(Linear) (Six-base) MAPSORT of: KIEE3807.seq  Check: 1892  from: 1  to: 999



KIEE3807.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:20  ..

AccI GT'mk_AC

Cuts at:      0     556     999 
   Size:        556     443
 
Acc65I G'GTAC_C

Cuts at:      0     127     999 
   Size:        127     872
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     595     999 
   Size:        595     404
 
AlwNI CAG_nnn'CTG

Cuts at:      0     469     999 
   Size:        469     530
 
ApoI r'AATT_y

Cuts at:      0     912     999 
   Size:        912      87
 
BanI G'GyrC_C

Cuts at:      0     127     999 
   Size:        127     872
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     300     918     999 
   Size:        300     618      81

  Fragments arranged by size: 

                 618     300      81
 
BbvCI CC'TCA_GC

Cuts at:      0     475     999 
   Size:        475     524
 
BclI T'GATC_A

Cuts at:      0       8      29     999 
   Size:          8      21     970

  Fragments arranged by size: 

                 970      21       8
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     391     573     999 
   Size:        391     182     426

  Fragments arranged by size: 

                 426     391     182
 
Bpu10I CC'TnA_GC

Cuts at:      0     475     999 
   Size:        475     524
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     717     999 
   Size:        717     282
 
BseYI C'CCAG_C

Cuts at:      0     407     428     633     999 
   Size:        407      21     205     366

  Fragments arranged by size: 

                 407     366     205      21
 
BspHI T'CATG_A

Cuts at:      0     510     999 
   Size:        510     489
 
BsrGI T'GTAC_A

Cuts at:      0     107     999 
   Size:        107     892
 
BstYI r'GATC_y

Cuts at:      0       3     999 
   Size:          3     996
 
BtsI GCAGTG_nn'

Cuts at:      0     601     999 
   Size:        601     398
 
DraI TTT'AAA

Cuts at:      0     714     999 
   Size:        714     285
 
DraIII CAC_nnn'GTG

Cuts at:      0     399     999 
   Size:        399     600
 
EaeI y'GGCC_r

Cuts at:      0     251     359     999 
   Size:        251     108     640

  Fragments arranged by size: 

                 640     251     108
 
EarI CTCTTCn'nnn_

Cuts at:      0     395     999 
   Size:        395     604
 
HincII GTy'rAC

Cuts at:      0     836     959     999 
   Size:        836     123      40

  Fragments arranged by size: 

                 836     123      40
 
KpnI G_GTAC'C

Cuts at:      0     131     999 
   Size:        131     868
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     751     999 
   Size:        751     248
 
MscI TGG'CCA

Cuts at:      0     253     999 
   Size:        253     746
 
MspA1I CmG'CkG

Cuts at:      0     469     999 
   Size:        469     530
 
SfcI C'TryA_G

Cuts at:      0     980     999 
   Size:        980      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     144     355     410     999 
   Size:        144     211      55     589

  Fragments arranged by size: 

                 589     211     144      55
 
TatI w'GTAC_w

Cuts at:      0     107     999 
   Size:        107     892
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     444     999 
   Size:        444     555
 

 Enzymes that do cut:

     AccI   Acc65I     AlfI    AlwNI     ApoI     BanI     BbsI    BbvCI
     BclI     BmrI   Bpu10I    BseRI    BseYI    BspHI    BsrGI    BstYI
     BtsI     DraI   DraIII     EaeI     EarI   HincII     KpnI     MmeI
     MscI   MspA1I     SfcI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     MfeI     MluI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI