Restriction map for
pFN21AE3825
(Linear) (Six-base) MAPSORT of: KIEE3825.seq Check: 5412 from: 1 to: 7830
KIEE3825.orf
With 182 enzymes: SgfI *
October 17, 2012 17:59 ..
AatII G_ACGT'C
Cuts at: 0 3792 7830
Size: 3792 4038
AccI GT'mk_AC
Cuts at: 0 2874 2931 3541 7830
Size: 2874 57 610 4289
Fragments arranged by size:
4289 2874 610 57
Acc65I G'GTAC_C
Cuts at: 0 5199 7830
Size: 5199 2631
AclI AA'CG_TT
Cuts at: 0 1876 7830
Size: 1876 5954
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1420 1688 4514 5986 6929 7160 7198 7425
Size: 1420 268 2826 1472 943 231 38 227
Cuts at: 7425 7830
Size: 405
Fragments arranged by size:
2826 1472 1420 943 405 268 231 227
38
AfeI AGC'GCT
Cuts at: 0 1081 7830
Size: 1081 6749
AflII C'TTAA_G
Cuts at: 0 7747 7830
Size: 7747 83
AflIII A'CryG_T
Cuts at: 0 139 2281 3633 6253 7540 7732 7830
Size: 139 2142 1352 2620 1287 192 98
Fragments arranged by size:
2620 2142 1352 1287 192 139 98
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5774 6470 7830
Size: 5774 696 1360
Fragments arranged by size:
5774 1360 696
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 455 487 7830
Size: 455 32 7343
Fragments arranged by size:
7343 455 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3810 4258 4483 5767 6009 7195 7830
Size: 3810 448 225 1284 242 1186 635
Fragments arranged by size:
3810 1284 1186 635 448 242 225
ApaI G_GGCC'C
Cuts at: 0 4578 7830
Size: 4578 3252
ApaLI G'TGCA_C
Cuts at: 0 3014 7830
Size: 3014 4816
ApoI r'AATT_y
Cuts at: 0 176 554 1167 1237 2167 2425 3183 3985
Size: 176 378 613 70 930 258 758 802
Cuts at: 3985 6103 6484 6550 6746 6976 7025 7115 7230
Size: 2118 381 66 196 230 49 90 115
Cuts at: 7230 7830
Size: 600
Fragments arranged by size:
2118 930 802 758 613 600 381 378
258 230 196 176 115 90 70 66
49
AvaI C'yCGr_G
Cuts at: 0 1400 2192 2510 7830
Size: 1400 792 318 5320
Fragments arranged by size:
5320 1400 792 318
BamHI G'GATC_C
Cuts at: 0 2959 7830
Size: 2959 4871
BanI G'GyrC_C
Cuts at: 0 1548 3197 5199 6268 7109 7830
Size: 1548 1649 2002 1069 841 721
Fragments arranged by size:
2002 1649 1548 1069 841 721
BanII G_rGCy'C
Cuts at: 0 618 789 1529 2193 3834 4578 4588 5024
Size: 618 171 740 664 1641 744 10 436
Cuts at: 5024 5414 5609 6177 7830
Size: 390 195 568 1653
Fragments arranged by size:
1653 1641 744 740 664 618 568 436
390 195 171 10
BbeI G_GCGC'C
Cuts at: 0 6272 7113 7830
Size: 6272 841 717
Fragments arranged by size:
6272 841 717
BbsI GAAGACnn'nnnn_
Cuts at: 0 1320 2674 3350 7046 7583 7830
Size: 1320 1354 676 3696 537 247
Fragments arranged by size:
3696 1354 1320 676 537 247
BciVI GTATCCnnnnn_n'
Cuts at: 0 3519 7830
Size: 3519 4311
BfrBI ATG'CAT
Cuts at: 0 95 6764 7362 7830
Size: 95 6669 598 468
Fragments arranged by size:
6669 598 468 95
BglII A'GATC_T
Cuts at: 0 1359 3417 7830
Size: 1359 2058 4413
Fragments arranged by size:
4413 2058 1359
BlpI GC'TnA_GC
Cuts at: 0 5018 7830
Size: 5018 2812
Bme1580I G_kGCm'C
Cuts at: 0 3018 4578 7830
Size: 3018 1560 3252
Fragments arranged by size:
3252 3018 1560
BmgBI CAC'GTC
Cuts at: 0 6304 7830
Size: 6304 1526
BmrI ACTGGGnnnn_n'
Cuts at: 0 1212 7830
Size: 1212 6618
BmtI G_CTAG'C
Cuts at: 0 1846 7798 7830
Size: 1846 5952 32
Fragments arranged by size:
5952 1846 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 1427 2959 5228 6380 7830
Size: 182 1245 1532 2269 1152 1450
Fragments arranged by size:
2269 1532 1450 1245 1152 182
Bpu10I CC'TnA_GC
Cuts at: 0 5045 7526 7830
Size: 5045 2481 304
Fragments arranged by size:
5045 2481 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 1304 3979 4119 4599 6258 6367 6496
Size: 521 783 2675 140 480 1659 109 129
Cuts at: 6496 7236 7267 7830
Size: 740 31 563
Fragments arranged by size:
2675 1659 783 740 563 521 480 140
129 109 31
BsaI GGTCTCn'nnnn_
Cuts at: 0 3430 7830
Size: 3430 4400
BsaAI yAC'GTr
Cuts at: 0 6409 7543 7830
Size: 6409 1134 287
Fragments arranged by size:
6409 1134 287
BsaBI GATnn'nnATC
Cuts at: 0 5081 7830
Size: 5081 2749
BsaHI Gr'CG_yC
Cuts at: 0 3789 5124 5268 6269 7110 7830
Size: 3789 1335 144 1001 841 720
Fragments arranged by size:
3789 1335 1001 841 720 144
BsaWI w'CCGG_w
Cuts at: 0 870 1055 1539 2130 7830
Size: 870 185 484 591 5700
Fragments arranged by size:
5700 870 591 484 185
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 129 159 771 801 2552 2582 3821 3851
Size: 129 30 612 30 1751 30 1239 30
Cuts at: 3851 4247 4277 7830
Size: 396 30 3553
Fragments arranged by size:
3553 1751 1239 612 396 129 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 137 715 4122 4754 5119 7786 7830
Size: 137 578 3407 632 365 2667 44
Fragments arranged by size:
3407 2667 632 578 365 137 44
BseYI C'CCAG_C
Cuts at: 0 669 4570 7008 7530 7830
Size: 669 3901 2438 522 300
Fragments arranged by size:
3901 2438 669 522 300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1277 3467 6679 7830
Size: 1277 2190 3212 1151
Fragments arranged by size:
3212 2190 1277 1151
BsiHKAI G_wGCw'C
Cuts at: 0 198 618 789 2193 3018 3834 4588 4753
Size: 198 420 171 1404 825 816 754 165
Cuts at: 4753 4779 5024 5414 7830
Size: 26 245 390 2416
Fragments arranged by size:
2416 1404 825 816 754 420 390 245
198 171 165 26
BsmI GAATG_Cn'
Cuts at: 0 3405 5763 6764 7609 7830
Size: 3405 2358 1001 845 221
Fragments arranged by size:
3405 2358 1001 845 221
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3780 5115 6385 7830
Size: 3780 1335 1270 1445
Fragments arranged by size:
3780 1445 1335 1270
Bsp1286I G_dGCh'C
Cuts at: 0 198 618 789 1529 2193 3018 3834 4578
Size: 198 420 171 740 664 825 816 744
Cuts at: 4578 4588 4753 4779 5024 5414 5609 6177 7830
Size: 10 165 26 245 390 195 568 1653
Fragments arranged by size:
1653 825 816 744 740 664 568 420
390 245 198 195 171 165 26 10
BspEI T'CCGG_A
Cuts at: 0 1055 1539 2130 7830
Size: 1055 484 591 5700
Fragments arranged by size:
5700 1055 591 484
BspHI T'CATG_A
Cuts at: 0 6930 7778 7830
Size: 6930 848 52
Fragments arranged by size:
6930 848 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1873 5211 5756 7673 7830
Size: 1873 3338 545 1917 157
Fragments arranged by size:
3338 1917 1873 545 157
BsrBI CCG'CTC
Cuts at: 0 5156 7830
Size: 5156 2674
BsrDI GCAATG_nn'
Cuts at: 0 22 925 3393 4863 5577 5973 7830
Size: 22 903 2468 1470 714 396 1857
Fragments arranged by size:
2468 1857 1470 903 714 396 22
BsrGI T'GTAC_A
Cuts at: 0 5538 5624 6256 7830
Size: 5538 86 632 1574
Fragments arranged by size:
5538 1574 632 86
BssSI C'ACGA_G
Cuts at: 0 395 2555 3464 3833 5486 6869 7830
Size: 395 2160 909 369 1653 1383 961
Fragments arranged by size:
2160 1653 1383 961 909 395 369
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1605 1633 3256 5767 7830
Size: 1605 28 1623 2511 2063
Fragments arranged by size:
2511 2063 1623 1605 28
BstBI TT'CG_AA
Cuts at: 0 1110 1644 2895 3162 3701 7830
Size: 1110 534 1251 267 539 4129
Fragments arranged by size:
4129 1251 1110 539 534 267
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1451 1985 3420 6064 7830
Size: 1451 534 1435 2644 1766
Fragments arranged by size:
2644 1766 1451 1435 534
BstYI r'GATC_y
Cuts at: 0 1030 1359 1536 2140 2959 3417 7278 7830
Size: 1030 329 177 604 819 458 3861 552
Fragments arranged by size:
3861 1030 819 604 552 458 329 177
BstZ17I GTA'TAC
Cuts at: 0 3542 7830
Size: 3542 4288
Bsu36I CC'TnA_GG
Cuts at: 0 5951 7561 7830
Size: 5951 1610 269
Fragments arranged by size:
5951 1610 269
BtgI C'CryG_G
Cuts at: 0 4439 7830
Size: 4439 3391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 636 7830
Size: 636 7194
BtsI GCAGTG_nn'
Cuts at: 0 797 1736 2745 4716 6345 7830
Size: 797 939 1009 1971 1629 1485
Fragments arranged by size:
1971 1629 1485 1009 939 797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1709 1744 7272 7307 7830
Size: 1709 35 5528 35 523
Fragments arranged by size:
5528 1709 523 35 35
DraI TTT'AAA
Cuts at: 0 2535 2838 3374 6813 7079 7229 7830
Size: 2535 303 536 3439 266 150 601
Fragments arranged by size:
3439 2535 601 536 303 266 150
DraIII CAC_nnn'GTG
Cuts at: 0 3022 6874 7830
Size: 3022 3852 956
Fragments arranged by size:
3852 3022 956
EaeI y'GGCC_r
Cuts at: 0 1773 3333 4172 6755 7830
Size: 1773 1560 839 2583 1075
Fragments arranged by size:
2583 1773 1560 1075 839
EarI CTCTTCn'nnn_
Cuts at: 0 1904 4533 5240 5915 6953 7830
Size: 1904 2629 707 675 1038 877
Fragments arranged by size:
2629 1904 1038 877 707 675
EcoICRI GAG'CTC
Cuts at: 0 616 787 2191 3832 4586 5022 5412 7830
Size: 616 171 1404 1641 754 436 390 2418
Fragments arranged by size:
2418 1641 1404 754 616 436 390 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 1420 1427 1688 2959 4514 5228 5986
Size: 182 1238 7 261 1271 1555 714 758
Cuts at: 5986 6380 6929 7160 7198 7425 7830
Size: 394 549 231 38 227 405
Fragments arranged by size:
1555 1271 1238 758 714 549 405 394
261 231 227 182 38 7
EcoO109I rG'GnC_Cy
Cuts at: 0 1885 2245 4393 4574 4575 5396 6514 7830
Size: 1885 360 2148 181 1 821 1118 1316
Fragments arranged by size:
2148 1885 1316 1118 821 360 181 1
EcoRI G'AATT_C
Cuts at: 0 2167 6103 6550 7830
Size: 2167 3936 447 1280
Fragments arranged by size:
3936 2167 1280 447
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1648 2776 3790 4925 5751 6016 6056 7830
Size: 1648 1128 1014 1135 826 265 40 1774
Fragments arranged by size:
1774 1648 1135 1128 1014 826 265 40
HaeII r_GCGC'y
Cuts at: 0 1083 6272 7113 7791 7830
Size: 1083 5189 841 678 39
Fragments arranged by size:
5189 1083 841 678 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 378 410 694 726 4099 4131 4247 4279
Size: 378 32 284 32 3373 32 116 32
Cuts at: 4279 5358 5390 5438 5470 5510 5542 6451 6483
Size: 1079 32 48 32 40 32 909 32
Cuts at: 6483 6592 6624 6925 6955 6957 6987 7830
Size: 109 32 301 30 2 30 843
Fragments arranged by size:
3373 1079 909 843 378 301 284 116
109 48 40 32 32 32 32 32
32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 0 666 830 972 1874 2224 2812 2875 3581
Size: 666 164 142 902 350 588 63 706
Cuts at: 3581 4832 7830
Size: 1251 2998
Fragments arranged by size:
2998 1251 902 706 666 588 350 164
142 63
HindIII A'AGCT_T
Cuts at: 0 1007 3997 6911 6951 7830
Size: 1007 2990 2914 40 879
Fragments arranged by size:
2990 2914 1007 879 40
KasI G'GCGC_C
Cuts at: 0 6268 7109 7830
Size: 6268 841 721
Fragments arranged by size:
6268 841 721
KpnI G_GTAC'C
Cuts at: 0 5203 7830
Size: 5203 2627
MfeI C'AATT_G
Cuts at: 0 833 914 1142 4274 5554 7830
Size: 833 81 228 3132 1280 2276
Fragments arranged by size:
3132 2276 1280 833 228 81
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 2987 7830
Size: 878 2109 4843
Fragments arranged by size:
4843 2109 878
MscI TGG'CCA
Cuts at: 0 1775 4174 6757 7830
Size: 1775 2399 2583 1073
Fragments arranged by size:
2583 2399 1775 1073
MslI CAynn'nnrTG
Cuts at: 0 756 2280 2945 3203 3386 3464 3551 5620
Size: 756 1524 665 258 183 78 87 2069
Cuts at: 5620 7830
Size: 2210
Fragments arranged by size:
2210 2069 1524 756 665 258 183 87
78
MspA1I CmG'CkG
Cuts at: 0 3280 4357 7273 7830
Size: 3280 1077 2916 557
Fragments arranged by size:
3280 2916 1077 557
NarI GG'CG_CC
Cuts at: 0 6269 7110 7830
Size: 6269 841 720
Fragments arranged by size:
6269 841 720
NcoI C'CATG_G
Cuts at: 0 4439 7830
Size: 4439 3391
NdeI CA'TA_TG
Cuts at: 0 2724 7830
Size: 2724 5106
NheI G'CTAG_C
Cuts at: 0 1842 7794 7830
Size: 1842 5952 36
Fragments arranged by size:
5952 1842 36
NsiI A_TGCA'T
Cuts at: 0 97 6766 7364 7830
Size: 97 6669 598 466
Fragments arranged by size:
6669 598 466 97
NspI r_CATG'y
Cuts at: 0 69 99 143 761 1307 2285 2553 3637
Size: 69 30 44 618 546 978 268 1084
Cuts at: 3637 5695 6257 6457 7590 7736 7830
Size: 2058 562 200 1133 146 94
Fragments arranged by size:
2058 1133 1084 978 618 562 546 268
200 146 94 69 44 30
PasI CC'CwG_GG
Cuts at: 0 2415 5392 7830
Size: 2415 2977 2438
Fragments arranged by size:
2977 2438 2415
PciI A'CATG_T
Cuts at: 0 139 2281 3633 6253 7732 7830
Size: 139 2142 1352 2620 1479 98
Fragments arranged by size:
2620 2142 1479 1352 139 98
PflMI CCAn_nnn'nTGG
Cuts at: 0 1782 7830
Size: 1782 6048
PfoI T'CCnGG_A
Cuts at: 0 1748 3825 6645 7830
Size: 1748 2077 2820 1185
Fragments arranged by size:
2820 2077 1748 1185
PmlI CAC'GTG
Cuts at: 0 6409 7543 7830
Size: 6409 1134 287
Fragments arranged by size:
6409 1134 287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2493 2525 6386 6418 7830
Size: 2493 32 3861 32 1412
Fragments arranged by size:
3861 2493 1412 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1885 2245 5396 6514 7830
Size: 1885 360 3151 1118 1316
Fragments arranged by size:
3151 1885 1316 1118 360
PshAI GACnn'nnGTC
Cuts at: 0 3560 4952 7830
Size: 3560 1392 2878
Fragments arranged by size:
3560 2878 1392
PspOMI G'GGCC_C
Cuts at: 0 4574 7830
Size: 4574 3256
PspXI vC'TCGA_Gb
Cuts at: 0 2192 7830
Size: 2192 5638
PstI C_TGCA'G
Cuts at: 0 804 2406 3562 4128 5753 7830
Size: 804 1602 1156 566 1625 2077
Fragments arranged by size:
2077 1625 1602 1156 804 566
PvuII CAG'CTG
Cuts at: 0 3280 7273 7830
Size: 3280 3993 557
Fragments arranged by size:
3993 3280 557
SacI G_AGCT'C
Cuts at: 0 618 789 2193 3834 4588 5024 5414 7830
Size: 618 171 1404 1641 754 436 390 2416
Fragments arranged by size:
2416 1641 1404 754 618 436 390 171
SalI G'TCGA_C
Cuts at: 0 2873 7830
Size: 2873 4957
SanDI GG'GwC_CC
Cuts at: 0 5396 6514 7830
Size: 5396 1118 1316
Fragments arranged by size:
5396 1316 1118
SapI GCTCTTCn'nnn_
Cuts at: 0 4533 7830
Size: 4533 3297
ScaI AGT'ACT
Cuts at: 0 5007 7830
Size: 5007 2823
SexAI A'CCwGG_T
Cuts at: 0 765 2355 3018 4803 5343 7830
Size: 765 1590 663 1785 540 2487
Fragments arranged by size:
2487 1785 1590 765 663 540
SfcI C'TryA_G
Cuts at: 0 37 800 1034 2261 2402 3077 3558 4124
Size: 37 763 234 1227 141 675 481 566
Cuts at: 4124 4865 5684 5749 6917 7830
Size: 741 819 65 1168 913
Fragments arranged by size:
1227 1168 913 819 763 741 675 566
481 234 141 65 37
SfoI GGC'GCC
Cuts at: 0 6270 7111 7830
Size: 6270 841 719
Fragments arranged by size:
6270 841 719
SmlI C'TyrA_G
Cuts at: 0 536 1319 1400 2192 2510 3994 4134 4614
Size: 536 783 81 792 318 1484 140 480
Cuts at: 4614 6273 6382 6475 7251 7282 7747 7830
Size: 1659 109 93 776 31 465 83
Fragments arranged by size:
1659 1484 792 783 776 536 480 465
318 140 109 93 83 81 31
SpeI A'CTAG_T
Cuts at: 0 1725 7170 7830
Size: 1725 5445 660
Fragments arranged by size:
5445 1725 660
SphI G_CATG'C
Cuts at: 0 99 761 2553 7830
Size: 99 662 1792 5277
Fragments arranged by size:
5277 1792 662 99
SspI AAT'ATT
Cuts at: 0 2339 4969 6134 7762 7830
Size: 2339 2630 1165 1628 68
Fragments arranged by size:
2630 2339 1628 1165 68
StuI AGG'CCT
Cuts at: 0 3848 7830
Size: 3848 3982
StyI C'CwwG_G
Cuts at: 0 2048 4439 5706 6741 7830
Size: 2048 2391 1267 1035 1089
Fragments arranged by size:
2391 2048 1267 1089 1035
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 659 1566 3155 3420 4050 4762 6817 7830
Size: 659 907 1589 265 630 712 2055 1013
Fragments arranged by size:
2055 1589 1013 907 712 659 630 265
TatI w'GTAC_w
Cuts at: 0 391 2691 2910 5005 5431 5538 5624 6256
Size: 391 2300 219 2095 426 107 86 632
Cuts at: 6256 6892 7830
Size: 636 938
Fragments arranged by size:
2300 2095 938 636 632 426 391 219
107 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 771 803 6641 6673 7830
Size: 771 32 5838 32 1157
Fragments arranged by size:
5838 1157 771 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4827 5345 7830
Size: 4827 518 2485
Fragments arranged by size:
4827 2485 518
XbaI T'CTAG_A
Cuts at: 0 5376 7830
Size: 5376 2454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 5010 7830
Size: 5010 2820
XhoI C'TCGA_G
Cuts at: 0 1400 2192 2510 7830
Size: 1400 792 318 5320
Fragments arranged by size:
5320 1400 792 318
XmnI GAAnn'nnTTC
Cuts at: 0 5829 6980 7609 7830
Size: 5829 1151 629 221
Fragments arranged by size:
5829 1151 629 221
ZraI GAC'GTC
Cuts at: 0 3790 7830
Size: 3790 4040
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BciVI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NsiI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuII SacI SalI SanDI SapI ScaI
SexAI SfcI SfoI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AarI AgeI AhdI AleI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BclI BglI BplI BsiEI BsiWI
BsrFI BssHII BstEII ClaI DrdI EagI EciI EcoNI
EcoRV FseI FspI FspAI HpaI MluI NaeI NgoMIV
NotI NruI PacI PmeI PsiI PsrI PvuI RsrII
SacII SbfI SfiI SgfI SgrAI SmaI SnaBI SrfI
SwaI XmaI