:
5186 2002 1704
NsiI A_TGCA'T
Cuts at: 0 1501 4283 8892
Size: 1501 2782 4609
Fragments arranged by size:
4609 2782 1501
NspI r_CATG'y
Cuts at: 0 1499 2183 3688 4285 4614 6735 7530 8892
Size: 1499 684 1505 597 329 2121 795 1362
Fragments arranged by size:
2121 1505 1499 1362 795 684 597 329
PasI CC'CwG_GG
Cuts at: 0 5274 8892
Size: 5274 3618
PciI A'CATG_T
Cuts at: 0 4610 8892
Size: 4610 4282
PflMI CCAn_nnn'nTGG
Cuts at: 0 4144 6891 8892
Size: 4144 2747 2001
Fragments arranged by size:
4144 2747 2001
PfoI T'CCnGG_A
Cuts at: 0 4529 6057 8506 8892
Size: 4529 1528 2449 386
Fragments arranged by size:
4529 2449 1528 386
PpuMI rG'GwC_Cy
Cuts at: 0 141 8255 8892
Size: 141 8114 637
Fragments arranged by size:
8114 637 141
PshAI GACnn'nnGTC
Cuts at: 0 7696 8892
Size: 7696 1196
PsiI TTA'TAA
Cuts at: 0 2471 6764 7361 8892
Size: 2471 4293 597 1531
Fragments arranged by size:
4293 2471 1531 597
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 714 746 918 950 969 1001 5078 5110
Size: 714 32 172 32 19 32 4077 32
Cuts at: 5110 8892
Size: 3782
Fragments arranged by size:
4077 3782 714 172 32 32 32 32
19
PstI C_TGCA'G
Cuts at: 0 4843 5156 5307 7244 7284 8892
Size: 4843 313 151 1937 40 1608
Fragments arranged by size:
4843 1937 1608 313 151 40
PvuII CAG'CTG
Cuts at: 0 666 3241 4177 4838 5366 6739 7279 8892
Size: 666 2575 936 661 528 1373 540 1613
Fragments arranged by size:
2575 1613 1373 936 666 661 540 528
RsrII CG'GwC_CG
Cuts at: 0 8034 8892
Size: 8034 858
SalI G'TCGA_C
Cuts at: 0 323 5552 8892
Size: 323 5229 3340
Fragments arranged by size:
5229 3340 323
SapI GCTCTTCn'nnn_
Cuts at: 0 1947 4521 5801 8892
Size: 1947 2574 1280 3091
Fragments arranged by size:
3091 2574 1947 1280
ScaI AGT'ACT
Cuts at: 0 81 2103 4125 5073 7558 8892
Size: 81 2022 2022 948 2485 1334
Fragments arranged by size:
2485 2022 2022 1334 948 81
SfcI C'TryA_G
Cuts at: 0 731 782 935 986 1763 2868 3014 3713
Size: 731 51 153 51 777 1105 146 699
Cuts at: 3713 4548 4839 5152 5303 6002 7240 7280 8246
Size: 835 291 313 151 699 1238 40 966
Cuts at: 8246 8892
Size: 646
Fragments arranged by size:
1238 1105 966 835 777 731 699 699
646 313 291 153 151 146 51 51
40
SmlI C'TyrA_G
Cuts at: 0 1306 2884 3217 3519 8892
Size: 1306 1578 333 302 5373
Fragments arranged by size:
5373 1578 1306 333 302
SnaBI TAC'GTA
Cuts at: 0 8282 8892
Size: 8282 610
SpeI A'CTAG_T
Cuts at: 0 640 2017 2104 3340 8892
Size: 640 1377 87 1236 5552
Fragments arranged by size:
5552 1377 1236 640 87
SphI G_CATG'C
Cuts at: 0 4285 8892
Size: 4285 4607
SspI AAT'ATT
Cuts at: 0 7146 7293 8892
Size: 7146 147 1599
Fragments arranged by size:
7146 1599 147
StuI AGG'CCT
Cuts at: 0 2490 2676 3460 4351 7609 7878 8892
Size: 2490 186 784 891 3258 269 1014
Fragments arranged by size:
3258 2490 1014 891 784 269 186
StyI C'CwwG_G
Cuts at: 0 355 1151 1947 4703 4761 5186 5926 6507
Size: 355 796 796 2756 58 425 740 581
Cuts at: 6507 6890 7873 8237 8892
Size: 383 983 364 655
Fragments arranged by size:
2756 983 796 796 740 655 581 425
383 364 355 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 368 2041 5282 6402 8892
Size: 368 1673 3241 1120 2490
Fragments arranged by size:
3241 2490 1673 1120 368
TatI w'GTAC_w
Cuts at: 0 79 434 540 1321 2101 2718 4123 4968
Size: 79 355 106 781 780 617 1405 845
Cuts at: 4968 5071 7556 8324 8892
Size: 103 2485 768 568
Fragments arranged by size:
2485 1405 845 781 780 768 617 568
355 106 103 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 756 788 2410 2442 2736 2768 4209 4241
Size: 756 32 1622 32 294 32 1441 32
Cuts at: 4241 4245 4277 6294 6326 8164 8196 8247 8279
Size: 4 32 2017 32 1838 32 51 32
Cuts at: 8279 8892
Size: 613
Fragments arranged by size:
2017 1838 1622 1441 756 613 294 51
32 32 32 32 32 32 32 32
4
Tth111I GACn'n_nGTC
Cuts at: 0 5473 8892
Size: 5473 3419
XbaI T'CTAG_A
Cuts at: 0 6423 8892
Size: 6423 2469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1834 8892
Size: 1834 7058
XmnI GAAnn'nnTTC
Cuts at: 0 6431 8207 8582 8892
Size: 6431 1776 375 310
Fragments arranged by size:
6431 1776 375 310
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApoI AseI AvaI AvrII
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtsI CspCI DraI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
HpaI MmeI MscI MslI MspA1I NcoI NsiI NspI
PasI PciI PflMI PfoI PpuMI PshAI PsiI PsrI
PstI PvuII RsrII SalI SapI ScaI SfcI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AgeI ApaI ApaLI AscI AsiSI BaeI
BbeI Bme1580I BmtI BsaBI BsaHI BsiEI BsiWI BsrBI
BssHII BssSI BstXI BtgZI ClaI DraIII DrdI EagI
EciI EcoICRI EcoRI FseI FspI FspAI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PmeI PmlI PpiI PspOMI PspXI PvuI
SacI SacII SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SrfI SwaI XhoI XmaI ZraI